LOCUS       sequence-348232-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, S477N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-S477N
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(528..1319,1320..1388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1151..1170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1389..1493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1568..1587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1759..2138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2139..2342
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2293..2313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2387..2406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2387..2405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6284..6301)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6290..6514
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6560..6988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6647..6666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6857..6878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6997..7017)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7002..7331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7182..7317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7244..7263
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7398..8192
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7452..7471)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8062..8081
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8366..8487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8403..8422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8457..8476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8536..8552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8536..8552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8560..8576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8584..8590,8591..8608,8609..8614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8629..8650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8767..8784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8938..9169,1..357))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9018..9037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
       61 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1501 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     1561 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     1621 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     1681 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     1741 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     1801 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1861 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1921 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1981 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2041 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2101 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2161 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2221 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2281 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2341 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     2401 atagggagac ccaagctggc tagcatgttt gtcttcctgg tcctgctgcc tctggtctcg
     2461 tctcagtgcg tgaacctgac tactagaacc cagctgcctc ctgcctatac taactccttc
     2521 acccgcggcg tgtactaccc agacaaggtg ttccgcagct ccgtgctgca ctccacccag
     2581 gatctgttcc tgcccttctt cagcaacgtg acctggttcc acgccatcca cgtgagcggc
     2641 accaatggca ccaagcggtt cgacaatccc gtgctgccat tcaacgatgg cgtgtacttc
     2701 gcctccaccg agaagagcaa catcatccgc ggctggatct tcggcaccac cctggactcc
     2761 aagacccaga gcctgctgat cgtgaacaat gccaccaacg tggtcatcaa ggtgtgcgag
     2821 ttccagttct gcaatgatcc attcctgggc gtgtactacc acaagaacaa taagtcctgg
     2881 atggagagcg agttccgcgt gtacagctcc gccaacaatt gcaccttcga gtacgtgtcc
     2941 cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct gcgcgagttc
     3001 gtgttcaaga atatcgatgg ctacttcaag atctactcca agcacacccc catcaacctg
     3061 gtgcgcgacc tgccacaggg cttcagcgcc ctggagccac tggtggatct gccaatcggc
     3121 atcaacatca ccaggttcca gaccctgctg gccctgcacc gcagctacct gaccccaggc
     3181 gacagctcca gcggatggac cgctggagct gctgcctact acgtgggcta cctgcagccc
     3241 cgcaccttcc tgctgaagta caacgagaat ggcaccatca ccgacgccgt ggattgcgcc
     3301 ctggatccac tgtccgagac aaagtgcacc ctgaagagct tcaccgtgga gaagggcatc
     3361 taccagacct ccaatttccg cgtgcagcca accgagagca tcgtgcgctt ccccaatatc
     3421 accaacctgt gcccattcgg cgaggtgttc aacgctacca ggttcgccag cgtgtacgct
     3481 tggaatcgca agcgcatctc caactgcgtg gccgactaca gcgtgctgta caactccgcc
     3541 agcttctcca ccttcaagtg ctacggcgtg tcccccacca agctgaatga tctgtgcttc
     3601 accaacgtgt acgccgatag cttcgtgatc aggggcgacg aggtgcgcca gatcgctcca
     3661 ggacagaccg gcaagatcgc tgactacaat tacaagctgc ccgacgattt caccggctgc
     3721 gtgatcgcct ggaactccaa caatctggat agcaaagtgg gcggcaacta caattacctg
     3781 taccgcctgt tccgcaagtc caatctgaag ccattcgagc gcgacatctc caccgagatc
     3841 taccaggctg gaaatacccc atgcaatgga gtggagggct tcaactgcta cttccccctg
     3901 cagagctacg gcttccagcc aaccaacgga gtgggatacc agccatacag ggtggtggtg
     3961 ctgtccttcg agctgctgca cgctccagct accgtgtgcg gaccaaagaa gagcaccaat
     4021 ctggtgaaga acaagtgcgt gaacttcaat ttcaacggcc tgaccggaac cggcgtgctg
     4081 accgagtcca acaagaagtt cctgccattc cagcagttcg gaagggacat cgctgatacc
     4141 accgacgccg tgcgcgaccc acagaccctg gagatcctgg atatcacccc atgctccttc
     4201 ggcggcgtga gcgtgatcac cccaggaacc aataccagca accaggtggc cgtgctgtac
     4261 cagggcgtga attgcaccga ggtgccagtg gctatccacg ctgatcagct gaccccaacc
     4321 tggcgcgtgt acagcaccgg atccaacgtg ttccagaccc gcgccggatg cctgatcgga
     4381 gctgagcacg tgaacaattc ctacgagtgc gacatcccaa tcggagctgg aatctgcgcc
     4441 agctaccaga cccagaccaa ctccccaagg agggctcgca gcgtggccag ccagtccatc
     4501 atcgcctaca ccatgtccct gggcgccgag aatagcgtgg cctacagcaa caattccatc
     4561 gccatcccaa ccaacttcac catctccgtg accaccgaga tcctgcccgt gtccatgacc
     4621 aagaccagcg tggactgcac catgtacatc tgcggcgatt ccaccgagtg cagcaacctg
     4681 ctgctgcagt acggcagctt ctgcacccag ctgaatcgcg ccctgaccgg aatcgctgtg
     4741 gagcaggata agaacaccca ggaggtgttc gcccaggtga agcagatcta caagaccccc
     4801 ccaatcaagg acttcggcgg cttcaatttc agccagatcc tgcccgatcc aagcaagccc
     4861 tccaagcgca gcttcatcga ggacctgctg ttcaacaagg tgaccctggc cgatgccggc
     4921 ttcatcaagc agtacggcga ttgcctgggc gacatcgctg cccgcgacct gatctgcgcc
     4981 cagaagttca atggcctgac cgtgctgcca ccactgctga ccgatgagat gatcgctcag
     5041 tacacctccg ccctgctggc cggaaccatc accagcggat ggaccttcgg cgctggagcc
     5101 gccctgcaga tccccttcgc catgcagatg gcctaccgct tcaacggcat cggcgtgacc
     5161 cagaatgtgc tgtacgagaa ccagaagctg atcgccaatc agttcaactc cgccatcggc
     5221 aagatccagg actccctgtc cagcaccgcc agcgccctgg gcaagctgca ggatgtggtg
     5281 aatcagaacg cccaggccct gaataccctg gtgaagcagc tgtccagcaa cttcggcgcc
     5341 atctccagcg tgctgaatga tatcctgagc cgcctggaca aggtggaggc tgaggtgcag
     5401 atcgataggc tgatcaccgg ccgcctgcag tccctgcaga cctacgtgac ccagcagctg
     5461 atcagggctg ctgagatcag ggccagcgcc aatctggctg ctaccaagat gtccgagtgc
     5521 gtgctgggac agagcaagag ggtggacttc tgcggcaagg gctaccacct gatgtccttc
     5581 ccacagagcg ccccacacgg agtggtgttc ctgcacgtga cctacgtgcc agcccaggag
     5641 aagaacttca ccaccgctcc agctatctgc cacgatggca aggctcactt cccacgcgag
     5701 ggcgtgttcg tgtccaacgg cacccactgg ttcgtgaccc agcgcaattt ctacgagccc
     5761 cagatcatca ccaccgacaa taccttcgtg agcggcaact gcgacgtggt catcggaatc
     5821 gtgaacaata ccgtgtacga tcccctgcag ccagagctgg actccttcaa ggaggagctg
     5881 gataagtact tcaagaatca caccagcccc gacgtggatc tgggcgacat ctccggcatc
     5941 aatgccagcg tggtgaacat ccagaaggag atcgaccgcc tgaacgaggt ggccaagaat
     6001 ctgaacgagt ccctgatcga tctgcaggag ctgggcaagt acgagcagta catcaagtgg
     6061 ccatggtaca tctggctggg cttcatcgcc ggcctgatcg ccatcgtgat ggtgaccatc
     6121 atgctgtgct gcatgacctc ctgctgcagc tgcctgaagg gctgctgctc ctgcggcagc
     6181 tgctgcaagt tcgatgagga cgatagcgag cccgtgctga agggcgtcaa actgcactat
     6241 acatagctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     6301 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6361 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6421 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6481 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6541 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6901 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6961 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7081 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7141 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7261 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7321 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     7381 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7441 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7501 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7561 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7621 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7681 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7741 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7801 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7861 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7921 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7981 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8041 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8101 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8161 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8221 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8281 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8341 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8461 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtc
//
