LOCUS       sequence-348240-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Cho H, Gonzales-Wartz KK, Huang D, Yuan M, Peterson M, Liang J, 
            Beutler N, Torres JL, Cong Y, Postnikova E, Bangaru S, Talana CA, 
            Shi W, Yang ES, Zhang Y, Leung K, Wang L, Peng L, Skinner J, Li S, 
            Wu NC, Liu H, Dacon C, Moyer T, Cohen M, Zhao M, Lee FE, Weinberg 
            RS, Douagi I, Gross R, Schmaljohn C, Pegu A, Mascola JR, Holbrook M,
            Nemazee D, Rogers TF, Ward AB, Wilson IA, Crompton PD, Tan J
  TITLE     Bispecific antibodies targeting distinct regions of the spike 
            protein potently neutralize SARS-CoV-2 variants of concern.
  JOURNAL   Sci Transl Med. 2021 Oct 20;13(616):eabj5413. doi: 
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20.
  PUBMED    34519517
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2021-10-20"; volume: "13(616):eabj5413. doi"; pages: "
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20"
COMMENT     Sequence Label: pcDNA3.3_SARS2_Mu
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     588..612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2030..2047)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(4550..4569)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4594..4642
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4844..5272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4931..4950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5141..5162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5281..5301)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5286..5615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5466..5601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5528..5547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5682..6476
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5736..5755)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6346..6365
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6652..6773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6689..6708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6743..6762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6822..6838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6822..6838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6835..6857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6846..6862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6870..6876,6877..6894,6895..6900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6915..6936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7053..7070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7224..7812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7304..7323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7983..8774,8775..8843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8606..8625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8844..8948)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9023..9042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      661 gaccgatcca gcctccggac tgagctcctt aaggttaact ctagagaatt cggatccatg
      721 tttgtgttcc tggtgctgct gccactggtg tccagccagt gtgtgaacct gaccaccagg
      781 acccaacttc ctcctgccta caccaactcc ttcaccaggg gagtctacta ccctgacaag
      841 gtgttcaggt cctctgtgct gcacagcacc caggacctgt tcctgccatt cttcagcaat
      901 gtgacctggt tccatgccat ccatgtgtct ggcaccaatg gcaccaagag gtttgacaac
      961 cctgtgctgc cattcaatga tggagtctac tttgccagca tagagaagag caacatcatc
     1021 aggggctgga tttttggcac caccctggac agcaagaccc agtccctgct gattgtgaac
     1081 aatgccacca atgtggtgat taaggtgtgt gagttccagt tctgtaatga cccattcctg
     1141 ggagtcagca accacaagaa caacaagtcc tggatggagt ctgagttcag ggtctactcc
     1201 tctgccaaca actgtacctt tgaatatgtg agccaaccat tcctgatgga cttggagggc
     1261 aagcagggca acttcaagaa cctgagggag tttgtgttca agaacattga tggctacttc
     1321 aagatttaca gcaaacacac accaatcaac ctggtgaggg acctgccaca gggcttctct
     1381 gccttggaac cactggtgga cctgccaatt ggcatcaaca tcaccaggtt ccagaccctg
     1441 ctggctctgc acaggtccta cctgacacct ggagactcct cctctggctg gacagcagga
     1501 gcagcagcct actatgtggg ctacctccaa ccaaggacct tcctgctgaa atacaatgag
     1561 aatggcacca tcacagatgc tgtggactgt gccctggacc cactgtctga gaccaagtgt
     1621 accctgaaat ccttcacagt ggagaagggc atctaccaga ccagcaactt cagggtccaa
     1681 ccaacagaga gcattgtgag gtttccaaac atcaccaacc tgtgtccatt tggagaggtg
     1741 ttcaatgcca ccaagtttgc ctctgtctat gcctggaaca ggaagaggat tagcaactgt
     1801 gtggctgact actctgtgct ctacaactct gcctccttca gcaccttcaa gtgttatgga
     1861 gtgagcccaa ccaaactgaa tgacctgtgt ttcaccaatg tctatgctga ctcctttgtg
     1921 attaggggag atgaggtgag acagattgcc cctggacaaa caggcaagat tgctgactac
     1981 aactacaaac tgcctgatga cttcacaggc tgtgtgattg cctggaacag caacaacctg
     2041 gacagcaagg tgggaggcaa ctacaactac ctctacagac tgttcaggaa gagcaacctg
     2101 aaaccatttg agagggacat cagcacagag atttaccagg ctggcagcac accatgtaat
     2161 ggagtgaagg gcttcaactg ttactttcca ctccaatcct atggcttcca accaacctat
     2221 ggagtgggct accaaccata cagggtggtg gtgctgtcct ttgaactgct ccatgcccct
     2281 gccacagtgt gtggaccaaa gaagagcacc aacctggtga agaacaagtg tgtgaacttc
     2341 aacttcaatg gactgacagg cacaggagtg ctgacagaga gcaacaagaa gttcctgcca
     2401 ttccaacagt ttggcaggga cattgctgac accacagatg ctgtgaggga cccacagacc
     2461 ttggagattc tggacatcac accatgttcc tttggaggag tgtctgtgat tacacctggc
     2521 accaacacca gcaaccaggt ggctgtgctc taccagggtg tgaactgtac tgaggtgcct
     2581 gtggctatcc atgctgacca acttacacca acctggaggg tctacagcac aggcagcaat
     2641 gtgttccaga ccagggctgg ctgtctgatt ggagcagagc atgtgaacaa ctcctatgag
     2701 tgtgacatcc caattggagc aggcatctgt gcctcctacc agacccagac caacagccat
     2761 aggagggcaa ggtctgtggc aagccagagc atcattgcct acacaatgag tctgggagca
     2821 gagaactctg tggcttacag caacaacagc attgccatcc caaccaactt caccatctct
     2881 gtgaccacag agattctgcc tgtgagtatg accaagacct ctgtggactg tacaatgtat
     2941 atctgtggag acagcacaga gtgtagcaac ctgctgctcc aatatggctc cttctgtacc
     3001 caacttaaca gggctctgac aggcattgct gtggaacagg acaagaacac ccaggaggtg
     3061 tttgcccagg tgaagcagat ttacaagaca cctccaatca aggactttgg aggcttcaac
     3121 ttcagccaga ttctgcctga cccaagcaag ccaagcaaga ggtccttcat tgaggacctg
     3181 ctgttcaaca aggtgaccct ggctgatgct ggcttcatca agcaatatgg agactgtctg
     3241 ggagacattg ctgccaggga cctgatttgt gcccagaagt tcaatggact gacagtgctg
     3301 cctccactgc tgacagatga gatgattgcc caatacacct ctgccctgct ggctggcacc
     3361 atcacctctg gctggacctt tggagcagga gcagccctcc aaatcccatt tgctatgcag
     3421 atggcttaca ggttcaatgg cattggagtg acccagaatg tgctctatga gaaccagaaa
     3481 ctgattgcca accagttcaa ctctgccatt ggcaagattc aggactccct gtccagcaca
     3541 gcctctgccc tgggcaaact ccaaaatgtg gtgaaccaga atgcccaggc tctgaacacc
     3601 ctggtgaagc aactttccag caactttgga gccatctcct ctgtgctgaa tgacatcctg
     3661 agcagactgg acaaggtgga ggctgaggtc cagattgaca gactgattac aggcagactc
     3721 caatccctcc aaacctatgt gacccaacaa cttatcaggg ctgctgagat tagggcatct
     3781 gccaacctgg ctgccaccaa gatgagtgag tgtgtgctgg gacaaagcaa gagggtggac
     3841 ttctgtggca agggctacca cctgatgagt tttccacagt ctgcccctca tggagtggtg
     3901 ttcctgcatg tgacctatgt gcctgcccag gagaagaact tcaccacagc ccctgccatc
     3961 tgccatgatg gcaaggctca ctttccaagg gagggagtgt ttgtgagcaa tggcacccac
     4021 tggtttgtga cccagaggaa cttctatgaa ccacagatta tcaccacaga caacaccttt
     4081 gtgtctggca actgtgatgt ggtgattggc attgtgaaca acacagtcta tgacccactc
     4141 caacctgaac tggactcctt caaggaggaa ctggacaaat acttcaagaa ccacaccagc
     4201 cctgatgtgg acctgggaga catctctggc atcaatgcct ctgtggtgaa catccagaag
     4261 gagattgaca gactgaatga ggtggctaag aacctgaatg agtccctgat tgacctccaa
     4321 gaactgggca aatatgaaca atacatcaag tggccatggt acatctggct gggcttcatt
     4381 gctggactga ttgccattgt gatggtgacc ataatgctgt gttgtatgac ctcctgttgt
     4441 tcctgtctga aaggctgttg ttcctgtggc tcctgttgta agtgactcga gccatggaag
     4501 cttgatatct aactgactga accggtggta ccaaacgggg gaggctaact gaaacacgga
     4561 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4621 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4681 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4741 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4801 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5281 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5341 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5461 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5521 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5581 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5641 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5701 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5761 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5821 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5881 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5941 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6001 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6061 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6121 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6181 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6241 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6301 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6361 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6421 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6481 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6541 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6601 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6661 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6721 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6781 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6841 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6901 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6961 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7021 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7081 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7141 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7621 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7681 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7741 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7801 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7861 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7921 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7981 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8041 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8101 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8161 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8221 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8281 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8341 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8401 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8461 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8521 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8581 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8641 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8701 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8761 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8821 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8881 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8941 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9001 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9061 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     9121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     9181 tgcttcgcga tgtacgggcc agatat
//
