LOCUS       sequence-348246-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, T20N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-T20N
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     667..686
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        667..685
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4564..4581)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4570..4794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4840..5268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4927..4946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5137..5158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5277..5297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5282..5611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5462..5597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5524..5543
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5678..6472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5732..5751)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6342..6361
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6646..6767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6683..6702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6737..6756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6816..6832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6816..6832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6829..6851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6840..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6864..6870,6871..6888,6889..6894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6909..6930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7047..7064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7218..7806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7298..7317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7977..8768,8769..8837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8600..8619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8838..8942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9017..9036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      661 cgaaattaat acgactcact atagggagac ccaagctggc tagcatgttt gtcttcctgg
      721 tcctgctgcc tctggtctcg tctcagtgcg tgaacctgac taatagaacc cagctgcctc
      781 ctgcctatac taactccttc acccgcggcg tgtactaccc agacaaggtg ttccgcagct
      841 ccgtgctgca ctccacccag gatctgttcc tgcccttctt cagcaacgtg acctggttcc
      901 acgccatcca cgtgagcggc accaatggca ccaagcggtt cgacaatccc gtgctgccat
      961 tcaacgatgg cgtgtacttc gcctccaccg agaagagcaa catcatccgc ggctggatct
     1021 tcggcaccac cctggactcc aagacccaga gcctgctgat cgtgaacaat gccaccaacg
     1081 tggtcatcaa ggtgtgcgag ttccagttct gcaatgatcc attcctgggc gtgtactacc
     1141 acaagaacaa taagtcctgg atggagagcg agttccgcgt gtacagctcc gccaacaatt
     1201 gcaccttcga gtacgtgtcc cagcccttcc tgatggacct ggagggcaag cagggcaatt
     1261 tcaagaacct gcgcgagttc gtgttcaaga atatcgatgg ctacttcaag atctactcca
     1321 agcacacccc catcaacctg gtgcgcgacc tgccacaggg cttcagcgcc ctggagccac
     1381 tggtggatct gccaatcggc atcaacatca ccaggttcca gaccctgctg gccctgcacc
     1441 gcagctacct gaccccaggc gacagctcca gcggatggac cgctggagct gctgcctact
     1501 acgtgggcta cctgcagccc cgcaccttcc tgctgaagta caacgagaat ggcaccatca
     1561 ccgacgccgt ggattgcgcc ctggatccac tgtccgagac aaagtgcacc ctgaagagct
     1621 tcaccgtgga gaagggcatc taccagacct ccaatttccg cgtgcagcca accgagagca
     1681 tcgtgcgctt ccccaatatc accaacctgt gcccattcgg cgaggtgttc aacgctacca
     1741 ggttcgccag cgtgtacgct tggaatcgca agcgcatctc caactgcgtg gccgactaca
     1801 gcgtgctgta caactccgcc agcttctcca ccttcaagtg ctacggcgtg tcccccacca
     1861 agctgaatga tctgtgcttc accaacgtgt acgccgatag cttcgtgatc aggggcgacg
     1921 aggtgcgcca gatcgctcca ggacagaccg gcaagatcgc tgactacaat tacaagctgc
     1981 ccgacgattt caccggctgc gtgatcgcct ggaactccaa caatctggat agcaaagtgg
     2041 gcggcaacta caattacctg taccgcctgt tccgcaagtc caatctgaag ccattcgagc
     2101 gcgacatctc caccgagatc taccaggctg gaagcacccc atgcaatgga gtggagggct
     2161 tcaactgcta cttccccctg cagagctacg gcttccagcc aaccaacgga gtgggatacc
     2221 agccatacag ggtggtggtg ctgtccttcg agctgctgca cgctccagct accgtgtgcg
     2281 gaccaaagaa gagcaccaat ctggtgaaga acaagtgcgt gaacttcaat ttcaacggcc
     2341 tgaccggaac cggcgtgctg accgagtcca acaagaagtt cctgccattc cagcagttcg
     2401 gaagggacat cgctgatacc accgacgccg tgcgcgaccc acagaccctg gagatcctgg
     2461 atatcacccc atgctccttc ggcggcgtga gcgtgatcac cccaggaacc aataccagca
     2521 accaggtggc cgtgctgtac cagggcgtga attgcaccga ggtgccagtg gctatccacg
     2581 ctgatcagct gaccccaacc tggcgcgtgt acagcaccgg atccaacgtg ttccagaccc
     2641 gcgccggatg cctgatcgga gctgagcacg tgaacaattc ctacgagtgc gacatcccaa
     2701 tcggagctgg aatctgcgcc agctaccaga cccagaccaa ctccccaagg agggctcgca
     2761 gcgtggccag ccagtccatc atcgcctaca ccatgtccct gggcgccgag aatagcgtgg
     2821 cctacagcaa caattccatc gccatcccaa ccaacttcac catctccgtg accaccgaga
     2881 tcctgcccgt gtccatgacc aagaccagcg tggactgcac catgtacatc tgcggcgatt
     2941 ccaccgagtg cagcaacctg ctgctgcagt acggcagctt ctgcacccag ctgaatcgcg
     3001 ccctgaccgg aatcgctgtg gagcaggata agaacaccca ggaggtgttc gcccaggtga
     3061 agcagatcta caagaccccc ccaatcaagg acttcggcgg cttcaatttc agccagatcc
     3121 tgcccgatcc aagcaagccc tccaagcgca gcttcatcga ggacctgctg ttcaacaagg
     3181 tgaccctggc cgatgccggc ttcatcaagc agtacggcga ttgcctgggc gacatcgctg
     3241 cccgcgacct gatctgcgcc cagaagttca atggcctgac cgtgctgcca ccactgctga
     3301 ccgatgagat gatcgctcag tacacctccg ccctgctggc cggaaccatc accagcggat
     3361 ggaccttcgg cgctggagcc gccctgcaga tccccttcgc catgcagatg gcctaccgct
     3421 tcaacggcat cggcgtgacc cagaatgtgc tgtacgagaa ccagaagctg atcgccaatc
     3481 agttcaactc cgccatcggc aagatccagg actccctgtc cagcaccgcc agcgccctgg
     3541 gcaagctgca ggatgtggtg aatcagaacg cccaggccct gaataccctg gtgaagcagc
     3601 tgtccagcaa cttcggcgcc atctccagcg tgctgaatga tatcctgagc cgcctggaca
     3661 aggtggaggc tgaggtgcag atcgataggc tgatcaccgg ccgcctgcag tccctgcaga
     3721 cctacgtgac ccagcagctg atcagggctg ctgagatcag ggccagcgcc aatctggctg
     3781 ctaccaagat gtccgagtgc gtgctgggac agagcaagag ggtggacttc tgcggcaagg
     3841 gctaccacct gatgtccttc ccacagagcg ccccacacgg agtggtgttc ctgcacgtga
     3901 cctacgtgcc agcccaggag aagaacttca ccaccgctcc agctatctgc cacgatggca
     3961 aggctcactt cccacgcgag ggcgtgttcg tgtccaacgg cacccactgg ttcgtgaccc
     4021 agcgcaattt ctacgagccc cagatcatca ccaccgacaa taccttcgtg agcggcaact
     4081 gcgacgtggt catcggaatc gtgaacaata ccgtgtacga tcccctgcag ccagagctgg
     4141 actccttcaa ggaggagctg gataagtact tcaagaatca caccagcccc gacgtggatc
     4201 tgggcgacat ctccggcatc aatgccagcg tggtgaacat ccagaaggag atcgaccgcc
     4261 tgaacgaggt ggccaagaat ctgaacgagt ccctgatcga tctgcaggag ctgggcaagt
     4321 acgagcagta catcaagtgg ccatggtaca tctggctggg cttcatcgcc ggcctgatcg
     4381 ccatcgtgat ggtgaccatc atgctgtgct gcatgacctc ctgctgcagc tgcctgaagg
     4441 gctgctgctc ctgcggcagc tgctgcaagt tcgatgagga cgatagcgag cccgtgctga
     4501 agggcgtcaa actgcactat acatagctcg agtctagagg gcccgtttaa acccgctgat
     4561 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4621 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4681 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4741 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4801 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4861 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4921 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4981 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5041 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5101 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5161 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5221 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5281 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5341 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5401 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5461 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5521 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     5581 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     5641 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5701 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5761 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5821 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5881 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5941 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6001 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6061 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6121 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6181 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6241 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6301 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6361 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6421 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6481 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6601 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6781 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8941 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     9001 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9061 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgttt
//
