LOCUS       sequence-34822-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pLKO human shRNA 2 c-MAF
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    118..235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        284..794
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     713..731
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(816..835)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcttgtggaa ggacgaaaca ccggacctgg aagactacta ctggactcga gtccagtagt
       61 agtcttccag gttttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt
      121 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      181 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      241 ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc
      301 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
      361 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
      421 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
      481 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
      541 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
      601 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
      661 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
      721 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
      781 acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc
      841 gcctcgccac ccgcgacgac gtccccaagg gccgtaacgc aaccctcgcc gccgcg
//
