LOCUS       sequence-348148-        4942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YOR092W. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YOR092W. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCfB8711
FEATURES             Location/Qualifiers
     source          1..4942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     3'UTR           1976..2101
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(2191..2209)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2192..2209)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2227..2243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2227..2243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2240..2262)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2251..2267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2275..2281,2282..2299,2300..2305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2320..2341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2458..2475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2629..3217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2709..2728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3388..4179,4180..4248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4011..4030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4249..4353)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4421..4439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4477..4499)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4599..4618
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4812..4834
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4826..4843
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4827..4843
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4850..4869
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4850..4868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           4900..4942
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 cagatcaatt cccggggctg aggcttaata tgacacacat cacactggga caagccatct
       61 gggcctctgt cagaccaatc atcaaaatct atctgattat tggtgtaggc ttcggtttat
      121 gcaagatgaa catcttaacc gttcaggcca caaggtccat ctctgatatc gttttaacaa
      181 ttctgcttcc ttgtttatca ttcaataaaa tcgtagcaaa tatagaagat aacgatatca
      241 aagatgtcgg cattatttgt ctgacgtctg ttatactgtt cgcaactggc ttggggtttg
      301 cctttattgt tcgcagtgtg ctgcctgttc ctaagagatg gcgtggtggt atacttgcag
      361 gaggtatgtt tcccaatatc agcgatctac ccatcgccta tttgcaatcc atggaccaag
      421 ggtttatatt tacagaagca gaaggtgaga aaggtgttgc caacgttatc atctttctgg
      481 ccatgttttt gatatgtgtt ttcaatttag gtggtttcag actaattgaa aacgatttcc
      541 attacaaagg tgatgacgat gaagaaaata cgttgaccaa tgatgattct gcccaacagc
      601 caacacaacc aattgaaggc aattcttctt cttcatccaa ccaagatatc ctgaaagagc
      661 ccaacgagtc taccgttcct aacagttcac aagcaagcta catttctgag aaaaataaaa
      721 aagaaaaaac tgaactctct gtccccaagc ctacacatac tgcccctcca gcaatagatg
      781 acaggagctc gaactcatcc gcggtggtct ctatcgatag cattacacac tcactacgca
      841 caaaccatgt ggatgcccaa tctgtaagcg agcttaacga tcctacttac agaactagaa
      901 gtcaaccaat agcatacact accgagtcaa gaacatcaca tgttcataat aaccgcagga
      961 attccatcac tggctcctta cgttccatcg atatgcgtga actacccgcg gaaggcatgt
     1021 cggatttaat tcgtgaatac tccaatgttg atcaatacgg tagaagaagg aaaagttcta
     1081 tcagctccca aggagcaccc tctgttttgc aggcagacgg tactatttct ccaaatttga
     1141 ctagaacctc cactttacaa agagtcaaaa cttccaacct aacaagaata atcacctcag
     1201 atgccactgt tagtaagaaa gatattgaga cttccggttc ctctttacca aaatggctac
     1261 agaaatttcc cttaacaaaa tttttcgtct tttttctaaa aaattgtcta aggccttgtt
     1321 ccatggctgt tatcctggct ttgattatcg cttttattcc ctgggtaaaa gcccttttcg
     1381 ttacgaccag taatacacca aaaatcaaac aagccccaga taatgcacct gctctaactt
     1441 tcattatgga ttttacatct tatgttggag ccgcttcagt tcccttcggt ttgattcttt
     1501 taggcgctac tcttggtaga ttgaaaattg gaaaattata ccctggtttc tggaaatccg
     1561 cagtggtatt agtctttctc agacaatgta tcatgccgat ctttggtgtc ttgtggtgtg
     1621 accgtctagt gaaagcggga tggctaaatt gggaaaacga caagatgtta ttgtttgtta
     1681 ccgccattac ttggaactta ccaacaatga ccaccttaat ctacttcact gcaagttata
     1741 cccctgagga cgaaactgaa cccgttcaaa tggaatgtac ctctttcttt ttgatgcttc
     1801 aatatccttt gatggtcgtt agtttaccat ttttggtgtc ttatttcatc aaagttcaaa
     1861 tgaaattatg aattaaacct cagcgaattc gtgagaaagc ttgggaatag gcctttcaga
     1921 tctgcccgag accatggatg aagtatctag aaatagcttg atctggttac cactaaacca
     1981 gcctcaagaa cacccgaatg gagtctctaa gctacataat accaacttac actttacaaa
     2041 atgttgtccc ccaaaatgta gccattcgta tctgctccta awaaaaaraa agtttcttca
     2101 cattctaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa mccccccccc ccccccctgc
     2161 aggcggccgc ctcgaggcta gcttgagtat tctatagtgt cacctaaata gcttggcgta
     2221 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2281 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2341 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2401 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4381 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4441 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4501 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4561 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4621 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     4681 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4741 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4801 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     4861 actatagggc gaattaattc gagctcggta cccagcttgc ttgttctttt tgcagaagct
     4921 cagaataaac gctcaacttt gg
//
