LOCUS       sequence-348214-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, P681H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-P681H
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        46..425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        426..629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     580..600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     674..693
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        674..692
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4571..4588)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4577..4801
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4847..5275
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4934..4953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5144..5165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5284..5304)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5289..5618
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5469..5604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5531..5550
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5685..6479
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5739..5758)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6349..6368
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6653..6774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6690..6709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6744..6763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6823..6839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6823..6839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6836..6858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6847..6863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6871..6877,6878..6895,6896..6901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6916..6937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7054..7071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7225..7813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7305..7324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7984..8775,8776..8844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8607..8626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8845..8949)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9024..9043)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
       61 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      421 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      481 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      541 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      601 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
      661 ggcttatcga aattaatacg actcactata gggagaccca agctggctag catgtttgtc
      721 ttcctggtcc tgctgcctct ggtctcgtct cagtgcgtga acctgactac tagaacccag
      781 ctgcctcctg cctatactaa ctccttcacc cgcggcgtgt actacccaga caaggtgttc
      841 cgcagctccg tgctgcactc cacccaggat ctgttcctgc ccttcttcag caacgtgacc
      901 tggttccacg ccatccacgt gagcggcacc aatggcacca agcggttcga caatcccgtg
      961 ctgccattca acgatggcgt gtacttcgcc tccaccgaga agagcaacat catccgcggc
     1021 tggatcttcg gcaccaccct ggactccaag acccagagcc tgctgatcgt gaacaatgcc
     1081 accaacgtgg tcatcaaggt gtgcgagttc cagttctgca atgatccatt cctgggcgtg
     1141 tactaccaca agaacaataa gtcctggatg gagagcgagt tccgcgtgta cagctccgcc
     1201 aacaattgca ccttcgagta cgtgtcccag cccttcctga tggacctgga gggcaagcag
     1261 ggcaatttca agaacctgcg cgagttcgtg ttcaagaata tcgatggcta cttcaagatc
     1321 tactccaagc acacccccat caacctggtg cgcgacctgc cacagggctt cagcgccctg
     1381 gagccactgg tggatctgcc aatcggcatc aacatcacca ggttccagac cctgctggcc
     1441 ctgcaccgca gctacctgac cccaggcgac agctccagcg gatggaccgc tggagctgct
     1501 gcctactacg tgggctacct gcagccccgc accttcctgc tgaagtacaa cgagaatggc
     1561 accatcaccg acgccgtgga ttgcgccctg gatccactgt ccgagacaaa gtgcaccctg
     1621 aagagcttca ccgtggagaa gggcatctac cagacctcca atttccgcgt gcagccaacc
     1681 gagagcatcg tgcgcttccc caatatcacc aacctgtgcc cattcggcga ggtgttcaac
     1741 gctaccaggt tcgccagcgt gtacgcttgg aatcgcaagc gcatctccaa ctgcgtggcc
     1801 gactacagcg tgctgtacaa ctccgccagc ttctccacct tcaagtgcta cggcgtgtcc
     1861 cccaccaagc tgaatgatct gtgcttcacc aacgtgtacg ccgatagctt cgtgatcagg
     1921 ggcgacgagg tgcgccagat cgctccagga cagaccggca agatcgctga ctacaattac
     1981 aagctgcccg acgatttcac cggctgcgtg atcgcctgga actccaacaa tctggatagc
     2041 aaagtgggcg gcaactacaa ttacctgtac cgcctgttcc gcaagtccaa tctgaagcca
     2101 ttcgagcgcg acatctccac cgagatctac caggctggaa gcaccccatg caatggagtg
     2161 gagggcttca actgctactt ccccctgcag agctacggct tccagccaac caacggagtg
     2221 ggataccagc catacagggt ggtggtgctg tccttcgagc tgctgcacgc tccagctacc
     2281 gtgtgcggac caaagaagag caccaatctg gtgaagaaca agtgcgtgaa cttcaatttc
     2341 aacggcctga ccggaaccgg cgtgctgacc gagtccaaca agaagttcct gccattccag
     2401 cagttcggaa gggacatcgc tgataccacc gacgccgtgc gcgacccaca gaccctggag
     2461 atcctggata tcaccccatg ctccttcggc ggcgtgagcg tgatcacccc aggaaccaat
     2521 accagcaacc aggtggccgt gctgtaccag ggcgtgaatt gcaccgaggt gccagtggct
     2581 atccacgctg atcagctgac cccaacctgg cgcgtgtaca gcaccggatc caacgtgttc
     2641 cagacccgcg ccggatgcct gatcggagct gagcacgtga acaattccta cgagtgcgac
     2701 atcccaatcg gagctggaat ctgcgccagc taccagaccc agaccaactc ccataggagg
     2761 gctcgcagcg tggccagcca gtccatcatc gcctacacca tgtccctggg cgccgagaat
     2821 agcgtggcct acagcaacaa ttccatcgcc atcccaacca acttcaccat ctccgtgacc
     2881 accgagatcc tgcccgtgtc catgaccaag accagcgtgg actgcaccat gtacatctgc
     2941 ggcgattcca ccgagtgcag caacctgctg ctgcagtacg gcagcttctg cacccagctg
     3001 aatcgcgccc tgaccggaat cgctgtggag caggataaga acacccagga ggtgttcgcc
     3061 caggtgaagc agatctacaa gaccccccca atcaaggact tcggcggctt caatttcagc
     3121 cagatcctgc ccgatccaag caagccctcc aagcgcagct tcatcgagga cctgctgttc
     3181 aacaaggtga ccctggccga tgccggcttc atcaagcagt acggcgattg cctgggcgac
     3241 atcgctgccc gcgacctgat ctgcgcccag aagttcaatg gcctgaccgt gctgccacca
     3301 ctgctgaccg atgagatgat cgctcagtac acctccgccc tgctggccgg aaccatcacc
     3361 agcggatgga ccttcggcgc tggagccgcc ctgcagatcc ccttcgccat gcagatggcc
     3421 taccgcttca acggcatcgg cgtgacccag aatgtgctgt acgagaacca gaagctgatc
     3481 gccaatcagt tcaactccgc catcggcaag atccaggact ccctgtccag caccgccagc
     3541 gccctgggca agctgcagga tgtggtgaat cagaacgccc aggccctgaa taccctggtg
     3601 aagcagctgt ccagcaactt cggcgccatc tccagcgtgc tgaatgatat cctgagccgc
     3661 ctggacaagg tggaggctga ggtgcagatc gataggctga tcaccggccg cctgcagtcc
     3721 ctgcagacct acgtgaccca gcagctgatc agggctgctg agatcagggc cagcgccaat
     3781 ctggctgcta ccaagatgtc cgagtgcgtg ctgggacaga gcaagagggt ggacttctgc
     3841 ggcaagggct accacctgat gtccttccca cagagcgccc cacacggagt ggtgttcctg
     3901 cacgtgacct acgtgccagc ccaggagaag aacttcacca ccgctccagc tatctgccac
     3961 gatggcaagg ctcacttccc acgcgagggc gtgttcgtgt ccaacggcac ccactggttc
     4021 gtgacccagc gcaatttcta cgagccccag atcatcacca ccgacaatac cttcgtgagc
     4081 ggcaactgcg acgtggtcat cggaatcgtg aacaataccg tgtacgatcc cctgcagcca
     4141 gagctggact ccttcaagga ggagctggat aagtacttca agaatcacac cagccccgac
     4201 gtggatctgg gcgacatctc cggcatcaat gccagcgtgg tgaacatcca gaaggagatc
     4261 gaccgcctga acgaggtggc caagaatctg aacgagtccc tgatcgatct gcaggagctg
     4321 ggcaagtacg agcagtacat caagtggcca tggtacatct ggctgggctt catcgccggc
     4381 ctgatcgcca tcgtgatggt gaccatcatg ctgtgctgca tgacctcctg ctgcagctgc
     4441 ctgaagggct gctgctcctg cggcagctgc tgcaagttcg atgaggacga tagcgagccc
     4501 gtgctgaagg gcgtcaaact gcactataca tagctcgagt ctagagggcc cgtttaaacc
     4561 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4621 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4681 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4741 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4801 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5281 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5341 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5521 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5581 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5641 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5701 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5761 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5821 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5881 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5941 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6001 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6061 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6121 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6181 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6241 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6301 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6361 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6421 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6481 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6541 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6601 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6781 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8941 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9001 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9061 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     9121 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggtta
//
