LOCUS       sequence-348210-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, N501Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-N501Y
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        514..717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     668..688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     762..781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        762..780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4659..4676)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4665..4889
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4935..5363
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5022..5041)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5232..5253
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5372..5392)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5377..5706
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5557..5692
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5619..5638
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5773..6567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5827..5846)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6437..6456
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6741..6862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6778..6797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6832..6851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6911..6927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6911..6927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6924..6946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6935..6951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6959..6965,6966..6983,6984..6989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7142..7159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7313..7901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7393..7412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8072..8863,8864..8932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8695..8714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8933..9037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9112..9131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
       61 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      121 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      721 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      781 gagacccaag ctggctagca tgtttgtctt cctggtcctg ctgcctctgg tctcgtctca
      841 gtgcgtgaac ctgactacta gaacccagct gcctcctgcc tatactaact ccttcacccg
      901 cggcgtgtac tacccagaca aggtgttccg cagctccgtg ctgcactcca cccaggatct
      961 gttcctgccc ttcttcagca acgtgacctg gttccacgcc atccacgtga gcggcaccaa
     1021 tggcaccaag cggttcgaca atcccgtgct gccattcaac gatggcgtgt acttcgcctc
     1081 caccgagaag agcaacatca tccgcggctg gatcttcggc accaccctgg actccaagac
     1141 ccagagcctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca
     1201 gttctgcaat gatccattcc tgggcgtgta ctaccacaag aacaataagt cctggatgga
     1261 gagcgagttc cgcgtgtaca gctccgccaa caattgcacc ttcgagtacg tgtcccagcc
     1321 cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgcgcg agttcgtgtt
     1381 caagaatatc gatggctact tcaagatcta ctccaagcac acccccatca acctggtgcg
     1441 cgacctgcca cagggcttca gcgccctgga gccactggtg gatctgccaa tcggcatcaa
     1501 catcaccagg ttccagaccc tgctggccct gcaccgcagc tacctgaccc caggcgacag
     1561 ctccagcgga tggaccgctg gagctgctgc ctactacgtg ggctacctgc agccccgcac
     1621 cttcctgctg aagtacaacg agaatggcac catcaccgac gccgtggatt gcgccctgga
     1681 tccactgtcc gagacaaagt gcaccctgaa gagcttcacc gtggagaagg gcatctacca
     1741 gacctccaat ttccgcgtgc agccaaccga gagcatcgtg cgcttcccca atatcaccaa
     1801 cctgtgccca ttcggcgagg tgttcaacgc taccaggttc gccagcgtgt acgcttggaa
     1861 tcgcaagcgc atctccaact gcgtggccga ctacagcgtg ctgtacaact ccgccagctt
     1921 ctccaccttc aagtgctacg gcgtgtcccc caccaagctg aatgatctgt gcttcaccaa
     1981 cgtgtacgcc gatagcttcg tgatcagggg cgacgaggtg cgccagatcg ctccaggaca
     2041 gaccggcaag atcgctgact acaattacaa gctgcccgac gatttcaccg gctgcgtgat
     2101 cgcctggaac tccaacaatc tggatagcaa agtgggcggc aactacaatt acctgtaccg
     2161 cctgttccgc aagtccaatc tgaagccatt cgagcgcgac atctccaccg agatctacca
     2221 ggctggaagc accccatgca atggagtgga gggcttcaac tgctacttcc ccctgcagag
     2281 ctacggcttc cagccaacct acggagtggg ataccagcca tacagggtgg tggtgctgtc
     2341 cttcgagctg ctgcacgctc cagctaccgt gtgcggacca aagaagagca ccaatctggt
     2401 gaagaacaag tgcgtgaact tcaatttcaa cggcctgacc ggaaccggcg tgctgaccga
     2461 gtccaacaag aagttcctgc cattccagca gttcggaagg gacatcgctg ataccaccga
     2521 cgccgtgcgc gacccacaga ccctggagat cctggatatc accccatgct ccttcggcgg
     2581 cgtgagcgtg atcaccccag gaaccaatac cagcaaccag gtggccgtgc tgtaccaggg
     2641 cgtgaattgc accgaggtgc cagtggctat ccacgctgat cagctgaccc caacctggcg
     2701 cgtgtacagc accggatcca acgtgttcca gacccgcgcc ggatgcctga tcggagctga
     2761 gcacgtgaac aattcctacg agtgcgacat cccaatcgga gctggaatct gcgccagcta
     2821 ccagacccag accaactccc caaggagggc tcgcagcgtg gccagccagt ccatcatcgc
     2881 ctacaccatg tccctgggcg ccgagaatag cgtggcctac agcaacaatt ccatcgccat
     2941 cccaaccaac ttcaccatct ccgtgaccac cgagatcctg cccgtgtcca tgaccaagac
     3001 cagcgtggac tgcaccatgt acatctgcgg cgattccacc gagtgcagca acctgctgct
     3061 gcagtacggc agcttctgca cccagctgaa tcgcgccctg accggaatcg ctgtggagca
     3121 ggataagaac acccaggagg tgttcgccca ggtgaagcag atctacaaga cccccccaat
     3181 caaggacttc ggcggcttca atttcagcca gatcctgccc gatccaagca agccctccaa
     3241 gcgcagcttc atcgaggacc tgctgttcaa caaggtgacc ctggccgatg ccggcttcat
     3301 caagcagtac ggcgattgcc tgggcgacat cgctgcccgc gacctgatct gcgcccagaa
     3361 gttcaatggc ctgaccgtgc tgccaccact gctgaccgat gagatgatcg ctcagtacac
     3421 ctccgccctg ctggccggaa ccatcaccag cggatggacc ttcggcgctg gagccgccct
     3481 gcagatcccc ttcgccatgc agatggccta ccgcttcaac ggcatcggcg tgacccagaa
     3541 tgtgctgtac gagaaccaga agctgatcgc caatcagttc aactccgcca tcggcaagat
     3601 ccaggactcc ctgtccagca ccgccagcgc cctgggcaag ctgcaggatg tggtgaatca
     3661 gaacgcccag gccctgaata ccctggtgaa gcagctgtcc agcaacttcg gcgccatctc
     3721 cagcgtgctg aatgatatcc tgagccgcct ggacaaggtg gaggctgagg tgcagatcga
     3781 taggctgatc accggccgcc tgcagtccct gcagacctac gtgacccagc agctgatcag
     3841 ggctgctgag atcagggcca gcgccaatct ggctgctacc aagatgtccg agtgcgtgct
     3901 gggacagagc aagagggtgg acttctgcgg caagggctac cacctgatgt ccttcccaca
     3961 gagcgcccca cacggagtgg tgttcctgca cgtgacctac gtgccagccc aggagaagaa
     4021 cttcaccacc gctccagcta tctgccacga tggcaaggct cacttcccac gcgagggcgt
     4081 gttcgtgtcc aacggcaccc actggttcgt gacccagcgc aatttctacg agccccagat
     4141 catcaccacc gacaatacct tcgtgagcgg caactgcgac gtggtcatcg gaatcgtgaa
     4201 caataccgtg tacgatcccc tgcagccaga gctggactcc ttcaaggagg agctggataa
     4261 gtacttcaag aatcacacca gccccgacgt ggatctgggc gacatctccg gcatcaatgc
     4321 cagcgtggtg aacatccaga aggagatcga ccgcctgaac gaggtggcca agaatctgaa
     4381 cgagtccctg atcgatctgc aggagctggg caagtacgag cagtacatca agtggccatg
     4441 gtacatctgg ctgggcttca tcgccggcct gatcgccatc gtgatggtga ccatcatgct
     4501 gtgctgcatg acctcctgct gcagctgcct gaagggctgc tgctcctgcg gcagctgctg
     4561 caagttcgat gaggacgata gcgagcccgt gctgaagggc gtcaaactgc actatacata
     4621 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4681 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4741 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4801 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4861 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4921 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4981 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5041 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5101 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5161 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5221 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5281 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5341 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     5401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5461 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5521 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5581 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5641 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5701 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5761 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5821 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5881 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5941 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6001 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6061 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6121 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6181 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6241 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6301 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6361 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6421 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6481 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6541 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6601 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6661 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6721 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6841 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9061 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     9121 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttg
//
