LOCUS       sequence-348244-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, P26S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-P26S
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        495..698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     649..669
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     743..762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        743..761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4640..4657)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4646..4870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4916..5344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5003..5022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5213..5234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5353..5373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5358..5687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5538..5673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5600..5619
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5754..6548
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5808..5827)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6418..6437
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6722..6843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6759..6778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6813..6832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6892..6908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6892..6908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6905..6927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6916..6932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6940..6946,6947..6964,6965..6970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6985..7006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7123..7140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7294..7882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7374..7393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8053..8844,8845..8913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8676..8695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8914..9018)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9093..9112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
       61 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      121 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      181 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      481 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      541 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      601 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      661 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      721 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      781 atgtttgtct tcctggtcct gctgcctctg gtctcgtctc agtgcgtgaa cctgactact
      841 agaacccagc tgccttctgc ctatactaac tccttcaccc gcggcgtgta ctacccagac
      901 aaggtgttcc gcagctccgt gctgcactcc acccaggatc tgttcctgcc cttcttcagc
      961 aacgtgacct ggttccacgc catccacgtg agcggcacca atggcaccaa gcggttcgac
     1021 aatcccgtgc tgccattcaa cgatggcgtg tacttcgcct ccaccgagaa gagcaacatc
     1081 atccgcggct ggatcttcgg caccaccctg gactccaaga cccagagcct gctgatcgtg
     1141 aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttctgcaa tgatccattc
     1201 ctgggcgtgt actaccacaa gaacaataag tcctggatgg agagcgagtt ccgcgtgtac
     1261 agctccgcca acaattgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggag
     1321 ggcaagcagg gcaatttcaa gaacctgcgc gagttcgtgt tcaagaatat cgatggctac
     1381 ttcaagatct actccaagca cacccccatc aacctggtgc gcgacctgcc acagggcttc
     1441 agcgccctgg agccactggt ggatctgcca atcggcatca acatcaccag gttccagacc
     1501 ctgctggccc tgcaccgcag ctacctgacc ccaggcgaca gctccagcgg atggaccgct
     1561 ggagctgctg cctactacgt gggctacctg cagccccgca ccttcctgct gaagtacaac
     1621 gagaatggca ccatcaccga cgccgtggat tgcgccctgg atccactgtc cgagacaaag
     1681 tgcaccctga agagcttcac cgtggagaag ggcatctacc agacctccaa tttccgcgtg
     1741 cagccaaccg agagcatcgt gcgcttcccc aatatcacca acctgtgccc attcggcgag
     1801 gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga atcgcaagcg catctccaac
     1861 tgcgtggccg actacagcgt gctgtacaac tccgccagct tctccacctt caagtgctac
     1921 ggcgtgtccc ccaccaagct gaatgatctg tgcttcacca acgtgtacgc cgatagcttc
     1981 gtgatcaggg gcgacgaggt gcgccagatc gctccaggac agaccggcaa gatcgctgac
     2041 tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat
     2101 ctggatagca aagtgggcgg caactacaat tacctgtacc gcctgttccg caagtccaat
     2161 ctgaagccat tcgagcgcga catctccacc gagatctacc aggctggaag caccccatgc
     2221 aatggagtgg agggcttcaa ctgctacttc cccctgcaga gctacggctt ccagccaacc
     2281 aacggagtgg gataccagcc atacagggtg gtggtgctgt ccttcgagct gctgcacgct
     2341 ccagctaccg tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac
     2401 ttcaatttca acggcctgac cggaaccggc gtgctgaccg agtccaacaa gaagttcctg
     2461 ccattccagc agttcggaag ggacatcgct gataccaccg acgccgtgcg cgacccacag
     2521 accctggaga tcctggatat caccccatgc tccttcggcg gcgtgagcgt gatcacccca
     2581 ggaaccaata ccagcaacca ggtggccgtg ctgtaccagg gcgtgaattg caccgaggtg
     2641 ccagtggcta tccacgctga tcagctgacc ccaacctggc gcgtgtacag caccggatcc
     2701 aacgtgttcc agacccgcgc cggatgcctg atcggagctg agcacgtgaa caattcctac
     2761 gagtgcgaca tcccaatcgg agctggaatc tgcgccagct accagaccca gaccaactcc
     2821 ccaaggaggg ctcgcagcgt ggccagccag tccatcatcg cctacaccat gtccctgggc
     2881 gccgagaata gcgtggccta cagcaacaat tccatcgcca tcccaaccaa cttcaccatc
     2941 tccgtgacca ccgagatcct gcccgtgtcc atgaccaaga ccagcgtgga ctgcaccatg
     3001 tacatctgcg gcgattccac cgagtgcagc aacctgctgc tgcagtacgg cagcttctgc
     3061 acccagctga atcgcgccct gaccggaatc gctgtggagc aggataagaa cacccaggag
     3121 gtgttcgccc aggtgaagca gatctacaag acccccccaa tcaaggactt cggcggcttc
     3181 aatttcagcc agatcctgcc cgatccaagc aagccctcca agcgcagctt catcgaggac
     3241 ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta cggcgattgc
     3301 ctgggcgaca tcgctgcccg cgacctgatc tgcgcccaga agttcaatgg cctgaccgtg
     3361 ctgccaccac tgctgaccga tgagatgatc gctcagtaca cctccgccct gctggccgga
     3421 accatcacca gcggatggac cttcggcgct ggagccgccc tgcagatccc cttcgccatg
     3481 cagatggcct accgcttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag
     3541 aagctgatcg ccaatcagtt caactccgcc atcggcaaga tccaggactc cctgtccagc
     3601 accgccagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat
     3661 accctggtga agcagctgtc cagcaacttc ggcgccatct ccagcgtgct gaatgatatc
     3721 ctgagccgcc tggacaaggt ggaggctgag gtgcagatcg ataggctgat caccggccgc
     3781 ctgcagtccc tgcagaccta cgtgacccag cagctgatca gggctgctga gatcagggcc
     3841 agcgccaatc tggctgctac caagatgtcc gagtgcgtgc tgggacagag caagagggtg
     3901 gacttctgcg gcaagggcta ccacctgatg tccttcccac agagcgcccc acacggagtg
     3961 gtgttcctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac cgctccagct
     4021 atctgccacg atggcaaggc tcacttccca cgcgagggcg tgttcgtgtc caacggcacc
     4081 cactggttcg tgacccagcg caatttctac gagccccaga tcatcaccac cgacaatacc
     4141 ttcgtgagcg gcaactgcga cgtggtcatc ggaatcgtga acaataccgt gtacgatccc
     4201 ctgcagccag agctggactc cttcaaggag gagctggata agtacttcaa gaatcacacc
     4261 agccccgacg tggatctggg cgacatctcc ggcatcaatg ccagcgtggt gaacatccag
     4321 aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg
     4381 caggagctgg gcaagtacga gcagtacatc aagtggccat ggtacatctg gctgggcttc
     4441 atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgcat gacctcctgc
     4501 tgcagctgcc tgaagggctg ctgctcctgc ggcagctgct gcaagttcga tgaggacgat
     4561 agcgagcccg tgctgaaggg cgtcaaactg cactatacat agctcgagtc tagagggccc
     4621 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4681 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4741 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4801 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4861 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4921 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4981 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5041 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5101 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5161 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5221 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5281 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5341 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5521 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5581 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     5641 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     5701 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5761 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5821 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5881 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5941 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6001 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6061 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6121 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6181 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6241 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6301 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6361 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6421 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6481 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6541 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     6601 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6661 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6721 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6841 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9061 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9121 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcg
//
