LOCUS       sequence-348212-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, D1118H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-D1118H
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        183..562
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        563..766
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     717..737
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     811..830
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        811..829
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4708..4725)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4714..4938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4984..5412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5071..5090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5281..5302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5421..5441)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5426..5755
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5606..5741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5668..5687
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5822..6616
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5876..5895)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6486..6505
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6790..6911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6827..6846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6881..6900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6960..6976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6960..6976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6973..6995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6984..7000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7008..7014,7015..7032,7033..7038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7053..7074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7191..7208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7362..7950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7442..7461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8121..8912,8913..8981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8744..8763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8982..9086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9161..9169,1..11))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
       61 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      121 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      181 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      241 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      301 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      361 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      421 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      481 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      541 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      601 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      661 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      721 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
      781 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
      841 tggctagcat gtttgtcttc ctggtcctgc tgcctctggt ctcgtctcag tgcgtgaacc
      901 tgactactag aacccagctg cctcctgcct atactaactc cttcacccgc ggcgtgtact
      961 acccagacaa ggtgttccgc agctccgtgc tgcactccac ccaggatctg ttcctgccct
     1021 tcttcagcaa cgtgacctgg ttccacgcca tccacgtgag cggcaccaat ggcaccaagc
     1081 ggttcgacaa tcccgtgctg ccattcaacg atggcgtgta cttcgcctcc accgagaaga
     1141 gcaacatcat ccgcggctgg atcttcggca ccaccctgga ctccaagacc cagagcctgc
     1201 tgatcgtgaa caatgccacc aacgtggtca tcaaggtgtg cgagttccag ttctgcaatg
     1261 atccattcct gggcgtgtac taccacaaga acaataagtc ctggatggag agcgagttcc
     1321 gcgtgtacag ctccgccaac aattgcacct tcgagtacgt gtcccagccc ttcctgatgg
     1381 acctggaggg caagcagggc aatttcaaga acctgcgcga gttcgtgttc aagaatatcg
     1441 atggctactt caagatctac tccaagcaca cccccatcaa cctggtgcgc gacctgccac
     1501 agggcttcag cgccctggag ccactggtgg atctgccaat cggcatcaac atcaccaggt
     1561 tccagaccct gctggccctg caccgcagct acctgacccc aggcgacagc tccagcggat
     1621 ggaccgctgg agctgctgcc tactacgtgg gctacctgca gccccgcacc ttcctgctga
     1681 agtacaacga gaatggcacc atcaccgacg ccgtggattg cgccctggat ccactgtccg
     1741 agacaaagtg caccctgaag agcttcaccg tggagaaggg catctaccag acctccaatt
     1801 tccgcgtgca gccaaccgag agcatcgtgc gcttccccaa tatcaccaac ctgtgcccat
     1861 tcggcgaggt gttcaacgct accaggttcg ccagcgtgta cgcttggaat cgcaagcgca
     1921 tctccaactg cgtggccgac tacagcgtgc tgtacaactc cgccagcttc tccaccttca
     1981 agtgctacgg cgtgtccccc accaagctga atgatctgtg cttcaccaac gtgtacgccg
     2041 atagcttcgt gatcaggggc gacgaggtgc gccagatcgc tccaggacag accggcaaga
     2101 tcgctgacta caattacaag ctgcccgacg atttcaccgg ctgcgtgatc gcctggaact
     2161 ccaacaatct ggatagcaaa gtgggcggca actacaatta cctgtaccgc ctgttccgca
     2221 agtccaatct gaagccattc gagcgcgaca tctccaccga gatctaccag gctggaagca
     2281 ccccatgcaa tggagtggag ggcttcaact gctacttccc cctgcagagc tacggcttcc
     2341 agccaaccaa cggagtggga taccagccat acagggtggt ggtgctgtcc ttcgagctgc
     2401 tgcacgctcc agctaccgtg tgcggaccaa agaagagcac caatctggtg aagaacaagt
     2461 gcgtgaactt caatttcaac ggcctgaccg gaaccggcgt gctgaccgag tccaacaaga
     2521 agttcctgcc attccagcag ttcggaaggg acatcgctga taccaccgac gccgtgcgcg
     2581 acccacagac cctggagatc ctggatatca ccccatgctc cttcggcggc gtgagcgtga
     2641 tcaccccagg aaccaatacc agcaaccagg tggccgtgct gtaccagggc gtgaattgca
     2701 ccgaggtgcc agtggctatc cacgctgatc agctgacccc aacctggcgc gtgtacagca
     2761 ccggatccaa cgtgttccag acccgcgccg gatgcctgat cggagctgag cacgtgaaca
     2821 attcctacga gtgcgacatc ccaatcggag ctggaatctg cgccagctac cagacccaga
     2881 ccaactcccc aaggagggct cgcagcgtgg ccagccagtc catcatcgcc tacaccatgt
     2941 ccctgggcgc cgagaatagc gtggcctaca gcaacaattc catcgccatc ccaaccaact
     3001 tcaccatctc cgtgaccacc gagatcctgc ccgtgtccat gaccaagacc agcgtggact
     3061 gcaccatgta catctgcggc gattccaccg agtgcagcaa cctgctgctg cagtacggca
     3121 gcttctgcac ccagctgaat cgcgccctga ccggaatcgc tgtggagcag gataagaaca
     3181 cccaggaggt gttcgcccag gtgaagcaga tctacaagac ccccccaatc aaggacttcg
     3241 gcggcttcaa tttcagccag atcctgcccg atccaagcaa gccctccaag cgcagcttca
     3301 tcgaggacct gctgttcaac aaggtgaccc tggccgatgc cggcttcatc aagcagtacg
     3361 gcgattgcct gggcgacatc gctgcccgcg acctgatctg cgcccagaag ttcaatggcc
     3421 tgaccgtgct gccaccactg ctgaccgatg agatgatcgc tcagtacacc tccgccctgc
     3481 tggccggaac catcaccagc ggatggacct tcggcgctgg agccgccctg cagatcccct
     3541 tcgccatgca gatggcctac cgcttcaacg gcatcggcgt gacccagaat gtgctgtacg
     3601 agaaccagaa gctgatcgcc aatcagttca actccgccat cggcaagatc caggactccc
     3661 tgtccagcac cgccagcgcc ctgggcaagc tgcaggatgt ggtgaatcag aacgcccagg
     3721 ccctgaatac cctggtgaag cagctgtcca gcaacttcgg cgccatctcc agcgtgctga
     3781 atgatatcct gagccgcctg gacaaggtgg aggctgaggt gcagatcgat aggctgatca
     3841 ccggccgcct gcagtccctg cagacctacg tgacccagca gctgatcagg gctgctgaga
     3901 tcagggccag cgccaatctg gctgctacca agatgtccga gtgcgtgctg ggacagagca
     3961 agagggtgga cttctgcggc aagggctacc acctgatgtc cttcccacag agcgccccac
     4021 acggagtggt gttcctgcac gtgacctacg tgccagccca ggagaagaac ttcaccaccg
     4081 ctccagctat ctgccacgat ggcaaggctc acttcccacg cgagggcgtg ttcgtgtcca
     4141 acggcaccca ctggttcgtg acccagcgca atttctacga gccccagatc atcaccaccc
     4201 acaatacctt cgtgagcggc aactgcgacg tggtcatcgg aatcgtgaac aataccgtgt
     4261 acgatcccct gcagccagag ctggactcct tcaaggagga gctggataag tacttcaaga
     4321 atcacaccag ccccgacgtg gatctgggcg acatctccgg catcaatgcc agcgtggtga
     4381 acatccagaa ggagatcgac cgcctgaacg aggtggccaa gaatctgaac gagtccctga
     4441 tcgatctgca ggagctgggc aagtacgagc agtacatcaa gtggccatgg tacatctggc
     4501 tgggcttcat cgccggcctg atcgccatcg tgatggtgac catcatgctg tgctgcatga
     4561 cctcctgctg cagctgcctg aagggctgct gctcctgcgg cagctgctgc aagttcgatg
     4621 aggacgatag cgagcccgtg ctgaagggcg tcaaactgca ctatacatag ctcgagtcta
     4681 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4741 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4801 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4861 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4921 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4981 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5161 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5281 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5341 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5401 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5581 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5761 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5821 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5881 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5941 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6001 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6061 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6121 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6181 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6241 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6301 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6361 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6421 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6481 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6541 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6601 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6661 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9121 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctg
//
