LOCUS       sequence-348198-        9875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor with alternative targeting scope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Villiger L, Schmidheini L, Mathis N, Rothgangl T, Marquart K, 
            Schwank G
  TITLE     Replacing the SpCas9 HNH domain by deaminases generates compact base
            editors with an alternative targeting scope.
  JOURNAL   Mol Ther Nucleic Acids. 2021 Aug 26;26:502-510. doi: 
            10.1016/j.omtn.2021.08.025. eCollection 2021 Dec 3.
  PUBMED    34631280
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2021-08-26"; volume: "26:502-510. doi"; 
            pages: " 10.1016/j.omtn.2021.08.025. eCollection 2021 Dec 3"
COMMENT     Sequence Label: p2T-CMV-HNHx-ABEmax7.10HD-BlastR
FEATURES             Location/Qualifiers
     source          1..9875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             143..1858
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5021..5041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5050..5067
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5090..5107)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5096..5320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5391..5407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5391..5407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5404..5426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5415..5431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5439..5445,5446..5463,5464..5469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5484..5505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5622..5743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5633..5652)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5687..5706
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5901..6299)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6318..6365)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6413..6742)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6427..6562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6481..6500)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6727..6747
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6866..7053,7054..7065)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7126..7714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7206..7225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7885..8676,8677..8745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8508..8527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8746..8850)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8929..8948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9140..9151,9152..9289)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9298..9677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9678..9875,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9832..9852
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtcgggt cagacaagaa gtacagcatc ggcctggcca tcggcaccaa
      181 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
      241 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
      301 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
      361 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
      421 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
      481 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
      541 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
      601 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
      661 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
      721 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
      781 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
      841 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
      901 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
      961 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     1021 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     1081 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     1141 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     1201 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     1261 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     1321 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     1381 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     1441 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     1501 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     1561 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     1621 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     1681 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     1741 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     1801 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     1861 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     1921 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     1981 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     2041 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     2101 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     2161 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     2221 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     2281 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     2341 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     2401 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     2461 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcgg
     2521 aggatcctct gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc
     2581 aaagcgagca tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag
     2641 agtgatcgga gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga
     2701 gatcatggca ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac
     2761 cctgtatgtg acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat
     2821 cggaagagtg gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga
     2881 tgtgctgcac caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga
     2941 cgagtgcgcc gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca
     3001 gaagaaggca cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga
     3061 gacaccaggc acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc
     3121 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     3181 ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     3241 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     3301 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     3361 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     3421 cgtggtgttt ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct
     3481 gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     3541 tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa
     3601 ggcccagagc tccaccgact ccggaggaag acagctggtg gaaacccggc agatcacaaa
     3661 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     3721 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     3781 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     3841 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     3901 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     3961 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     4021 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     4081 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     4141 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     4201 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     4261 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     4321 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     4381 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     4441 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     4501 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     4561 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     4621 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     4681 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     4741 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     4801 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     4861 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     4921 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc
     4981 tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
     5041 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     5101 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5161 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5221 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5281 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5341 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5401 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5461 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5521 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5581 gcggggagag gcggtttgcg tattgggcgc tacagacatg ataagataca ttgatgagtt
     5641 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5701 tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact
     5761 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     5821 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct
     5881 gctccttggc cacgaagtgc ttagccctcc cacacataac cagagggcag caattcacga
     5941 atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat cccaggatgc
     6001 agatcgagaa gcacctgtcg gcaccgtccg caggggctca agatgcccct gttctcattt
     6061 ccgatcgcga cgatacaagt caggttgcca gctgccgcag cagcagcagt gcccagcacc
     6121 acgagttctg cacaaggtcc cccagtaaaa tgatatacat tgacaccagt gaagatgcgg
     6181 ccgtcgctag agagagctgc gctggcgacg ctgtagtctt cagagatggg gatgctgttg
     6241 attgtagccg ttgctctttc aatgagggtg gattcttctt gagacaaagg cttggccatg
     6301 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     6361 tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa
     6421 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg
     6481 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     6541 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     6601 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     6661 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6721 ttccacaccc taactgacac acattccaca gaattcactg actcgctgcg ctcggtcgtt
     6781 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6841 ggggataacg caggaaagaa catgtaagtg atctccaaaa aataagtact ttttgactgt
     6901 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     6961 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     7021 aagtacagta atcaagtaaa attactcaag tactttacac ctctgcatgt gagcaaaagg
     7081 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7561 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7741 gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     8041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8821 ttagaaaaat aaacaaatag gggttccacg cacatttccc cgaaaagtgc cacctgacgt
     8881 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     8941 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9001 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9061 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgggt
     9121 accggcatat ggttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa
     9181 gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt
     9241 acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta acgcgttgac
     9301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9841 gcggtaggcg tgtacggtgg gaggtctata taagc
//
