LOCUS       sequence-348236-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Cho H, Gonzales-Wartz KK, Huang D, Yuan M, Peterson M, Liang J, 
            Beutler N, Torres JL, Cong Y, Postnikova E, Bangaru S, Talana CA, 
            Shi W, Yang ES, Zhang Y, Leung K, Wang L, Peng L, Skinner J, Li S, 
            Wu NC, Liu H, Dacon C, Moyer T, Cohen M, Zhao M, Lee FE, Weinberg 
            RS, Douagi I, Gross R, Schmaljohn C, Pegu A, Mascola JR, Holbrook M,
            Nemazee D, Rogers TF, Ward AB, Wilson IA, Crompton PD, Tan J
  TITLE     Bispecific antibodies targeting distinct regions of the spike 
            protein potently neutralize SARS-CoV-2 variants of concern.
  JOURNAL   Sci Transl Med. 2021 Oct 20;13(616):eabj5413. doi: 
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20.
  PUBMED    34519517
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2021-10-20"; volume: "13(616):eabj5413. doi"; pages: "
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20"
COMMENT     Sequence Label: pcDNA3.3_SARS2_Lambda
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2002..2019)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(4522..4541)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4566..4614
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4816..5244
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4903..4922)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5113..5134
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5253..5273)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5258..5587
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5438..5573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5500..5519
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5654..6448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5708..5727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6318..6337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6624..6745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6661..6680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6715..6734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6794..6810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6794..6810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6807..6829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6818..6834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6842..6848,6849..6866,6867..6872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6887..6908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7025..7042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7196..7784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7276..7295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7955..8746,8747..8815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8578..8597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8816..8920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8995..9014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactgagctc cttaaggtta actctagaga attcggatcc atgtttgtgt
      721 tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc aggacccaac
      781 ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac aaggtgttca
      841 ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc aatgtgacct
      901 ggttccatgc catccatgtg tctggcacca atgtgatcaa gaggtttgac aaccctgtgc
      961 tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc atcaggggct
     1021 ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg aacaatgcca
     1081 ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc ctgggagtct
     1141 actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac tcctctgcca
     1201 acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag ggcaagcagg
     1261 gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac ttcaagattt
     1321 acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc tctgccttgg
     1381 aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc ctgctggctc
     1441 tgcacaattc ctcctctggc tggacagcag gagcagcagc ctactatgtg ggctacctcc
     1501 aaccaaggac cttcctgctg aaatacaatg agaatggcac catcacagat gctgtggact
     1561 gtgccctgga cccactgtct gagaccaagt gtaccctgaa atccttcaca gtggagaagg
     1621 gcatctacca gaccagcaac ttcagggtcc aaccaacaga gagcattgtg aggtttccaa
     1681 acatcaccaa cctgtgtcca tttggagagg tgttcaatgc caccaggttt gcctctgtct
     1741 atgcctggaa caggaagagg attagcaact gtgtggctga ctactctgtg ctctacaact
     1801 ctgcctcctt cagcaccttc aagtgttatg gagtgagccc aaccaaactg aatgacctgt
     1861 gtttcaccaa tgtctatgct gactcctttg tgattagggg agatgaggtg agacagattg
     1921 cccctggaca aacaggcaag attgctgact acaactacaa actgcctgat gacttcacag
     1981 gctgtgtgat tgcctggaac agcaacaacc tggacagcaa ggtgggaggc aactacaact
     2041 accagtacag actgttcagg aagagcaacc tgaaaccatt tgagagggac atcagcacag
     2101 agatttacca ggctggcagc acaccatgta atggagtgga gggcttcaac tgttactctc
     2161 cactccaatc ctatggcttc caaccaacca atggagtggg ctaccaacca tacagggtgg
     2221 tggtgctgtc ctttgaactg ctccatgccc ctgccacagt gtgtggacca aagaagagca
     2281 ccaacctggt gaagaacaag tgtgtgaact tcaacttcaa tggactgaca ggcacaggag
     2341 tgctgacaga gagcaacaag aagttcctgc cattccaaca gtttggcagg gacattgctg
     2401 acaccacaga tgctgtgagg gacccacaga ccttggagat tctggacatc acaccatgtt
     2461 cctttggagg agtgtctgtg attacacctg gcaccaacac cagcaaccag gtggctgtgc
     2521 tctaccaggg tgtgaactgt actgaggtgc ctgtggctat ccatgctgac caacttacac
     2581 caacctggag ggtctacagc acaggcagca atgtgttcca gaccagggct ggctgtctga
     2641 ttggagcaga gcatgtgaac aactcctatg agtgtgacat cccaattgga gcaggcatct
     2701 gtgcctccta ccagacccag accaacagcc caaggagggc aaggtctgtg gcaagccaga
     2761 gcatcattgc ctacacaatg agtctgggag cagagaactc tgtggcttac agcaacaaca
     2821 gcattgccat cccaaccaac ttcaccatct ctgtgaccac agaaattctg cctgtgagta
     2881 tgaccaagac ctctgtggac tgtacaatgt atatctgtgg agacagcaca gagtgtagca
     2941 acctgctgct ccaatatggc tccttctgta cccaacttaa cagggctctg acaggcattg
     3001 ctgtggaaca ggacaagaac acccaggagg tgtttgccca ggtgaagcag atttacaaga
     3061 cacctccaat caaggacttt ggaggcttca acttcagcca gattctgcct gacccaagca
     3121 agccaagcaa gaggtccttc attgaggacc tgctgttcaa caaggtgacc ctggctgatg
     3181 ctggcttcat caagcaatat ggagactgtc tgggagacat tgctgctagg gacctgattt
     3241 gtgcccagaa gttcaatgga ctgaacgtgc tgcctccact gctgacagat gagatgattg
     3301 cccaatacac ctctgccctg ctggctggca ccatcacctc tggctggacc tttggagcag
     3361 gagcagccct ccaaatccca tttgctatgc agatggctta caggttcaat ggcattggag
     3421 tgacccagaa tgtgctctat gagaaccaga aactgattgc caaccagttc aactctgcca
     3481 ttggcaagat tcaggactcc ctgtccagca cagcctctgc cctgggcaaa ctccaagatg
     3541 tggtgaacca gaatgcccag gctctgaaca ccctggtgaa gcaactttcc agcaactttg
     3601 gagccatctc ctctgtgctg aatgacatcc tgagcagact ggacaaggtg gaggctgagg
     3661 tccagattga cagactgatt acaggcagac tccaatccct ccaaacctat gtgacccaac
     3721 aacttatcag ggctgctgag attagggcat ctgccaacct ggctgccacc aagatgagtg
     3781 agtgtgtgct gggacaaagc aagagggtgg acttctgtgg caagggctac cacctgatga
     3841 gttttccaca gtctgcccct catggagtgg tgttcctgca tgtgacctat gtgcctgccc
     3901 aggagaagaa cttcaccaca gcccctgcca tctgccatga tggcaaggct cactttccaa
     3961 gggagggagt gtttgtgagc aatggcaccc actggtttgt gacccagagg aacttctatg
     4021 aaccacagat tatcaccaca gacaacacct ttgtgtctgg caactgtgat gtggtgattg
     4081 gcattgtgaa caacacagtc tatgacccac tccaacctga actggactcc ttcaaggagg
     4141 aactggacaa atacttcaag aaccacacca gccctgatgt ggacctggga gacatctctg
     4201 gcatcaatgc ctctgtggtg aacatccaga aggagattga cagactgaat gaggtggcta
     4261 agaacctgaa tgagtccctg attgacctcc aagaactggg caaatatgaa caatacatca
     4321 agtggccatg gtacatctgg ctgggcttca ttgctggact gattgccatt gtgatggtga
     4381 ccataatgct gtgttgtatg acctcctgtt gttcctgtct gaaaggctgt tgttcctgtg
     4441 gctcctgttg taagtgactc gagccatgga agcttgatat ctaactgact gaaccggtgg
     4501 taccaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     4561 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     4621 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4681 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4741 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct
     4801 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4861 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4921 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4981 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5041 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5101 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5161 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5221 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5521 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5581 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5641 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5701 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5761 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5821 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5881 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5941 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6001 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6061 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6121 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6181 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6241 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6301 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6361 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6421 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc
     6481 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6541 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6601 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6721 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8941 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9001 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9061 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9121 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     9181 gcgtt
//
