LOCUS       sequence-348241-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, K417N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-K417N
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(527..1318,1319..1387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1150..1169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1388..1492)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1567..1586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1758..2137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2138..2341
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2292..2312
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2386..2405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2386..2404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6283..6300)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6289..6513
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6559..6987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6646..6665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6856..6877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6996..7016)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7001..7330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7181..7316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7243..7262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7397..8191
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7451..7470)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8061..8080
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8365..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8402..8421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8456..8475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8535..8551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8535..8551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8548..8570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8559..8575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8583..8589,8590..8607,8608..8613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8628..8649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8766..8783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8937..9169,1..356))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9017..9036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
       61 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      121 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      181 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      241 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      301 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      361 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      421 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      481 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      541 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      601 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      661 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      721 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      781 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      841 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      901 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      961 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1021 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1081 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1141 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1201 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1261 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1321 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1381 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1441 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1501 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     1561 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     1621 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     1681 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     1741 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     1801 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1861 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1921 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1981 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2041 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2101 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2161 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2221 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2281 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2341 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
     2401 tagggagacc caagctggct agcatgtttg tcttcctggt cctgctgcct ctggtctcgt
     2461 ctcagtgcgt gaacctgact actagaaccc agctgcctcc tgcctatact aactccttca
     2521 cccgcggcgt gtactaccca gacaaggtgt tccgcagctc cgtgctgcac tccacccagg
     2581 atctgttcct gcccttcttc agcaacgtga cctggttcca cgccatccac gtgagcggca
     2641 ccaatggcac caagcggttc gacaatcccg tgctgccatt caacgatggc gtgtacttcg
     2701 cctccaccga gaagagcaac atcatccgcg gctggatctt cggcaccacc ctggactcca
     2761 agacccagag cctgctgatc gtgaacaatg ccaccaacgt ggtcatcaag gtgtgcgagt
     2821 tccagttctg caatgatcca ttcctgggcg tgtactacca caagaacaat aagtcctgga
     2881 tggagagcga gttccgcgtg tacagctccg ccaacaattg caccttcgag tacgtgtccc
     2941 agcccttcct gatggacctg gagggcaagc agggcaattt caagaacctg cgcgagttcg
     3001 tgttcaagaa tatcgatggc tacttcaaga tctactccaa gcacaccccc atcaacctgg
     3061 tgcgcgacct gccacagggc ttcagcgccc tggagccact ggtggatctg ccaatcggca
     3121 tcaacatcac caggttccag accctgctgg ccctgcaccg cagctacctg accccaggcg
     3181 acagctccag cggatggacc gctggagctg ctgcctacta cgtgggctac ctgcagcccc
     3241 gcaccttcct gctgaagtac aacgagaatg gcaccatcac cgacgccgtg gattgcgccc
     3301 tggatccact gtccgagaca aagtgcaccc tgaagagctt caccgtggag aagggcatct
     3361 accagacctc caatttccgc gtgcagccaa ccgagagcat cgtgcgcttc cccaatatca
     3421 ccaacctgtg cccattcggc gaggtgttca acgctaccag gttcgccagc gtgtacgctt
     3481 ggaatcgcaa gcgcatctcc aactgcgtgg ccgactacag cgtgctgtac aactccgcca
     3541 gcttctccac cttcaagtgc tacggcgtgt cccccaccaa gctgaatgat ctgtgcttca
     3601 ccaacgtgta cgccgatagc ttcgtgatca ggggcgacga ggtgcgccag atcgctccag
     3661 gacagaccgg caacatcgct gactacaatt acaagctgcc cgacgatttc accggctgcg
     3721 tgatcgcctg gaactccaac aatctggata gcaaagtggg cggcaactac aattacctgt
     3781 accgcctgtt ccgcaagtcc aatctgaagc cattcgagcg cgacatctcc accgagatct
     3841 accaggctgg aagcacccca tgcaatggag tggagggctt caactgctac ttccccctgc
     3901 agagctacgg cttccagcca accaacggag tgggatacca gccatacagg gtggtggtgc
     3961 tgtccttcga gctgctgcac gctccagcta ccgtgtgcgg accaaagaag agcaccaatc
     4021 tggtgaagaa caagtgcgtg aacttcaatt tcaacggcct gaccggaacc ggcgtgctga
     4081 ccgagtccaa caagaagttc ctgccattcc agcagttcgg aagggacatc gctgatacca
     4141 ccgacgccgt gcgcgaccca cagaccctgg agatcctgga tatcacccca tgctccttcg
     4201 gcggcgtgag cgtgatcacc ccaggaacca ataccagcaa ccaggtggcc gtgctgtacc
     4261 agggcgtgaa ttgcaccgag gtgccagtgg ctatccacgc tgatcagctg accccaacct
     4321 ggcgcgtgta cagcaccgga tccaacgtgt tccagacccg cgccggatgc ctgatcggag
     4381 ctgagcacgt gaacaattcc tacgagtgcg acatcccaat cggagctgga atctgcgcca
     4441 gctaccagac ccagaccaac tccccaagga gggctcgcag cgtggccagc cagtccatca
     4501 tcgcctacac catgtccctg ggcgccgaga atagcgtggc ctacagcaac aattccatcg
     4561 ccatcccaac caacttcacc atctccgtga ccaccgagat cctgcccgtg tccatgacca
     4621 agaccagcgt ggactgcacc atgtacatct gcggcgattc caccgagtgc agcaacctgc
     4681 tgctgcagta cggcagcttc tgcacccagc tgaatcgcgc cctgaccgga atcgctgtgg
     4741 agcaggataa gaacacccag gaggtgttcg cccaggtgaa gcagatctac aagacccccc
     4801 caatcaagga cttcggcggc ttcaatttca gccagatcct gcccgatcca agcaagccct
     4861 ccaagcgcag cttcatcgag gacctgctgt tcaacaaggt gaccctggcc gatgccggct
     4921 tcatcaagca gtacggcgat tgcctgggcg acatcgctgc ccgcgacctg atctgcgccc
     4981 agaagttcaa tggcctgacc gtgctgccac cactgctgac cgatgagatg atcgctcagt
     5041 acacctccgc cctgctggcc ggaaccatca ccagcggatg gaccttcggc gctggagccg
     5101 ccctgcagat ccccttcgcc atgcagatgg cctaccgctt caacggcatc ggcgtgaccc
     5161 agaatgtgct gtacgagaac cagaagctga tcgccaatca gttcaactcc gccatcggca
     5221 agatccagga ctccctgtcc agcaccgcca gcgccctggg caagctgcag gatgtggtga
     5281 atcagaacgc ccaggccctg aataccctgg tgaagcagct gtccagcaac ttcggcgcca
     5341 tctccagcgt gctgaatgat atcctgagcc gcctggacaa ggtggaggct gaggtgcaga
     5401 tcgataggct gatcaccggc cgcctgcagt ccctgcagac ctacgtgacc cagcagctga
     5461 tcagggctgc tgagatcagg gccagcgcca atctggctgc taccaagatg tccgagtgcg
     5521 tgctgggaca gagcaagagg gtggacttct gcggcaaggg ctaccacctg atgtccttcc
     5581 cacagagcgc cccacacgga gtggtgttcc tgcacgtgac ctacgtgcca gcccaggaga
     5641 agaacttcac caccgctcca gctatctgcc acgatggcaa ggctcacttc ccacgcgagg
     5701 gcgtgttcgt gtccaacggc acccactggt tcgtgaccca gcgcaatttc tacgagcccc
     5761 agatcatcac caccgacaat accttcgtga gcggcaactg cgacgtggtc atcggaatcg
     5821 tgaacaatac cgtgtacgat cccctgcagc cagagctgga ctccttcaag gaggagctgg
     5881 ataagtactt caagaatcac accagccccg acgtggatct gggcgacatc tccggcatca
     5941 atgccagcgt ggtgaacatc cagaaggaga tcgaccgcct gaacgaggtg gccaagaatc
     6001 tgaacgagtc cctgatcgat ctgcaggagc tgggcaagta cgagcagtac atcaagtggc
     6061 catggtacat ctggctgggc ttcatcgccg gcctgatcgc catcgtgatg gtgaccatca
     6121 tgctgtgctg catgacctcc tgctgcagct gcctgaaggg ctgctgctcc tgcggcagct
     6181 gctgcaagtt cgatgaggac gatagcgagc ccgtgctgaa gggcgtcaaa ctgcactata
     6241 catagctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     6301 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6361 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6421 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6481 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6541 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6601 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6661 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6721 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6781 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6841 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6901 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6961 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     7021 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7081 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7201 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7261 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7321 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     7381 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7441 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7501 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7561 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7621 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7681 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7741 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7801 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7861 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7921 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7981 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8041 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8101 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8161 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8221 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8281 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8341 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8461 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8521 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8581 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8641 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8701 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8761 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8821 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8881 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8941 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9001 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9061 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9121 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcg
//
