LOCUS       sequence-34815-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
  TITLE     Cerulean, Venus, and VenusY67C FRET reference standards.
  JOURNAL   Biophys J. 2006 Dec 15. 91(12):L99-L101.
  PUBMED    17040988
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys J. 
            2006 Dec 15. 91(12):L99-L101."
COMMENT     Sequence Label: C5A
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(177..428)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(219..240)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     568..587
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attaagctgc ataaacagtt aacaacaaca attgcattca ttttatgttt caggttcagg
       61 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      121 atgatcagtt atctagatcc ggtggatccc gggcccgcgg taccgtcgac tgcagccttg
      181 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      241 tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag gtagtggttg
      301 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      361 tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      421 tgcttgtcgg cggtgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      481 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      541 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      601 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      661 tggtcggggt agcgggcgaa gcactgcagg ccgcagccga gggtggtcac gagggtgggc
      721 cagggcacgg gcagcttgcc ggtggtgcag atgagcttca gggtcagctt gccgtaggtg
      781 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgttta
//
