LOCUS       sequence-348229-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hyPE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Song M, Lim JM, Min S, Oh JS, Kim DY, Woo JS, Nishimasu H, Cho SR, 
            Yoon S, Kim HH
  TITLE     Generation of a more efficient prime editor 2 by addition of the 
            Rad51 DNA-binding domain.
  JOURNAL   Nat Commun. 2021 Sep 23;12(1):5617. doi: 10.1038/s41467-021-25928-2.
  PUBMED    34556671
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-23"; volume: "12(1):5617. doi"; pages: " 
            10.1038/s41467-021-25928-2"
COMMENT     Sequence Label: pCMV-hyPE2
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2493..2513)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2526..4109,4110..4112,4113..6626))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2526..6626)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6627..6647)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6696..6715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6697..6715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6757..6960)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6786..6806)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6961..7340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7512..7531
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7610..7714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7715..7783,7784..8575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7933..7952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8746..9334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9235..9254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9488..9505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9622..9643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9658..9663,9664..9681,9682..9688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9696..9712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9701..9723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9720..9736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9720..9736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9807..10031
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10020..10037
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(10060..10077)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(10086..10106)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgccagagg gtgatgaatt ttctatgagg agggtagagg tgtctggagt ctctgtgatg
       61 gctgcctttc gggccgcttg gtcagccatc cggttgcctc tagcctcggc gctgtgtccc
      121 ttttgatgtc ctggacaatg gattatgcta agtcttttgg gcagaaagag ggcttttagt
      181 agggccaaga tctcgtcttt atttttgatc tctttgcctt ctgatgtgag ccacccacgc
      241 cttctgtata tttctccatg gatatgggca gtagcaaaag cataacggct atcagtataa
      301 acatttagct tcttaccttc tgccatcttt agggcctggg tgagtgctat cagttcagcc
      361 cgctgagcgg atgtcccggc tggcagggct ttagcccaga ttacctcggt ctcggtggtc
      421 accgcagctc ccgccttacg ctgtccctct tgtaagagac tgcttccatc cgtgtaccag
      481 gtgtggtcgg cgtctgggag cggctggtcc gttaggtcgg gtcgggttcc gtgggcttcg
      541 gccaggatat caaggcagtt gtgttgcagc ccttcctcag gcagtgggag cagcgtagcc
      601 gggttcaggg ctaccaccgg tccgaactgg acccggtccg tgtccaaaag caaggcctga
      661 tagtgagtca tccgggcgtt ggaaagccag cggtcggggg gttgtttgac tagtgcctct
      721 actgcatggg gggccagaat gactagtggc tgtcccatgg ttagcttgcc tgcatccttt
      781 gtcagtacgg caatggctgc taccatccgt aggcaagggg gccacccagc tgctactggg
      841 tctagctttt tggacaggta ggccaccggc cgacgccaag gtcccagttt ttgcgttagg
      901 acacctttgg cgtagccctg cttctcgtcg acaaagagtt caaagggctt agtcaaatct
      961 ggcaacccca gggctggggc agttagaaga gcttgcttga tttcttgata ggccttttgt
     1021 tggtctgggc cccaattaaa cagagtcccc ggtttggtga gagggtacag gggggctgcc
     1081 atttctgcaa acccagggat gaagaggcga cagaagcctg ccttccctag gaactccctt
     1141 agttgtcgag gggtcttcgg agtaggctgc cccatcacag tctcttttct ggcctcagtc
     1201 agccatctct gaccctcttt tagaagatac cccagatact tgacctgttt ctggcaaatt
     1261 tgggctttct tggccgaggc ccgatacccg aggttcccta gggtttgtaa cagggcccga
     1321 gtaccttgtt ggcagtctag ctcagaagtg gcggccagca gtaagtcatc cacgtactgt
     1381 agcaggatca agtctgggtg ctggatccgg aagtctgcta ggtctctgtg cagtgcctca
     1441 ttaaacaggg tgggactgtt tttgaaaccc tgtgggagtc tggtccaggt caattgtcct
     1501 gagattccca tctctggatc tctccactca aaggcgaaga gaggctgact ggtggggtgg
     1561 agtctcaggc agaaaaaggc atcctttaaa tcaagcacag tgtaccactg gtgggacggt
     1621 gggagcccgc tcaagaggtt gtaagggttg ggcacggtgg ggtggatgtc ttccacccgc
     1681 ttgttgactt ctctcagatc ctggacaggc ctataatcat tagtccctgg tttcttaacg
     1741 ggtagcaggg gcgtgttcca gggggactgg cagggtacca gtattccctg gtccaacagt
     1801 ctctgtatgt ggggcttgat ccccagtctg gcttcttgtg acatggggta ttgttttatg
     1861 gacacggggg tagaggttgc tttcagaggt atgatcagag gagcttggcg aactgccagt
     1921 cccatgcccc cggtttccgc ccaggcctga ggaaaatcag acagccatgt ggaccctaga
     1981 gaaacatctg gctcttttga ggtctcatgt agccgatact catcttctat atttagggtg
     2041 ctgctgccgc cgctgctgcc gccactgctc tctggtgttg ctgactcgct tgttcctggt
     2101 gtctcgctgc cagaggatcc tccgctagat cctccagatt gaagtagttt gtcaagctct
     2161 ttggagccag tagtaatctg tatgatctct gaccgccttt ggtggaattc agttgcagtg
     2221 gtgaaaccca ttggaactaa tttagctgcc tcagccagaa ttttatcagc tttggcttca
     2281 ctaattccct taatatttat tagctccttc tttggcgcat aggcaacagc ctccacagta
     2341 tggaatccag cttcttccaa tttcttcaca tcgttggcat ttatgccaca ctgctctaac
     2401 cgtgaaatgg gttgtgggcc aaagctttct tcttccactg aagtatctgc atttgcttca
     2461 agctgcatct gcattgcaga tccggaacta ccgactttcc tcttcttctt gggagaacca
     2521 ccagagtcac ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc
     2581 tggtggatca gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg
     2641 gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg
     2701 atgatattct cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc
     2761 actttgtcca gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg
     2821 atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc
     2881 tcgggggagc ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg
     2941 gagggcaggg ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc
     3001 cggccgtttt ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc
     3061 acttctttgt agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt
     3121 tccatgatgg tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt
     3181 tccactttgg ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgccgtac
     3241 ttcttagggt cccagtcctt ctttctggcg atcagcttat cgctgttcct cttgggcagg
     3301 atagactctt tgctgaagcc gcctgtctgc acctcggtct ttttcacgat attcacttgg
     3361 ggcatgctca gcactttccg cacggtggca aaatcccggc ccttatccca cacgatctcc
     3421 ccggtttcgc cgtttgtctc gatcagaggc cgcttccgga tctcgccgtt ggccagggta
     3481 atctcggtct tgaaaaagtt catgatgttg ctgtagaaga agtacttggc ggtagccttg
     3541 ccgatttcct gctcgctctt ggcgatcatc ttccgcacgt cgtacacctt gtagtcgccg
     3601 tacacgaact cgctttccag cttagggtac tttttgatca gggcggttcc cacgacggcg
     3661 ttcaggtagg cgtcgtgggc gtggtggtag ttgttgatct cgcgcacttt gtaaaactgg
     3721 aaatccttcc ggaaatcgga caccagcttg gacttcaggg tgatcacttt cacttcccgg
     3781 atcagcttgt cattctcgtc gtacttagtg ttcatccggg agtccaggat ctgtgccacg
     3841 tgctttgtga tctgccgggt ttccaccagc tgtctcttga tgaagccggc cttatccagt
     3901 tcgctcaggc cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc
     3961 ttggcgttca gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc
     4021 acgttgtcgc tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag
     4081 tcgtccttca gaaagctctg aggcacgata gcgtccacat cgtagtcgga cagccggttg
     4141 atgtccagtt cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc
     4201 ttctcgttct gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc
     4261 tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg
     4321 gtggtctggt tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc
     4381 actttcacga gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg
     4441 ctgccggcca gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct
     4501 ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg
     4561 ttggcgaagc cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg
     4621 atgccgttga tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc
     4681 tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc
     4741 tctctgtcct caaacagtgt cagggtcagc acgatatctt ccagaatgtc ctcgttttcc
     4801 tcattgtcca ggaagtcctt gtccttgata attttcagca gatcgtggta tgtgcccagg
     4861 gaggcgttga accgatcttc cacgccggag atttccacgg agtcgaagca ctcgattttc
     4921 ttgaagtagt cctctttcag ctgcttcacg gtcactttcc ggttggtctt gaacagcagg
     4981 tccacgatgg cctttttctg ctcgccgctc aggaaggcgg gctttctcat tccctcggtc
     5041 acgtatttca ctttggtcag ctcgttatac acggtgaagt actcgtacag caggctgtgc
     5101 ttgggcagca ccttctcgtt gggcaggttc ttatcgaagt tggtcatccg ctcgatgaag
     5161 ctctgggcgg aagcgccctt gtccaccact tcctcgaagt tccagggggt gatggtttcc
     5221 tcgctctttc tggtcatcca ggcgaatctg ctgtttcccc tggccagagg gcccacgtag
     5281 taggggatgc ggaaggtcag gatcttctcg atcttttccc ggttgtcctt caggaatggg
     5341 taaaaatctt cctgccgccg cagaatggcg tgcagctctc ccaggtggat ctggtggggg
     5401 atgctgccgt tgtcgaaggt ccgctgcttc cgcagcaggt cctctctgtt cagcttcacg
     5461 agcagttcct cggtgccgtc catcttttcc aggatgggct tgatgaactt gtagaactct
     5521 tcctggctgg ctccgccgtc aatgtagccg gcgtagccgt tcttgctctg gtcgaagaaa
     5581 atctctttgt acttctcagg cagctgctgc cgcacgagag ctttcagcag ggtcaggtcc
     5641 tggtggtgct cgtcgtatct cttgatcata gaggcgctca ggggggcctt ggtgatctcg
     5701 gtgttcactc tcaggatgtc gctcagcagg atggcgtcgg acaggttctt ggcggccaga
     5761 aacaggtcgg cgtactggtc gccgatctgg gccagcaggt tgtccaggtc gtcgtcgtag
     5821 gtgtccttgc tcagctgcag tttggcatcc tcggccaggt cgaagttgct cttgaagttg
     5881 ggggtcaggc ccaggctcag ggcaatcagg tttccgaaca ggccattctt cttctcgccg
     5941 ggcagctggg cgatcagatt ttccagccgt ctgctcttgc tcagtctggc agacaggatg
     6001 gccttggcgt ccacgccgct ggcgttgatg gggttttcct cgaacagctg gttgtaggtc
     6061 tgcaccagct ggatgaacag cttgtccacg tcgctgttgt cggggttcag gtcgccctcg
     6121 atcaggaagt ggccccggaa cttgatcatg tgggccaggg ccagatagat cagccgcagg
     6181 tcggccttgt cggtgctgtc caccagtttc tttctcaggt ggtagatggt ggggtacttc
     6241 tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg ggtgccgctc gtgcttctta
     6301 tcctcttcca ccaggaagga ctcttccagt ctgtggaaga agctgtcgtc caccttggcc
     6361 atctcgttgc tgaagatctc ttgcagatag cagatccggt tcttccgtct ggtgtatctt
     6421 cttctggcgg ttctcttcag ccgggtggcc tcggctgttt cgccgctgtc gaacagcagg
     6481 gctccgatca ggttcttctt gatgctgtgc cggtcggtgt tgcccagcac cttgaatttc
     6541 ttgctgggca ccttgtactc gtcggtgatc acggcccagc ccacagagtt ggtgccgatg
     6601 tccaggccga tgctgtactt cttgtcgact ttccgcttct tctttggtga ctcgaactcg
     6661 cttccgtcgg ctgtccgttt catggtggcg gctctcccta tagtgagtcg tattagcggc
     6721 cgcggatctc tagcggatct gacggttcac taaaccagct ctgcttatat agacctccca
     6781 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     6841 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     6901 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     6961 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     7021 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     7081 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     7141 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     7201 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     7261 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     7321 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
     7381 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
     7441 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
     7501 gcttaactat gcggcatcag agcagattgt actgagagta gaccctaggg gatcgggaga
     7561 tcgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7621 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7681 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7741 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7801 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7861 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7921 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7981 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8041 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8101 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8161 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8221 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8281 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8341 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8401 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8461 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8521 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8581 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8701 gttccactga gtgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8881 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9541 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9601 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cctaggcttt
     9661 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9721 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtatcgagcc
     9781 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
     9841 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     9901 acacctactc agacaatgcg atgcaatttt ctcattttat taggaaagga cagtgggagt
     9961 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
    10021 agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa tggtgatggt gatgatgacc
    10081 ggttagactt tcctcttctt cttgggctcg aattcgctgc cgtcggcggt tctttttgag
//
