LOCUS       sequence-348243-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, W152C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-W152C
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        571..774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     819..838
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        819..837
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4716..4733)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4722..4946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4992..5420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5079..5098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5289..5310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5429..5449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5434..5763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5614..5749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5676..5695
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5830..6624
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5884..5903)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6494..6513
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6798..6919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6835..6854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6889..6908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6968..6984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6968..6984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6981..7003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6992..7008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7016..7022,7023..7040,7041..7046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7061..7082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7199..7216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7370..7958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7450..7469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8129..8920,8921..8989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8752..8771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8990..9094)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9169,1..19))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
       61 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      121 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      181 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      781 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      841 acccaagctg gctagcatgt ttgtcttcct ggtcctgctg cctctggtct cgtctcagtg
      901 cgtgaacctg actactagaa cccagctgcc tcctgcctat actaactcct tcacccgcgg
      961 cgtgtactac ccagacaagg tgttccgcag ctccgtgctg cactccaccc aggatctgtt
     1021 cctgcccttc ttcagcaacg tgacctggtt ccacgccatc cacgtgagcg gcaccaatgg
     1081 caccaagcgg ttcgacaatc ccgtgctgcc attcaacgat ggcgtgtact tcgcctccac
     1141 cgagaagagc aacatcatcc gcggctggat cttcggcacc accctggact ccaagaccca
     1201 gagcctgctg atcgtgaaca atgccaccaa cgtggtcatc aaggtgtgcg agttccagtt
     1261 ctgcaatgat ccattcctgg gcgtgtacta ccacaagaac aataagtcct gcatggagag
     1321 cgagttccgc gtgtacagct ccgccaacaa ttgcaccttc gagtacgtgt cccagccctt
     1381 cctgatggac ctggagggca agcagggcaa tttcaagaac ctgcgcgagt tcgtgttcaa
     1441 gaatatcgat ggctacttca agatctactc caagcacacc cccatcaacc tggtgcgcga
     1501 cctgccacag ggcttcagcg ccctggagcc actggtggat ctgccaatcg gcatcaacat
     1561 caccaggttc cagaccctgc tggccctgca ccgcagctac ctgaccccag gcgacagctc
     1621 cagcggatgg accgctggag ctgctgccta ctacgtgggc tacctgcagc cccgcacctt
     1681 cctgctgaag tacaacgaga atggcaccat caccgacgcc gtggattgcg ccctggatcc
     1741 actgtccgag acaaagtgca ccctgaagag cttcaccgtg gagaagggca tctaccagac
     1801 ctccaatttc cgcgtgcagc caaccgagag catcgtgcgc ttccccaata tcaccaacct
     1861 gtgcccattc ggcgaggtgt tcaacgctac caggttcgcc agcgtgtacg cttggaatcg
     1921 caagcgcatc tccaactgcg tggccgacta cagcgtgctg tacaactccg ccagcttctc
     1981 caccttcaag tgctacggcg tgtcccccac caagctgaat gatctgtgct tcaccaacgt
     2041 gtacgccgat agcttcgtga tcaggggcga cgaggtgcgc cagatcgctc caggacagac
     2101 cggcaagatc gctgactaca attacaagct gcccgacgat ttcaccggct gcgtgatcgc
     2161 ctggaactcc aacaatctgg atagcaaagt gggcggcaac tacaattacc tgtaccgcct
     2221 gttccgcaag tccaatctga agccattcga gcgcgacatc tccaccgaga tctaccaggc
     2281 tggaagcacc ccatgcaatg gagtggaggg cttcaactgc tacttccccc tgcagagcta
     2341 cggcttccag ccaaccaacg gagtgggata ccagccatac agggtggtgg tgctgtcctt
     2401 cgagctgctg cacgctccag ctaccgtgtg cggaccaaag aagagcacca atctggtgaa
     2461 gaacaagtgc gtgaacttca atttcaacgg cctgaccgga accggcgtgc tgaccgagtc
     2521 caacaagaag ttcctgccat tccagcagtt cggaagggac atcgctgata ccaccgacgc
     2581 cgtgcgcgac ccacagaccc tggagatcct ggatatcacc ccatgctcct tcggcggcgt
     2641 gagcgtgatc accccaggaa ccaataccag caaccaggtg gccgtgctgt accagggcgt
     2701 gaattgcacc gaggtgccag tggctatcca cgctgatcag ctgaccccaa cctggcgcgt
     2761 gtacagcacc ggatccaacg tgttccagac ccgcgccgga tgcctgatcg gagctgagca
     2821 cgtgaacaat tcctacgagt gcgacatccc aatcggagct ggaatctgcg ccagctacca
     2881 gacccagacc aactccccaa ggagggctcg cagcgtggcc agccagtcca tcatcgccta
     2941 caccatgtcc ctgggcgccg agaatagcgt ggcctacagc aacaattcca tcgccatccc
     3001 aaccaacttc accatctccg tgaccaccga gatcctgccc gtgtccatga ccaagaccag
     3061 cgtggactgc accatgtaca tctgcggcga ttccaccgag tgcagcaacc tgctgctgca
     3121 gtacggcagc ttctgcaccc agctgaatcg cgccctgacc ggaatcgctg tggagcagga
     3181 taagaacacc caggaggtgt tcgcccaggt gaagcagatc tacaagaccc ccccaatcaa
     3241 ggacttcggc ggcttcaatt tcagccagat cctgcccgat ccaagcaagc cctccaagcg
     3301 cagcttcatc gaggacctgc tgttcaacaa ggtgaccctg gccgatgccg gcttcatcaa
     3361 gcagtacggc gattgcctgg gcgacatcgc tgcccgcgac ctgatctgcg cccagaagtt
     3421 caatggcctg accgtgctgc caccactgct gaccgatgag atgatcgctc agtacacctc
     3481 cgccctgctg gccggaacca tcaccagcgg atggaccttc ggcgctggag ccgccctgca
     3541 gatccccttc gccatgcaga tggcctaccg cttcaacggc atcggcgtga cccagaatgt
     3601 gctgtacgag aaccagaagc tgatcgccaa tcagttcaac tccgccatcg gcaagatcca
     3661 ggactccctg tccagcaccg ccagcgccct gggcaagctg caggatgtgg tgaatcagaa
     3721 cgcccaggcc ctgaataccc tggtgaagca gctgtccagc aacttcggcg ccatctccag
     3781 cgtgctgaat gatatcctga gccgcctgga caaggtggag gctgaggtgc agatcgatag
     3841 gctgatcacc ggccgcctgc agtccctgca gacctacgtg acccagcagc tgatcagggc
     3901 tgctgagatc agggccagcg ccaatctggc tgctaccaag atgtccgagt gcgtgctggg
     3961 acagagcaag agggtggact tctgcggcaa gggctaccac ctgatgtcct tcccacagag
     4021 cgccccacac ggagtggtgt tcctgcacgt gacctacgtg ccagcccagg agaagaactt
     4081 caccaccgct ccagctatct gccacgatgg caaggctcac ttcccacgcg agggcgtgtt
     4141 cgtgtccaac ggcacccact ggttcgtgac ccagcgcaat ttctacgagc cccagatcat
     4201 caccaccgac aataccttcg tgagcggcaa ctgcgacgtg gtcatcggaa tcgtgaacaa
     4261 taccgtgtac gatcccctgc agccagagct ggactccttc aaggaggagc tggataagta
     4321 cttcaagaat cacaccagcc ccgacgtgga tctgggcgac atctccggca tcaatgccag
     4381 cgtggtgaac atccagaagg agatcgaccg cctgaacgag gtggccaaga atctgaacga
     4441 gtccctgatc gatctgcagg agctgggcaa gtacgagcag tacatcaagt ggccatggta
     4501 catctggctg ggcttcatcg ccggcctgat cgccatcgtg atggtgacca tcatgctgtg
     4561 ctgcatgacc tcctgctgca gctgcctgaa gggctgctgc tcctgcggca gctgctgcaa
     4621 gttcgatgag gacgatagcg agcccgtgct gaagggcgtc aaactgcact atacatagct
     4681 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4981 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5401 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5461 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5521 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5581 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5701 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5761 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5821 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5881 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5941 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6001 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6061 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6121 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6181 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6241 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6301 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6361 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6421 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6481 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6541 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6601 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6661 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6721 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6781 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6901 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6961 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7021 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7081 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7141 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7801 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9121 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagt
//
