LOCUS       sequence-348235-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, L452R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-L452R
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     782..801
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        782..800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4679..4696)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4685..4909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4955..5383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5042..5061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5252..5273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5392..5412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5397..5726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5577..5712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5639..5658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5793..6587
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5847..5866)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6457..6476
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6761..6882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6798..6817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6852..6871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6931..6947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6931..6947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6944..6966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6955..6971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6979..6985,6986..7003,7004..7009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7024..7045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7162..7179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7333..7921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7413..7432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8092..8883,8884..8952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8715..8734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8953..9057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9132..9151)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
       61 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      121 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa
      781 ttaatacgac tcactatagg gagacccaag ctggctagca tgtttgtctt cctggtcctg
      841 ctgcctctgg tctcgtctca gtgcgtgaac ctgactacta gaacccagct gcctcctgcc
      901 tatactaact ccttcacccg cggcgtgtac tacccagaca aggtgttccg cagctccgtg
      961 ctgcactcca cccaggatct gttcctgccc ttcttcagca acgtgacctg gttccacgcc
     1021 atccacgtga gcggcaccaa tggcaccaag cggttcgaca atcccgtgct gccattcaac
     1081 gatggcgtgt acttcgcctc caccgagaag agcaacatca tccgcggctg gatcttcggc
     1141 accaccctgg actccaagac ccagagcctg ctgatcgtga acaatgccac caacgtggtc
     1201 atcaaggtgt gcgagttcca gttctgcaat gatccattcc tgggcgtgta ctaccacaag
     1261 aacaataagt cctggatgga gagcgagttc cgcgtgtaca gctccgccaa caattgcacc
     1321 ttcgagtacg tgtcccagcc cttcctgatg gacctggagg gcaagcaggg caatttcaag
     1381 aacctgcgcg agttcgtgtt caagaatatc gatggctact tcaagatcta ctccaagcac
     1441 acccccatca acctggtgcg cgacctgcca cagggcttca gcgccctgga gccactggtg
     1501 gatctgccaa tcggcatcaa catcaccagg ttccagaccc tgctggccct gcaccgcagc
     1561 tacctgaccc caggcgacag ctccagcgga tggaccgctg gagctgctgc ctactacgtg
     1621 ggctacctgc agccccgcac cttcctgctg aagtacaacg agaatggcac catcaccgac
     1681 gccgtggatt gcgccctgga tccactgtcc gagacaaagt gcaccctgaa gagcttcacc
     1741 gtggagaagg gcatctacca gacctccaat ttccgcgtgc agccaaccga gagcatcgtg
     1801 cgcttcccca atatcaccaa cctgtgccca ttcggcgagg tgttcaacgc taccaggttc
     1861 gccagcgtgt acgcttggaa tcgcaagcgc atctccaact gcgtggccga ctacagcgtg
     1921 ctgtacaact ccgccagctt ctccaccttc aagtgctacg gcgtgtcccc caccaagctg
     1981 aatgatctgt gcttcaccaa cgtgtacgcc gatagcttcg tgatcagggg cgacgaggtg
     2041 cgccagatcg ctccaggaca gaccggcaag atcgctgact acaattacaa gctgcccgac
     2101 gatttcaccg gctgcgtgat cgcctggaac tccaacaatc tggatagcaa agtgggcggc
     2161 aactacaatt acaggtaccg cctgttccgc aagtccaatc tgaagccatt cgagcgcgac
     2221 atctccaccg agatctacca ggctggaagc accccatgca atggagtgga gggcttcaac
     2281 tgctacttcc ccctgcagag ctacggcttc cagccaacca acggagtggg ataccagcca
     2341 tacagggtgg tggtgctgtc cttcgagctg ctgcacgctc cagctaccgt gtgcggacca
     2401 aagaagagca ccaatctggt gaagaacaag tgcgtgaact tcaatttcaa cggcctgacc
     2461 ggaaccggcg tgctgaccga gtccaacaag aagttcctgc cattccagca gttcggaagg
     2521 gacatcgctg ataccaccga cgccgtgcgc gacccacaga ccctggagat cctggatatc
     2581 accccatgct ccttcggcgg cgtgagcgtg atcaccccag gaaccaatac cagcaaccag
     2641 gtggccgtgc tgtaccaggg cgtgaattgc accgaggtgc cagtggctat ccacgctgat
     2701 cagctgaccc caacctggcg cgtgtacagc accggatcca acgtgttcca gacccgcgcc
     2761 ggatgcctga tcggagctga gcacgtgaac aattcctacg agtgcgacat cccaatcgga
     2821 gctggaatct gcgccagcta ccagacccag accaactccc caaggagggc tcgcagcgtg
     2881 gccagccagt ccatcatcgc ctacaccatg tccctgggcg ccgagaatag cgtggcctac
     2941 agcaacaatt ccatcgccat cccaaccaac ttcaccatct ccgtgaccac cgagatcctg
     3001 cccgtgtcca tgaccaagac cagcgtggac tgcaccatgt acatctgcgg cgattccacc
     3061 gagtgcagca acctgctgct gcagtacggc agcttctgca cccagctgaa tcgcgccctg
     3121 accggaatcg ctgtggagca ggataagaac acccaggagg tgttcgccca ggtgaagcag
     3181 atctacaaga cccccccaat caaggacttc ggcggcttca atttcagcca gatcctgccc
     3241 gatccaagca agccctccaa gcgcagcttc atcgaggacc tgctgttcaa caaggtgacc
     3301 ctggccgatg ccggcttcat caagcagtac ggcgattgcc tgggcgacat cgctgcccgc
     3361 gacctgatct gcgcccagaa gttcaatggc ctgaccgtgc tgccaccact gctgaccgat
     3421 gagatgatcg ctcagtacac ctccgccctg ctggccggaa ccatcaccag cggatggacc
     3481 ttcggcgctg gagccgccct gcagatcccc ttcgccatgc agatggccta ccgcttcaac
     3541 ggcatcggcg tgacccagaa tgtgctgtac gagaaccaga agctgatcgc caatcagttc
     3601 aactccgcca tcggcaagat ccaggactcc ctgtccagca ccgccagcgc cctgggcaag
     3661 ctgcaggatg tggtgaatca gaacgcccag gccctgaata ccctggtgaa gcagctgtcc
     3721 agcaacttcg gcgccatctc cagcgtgctg aatgatatcc tgagccgcct ggacaaggtg
     3781 gaggctgagg tgcagatcga taggctgatc accggccgcc tgcagtccct gcagacctac
     3841 gtgacccagc agctgatcag ggctgctgag atcagggcca gcgccaatct ggctgctacc
     3901 aagatgtccg agtgcgtgct gggacagagc aagagggtgg acttctgcgg caagggctac
     3961 cacctgatgt ccttcccaca gagcgcccca cacggagtgg tgttcctgca cgtgacctac
     4021 gtgccagccc aggagaagaa cttcaccacc gctccagcta tctgccacga tggcaaggct
     4081 cacttcccac gcgagggcgt gttcgtgtcc aacggcaccc actggttcgt gacccagcgc
     4141 aatttctacg agccccagat catcaccacc gacaatacct tcgtgagcgg caactgcgac
     4201 gtggtcatcg gaatcgtgaa caataccgtg tacgatcccc tgcagccaga gctggactcc
     4261 ttcaaggagg agctggataa gtacttcaag aatcacacca gccccgacgt ggatctgggc
     4321 gacatctccg gcatcaatgc cagcgtggtg aacatccaga aggagatcga ccgcctgaac
     4381 gaggtggcca agaatctgaa cgagtccctg atcgatctgc aggagctggg caagtacgag
     4441 cagtacatca agtggccatg gtacatctgg ctgggcttca tcgccggcct gatcgccatc
     4501 gtgatggtga ccatcatgct gtgctgcatg acctcctgct gcagctgcct gaagggctgc
     4561 tgctcctgcg gcagctgctg caagttcgat gaggacgata gcgagcccgt gctgaagggc
     4621 gtcaaactgc actatacata gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     4681 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4741 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4801 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4861 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4921 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4981 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5041 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5101 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5161 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5221 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5281 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5341 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5401 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5461 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5521 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5641 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5701 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5761 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5821 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5881 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5941 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6001 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6061 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6121 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6181 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6241 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6301 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6361 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6421 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6481 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6541 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6601 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     6661 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6721 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6781 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6901 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6961 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7021 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7081 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7141 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7201 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7261 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7321 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7741 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8221 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8401 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8941 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9001 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9061 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     9121 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatc
//
