LOCUS       sequence-348249-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (D614G, S13I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-S13I
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        108..487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        488..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     642..662
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     736..755
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        736..754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4633..4650)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4639..4863
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4909..5337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4996..5015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5206..5227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5346..5366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5351..5680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5531..5666
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5593..5612
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5747..6541
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5801..5820)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6411..6430
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6715..6836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6752..6771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6806..6825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6885..6901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6885..6901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6898..6920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6909..6925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6933..6939,6940..6957,6958..6963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6978..6999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7116..7133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7287..7875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7367..7386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8046..8837,8838..8906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8669..8688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8907..9011)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9086..9105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
       61 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      661 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      721 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcatgtttg
      781 tcttcctggt cctgctgcct ctggtctcga tacagtgcgt gaacctgact actagaaccc
      841 agctgcctcc tgcctatact aactccttca cccgcggcgt gtactaccca gacaaggtgt
      901 tccgcagctc cgtgctgcac tccacccagg atctgttcct gcccttcttc agcaacgtga
      961 cctggttcca cgccatccac gtgagcggca ccaatggcac caagcggttc gacaatcccg
     1021 tgctgccatt caacgatggc gtgtacttcg cctccaccga gaagagcaac atcatccgcg
     1081 gctggatctt cggcaccacc ctggactcca agacccagag cctgctgatc gtgaacaatg
     1141 ccaccaacgt ggtcatcaag gtgtgcgagt tccagttctg caatgatcca ttcctgggcg
     1201 tgtactacca caagaacaat aagtcctgga tggagagcga gttccgcgtg tacagctccg
     1261 ccaacaattg caccttcgag tacgtgtccc agcccttcct gatggacctg gagggcaagc
     1321 agggcaattt caagaacctg cgcgagttcg tgttcaagaa tatcgatggc tacttcaaga
     1381 tctactccaa gcacaccccc atcaacctgg tgcgcgacct gccacagggc ttcagcgccc
     1441 tggagccact ggtggatctg ccaatcggca tcaacatcac caggttccag accctgctgg
     1501 ccctgcaccg cagctacctg accccaggcg acagctccag cggatggacc gctggagctg
     1561 ctgcctacta cgtgggctac ctgcagcccc gcaccttcct gctgaagtac aacgagaatg
     1621 gcaccatcac cgacgccgtg gattgcgccc tggatccact gtccgagaca aagtgcaccc
     1681 tgaagagctt caccgtggag aagggcatct accagacctc caatttccgc gtgcagccaa
     1741 ccgagagcat cgtgcgcttc cccaatatca ccaacctgtg cccattcggc gaggtgttca
     1801 acgctaccag gttcgccagc gtgtacgctt ggaatcgcaa gcgcatctcc aactgcgtgg
     1861 ccgactacag cgtgctgtac aactccgcca gcttctccac cttcaagtgc tacggcgtgt
     1921 cccccaccaa gctgaatgat ctgtgcttca ccaacgtgta cgccgatagc ttcgtgatca
     1981 ggggcgacga ggtgcgccag atcgctccag gacagaccgg caagatcgct gactacaatt
     2041 acaagctgcc cgacgatttc accggctgcg tgatcgcctg gaactccaac aatctggata
     2101 gcaaagtggg cggcaactac aattacctgt accgcctgtt ccgcaagtcc aatctgaagc
     2161 cattcgagcg cgacatctcc accgagatct accaggctgg aagcacccca tgcaatggag
     2221 tggagggctt caactgctac ttccccctgc agagctacgg cttccagcca accaacggag
     2281 tgggatacca gccatacagg gtggtggtgc tgtccttcga gctgctgcac gctccagcta
     2341 ccgtgtgcgg accaaagaag agcaccaatc tggtgaagaa caagtgcgtg aacttcaatt
     2401 tcaacggcct gaccggaacc ggcgtgctga ccgagtccaa caagaagttc ctgccattcc
     2461 agcagttcgg aagggacatc gctgatacca ccgacgccgt gcgcgaccca cagaccctgg
     2521 agatcctgga tatcacccca tgctccttcg gcggcgtgag cgtgatcacc ccaggaacca
     2581 ataccagcaa ccaggtggcc gtgctgtacc agggcgtgaa ttgcaccgag gtgccagtgg
     2641 ctatccacgc tgatcagctg accccaacct ggcgcgtgta cagcaccgga tccaacgtgt
     2701 tccagacccg cgccggatgc ctgatcggag ctgagcacgt gaacaattcc tacgagtgcg
     2761 acatcccaat cggagctgga atctgcgcca gctaccagac ccagaccaac tccccaagga
     2821 gggctcgcag cgtggccagc cagtccatca tcgcctacac catgtccctg ggcgccgaga
     2881 atagcgtggc ctacagcaac aattccatcg ccatcccaac caacttcacc atctccgtga
     2941 ccaccgagat cctgcccgtg tccatgacca agaccagcgt ggactgcacc atgtacatct
     3001 gcggcgattc caccgagtgc agcaacctgc tgctgcagta cggcagcttc tgcacccagc
     3061 tgaatcgcgc cctgaccgga atcgctgtgg agcaggataa gaacacccag gaggtgttcg
     3121 cccaggtgaa gcagatctac aagacccccc caatcaagga cttcggcggc ttcaatttca
     3181 gccagatcct gcccgatcca agcaagccct ccaagcgcag cttcatcgag gacctgctgt
     3241 tcaacaaggt gaccctggcc gatgccggct tcatcaagca gtacggcgat tgcctgggcg
     3301 acatcgctgc ccgcgacctg atctgcgccc agaagttcaa tggcctgacc gtgctgccac
     3361 cactgctgac cgatgagatg atcgctcagt acacctccgc cctgctggcc ggaaccatca
     3421 ccagcggatg gaccttcggc gctggagccg ccctgcagat ccccttcgcc atgcagatgg
     3481 cctaccgctt caacggcatc ggcgtgaccc agaatgtgct gtacgagaac cagaagctga
     3541 tcgccaatca gttcaactcc gccatcggca agatccagga ctccctgtcc agcaccgcca
     3601 gcgccctggg caagctgcag gatgtggtga atcagaacgc ccaggccctg aataccctgg
     3661 tgaagcagct gtccagcaac ttcggcgcca tctccagcgt gctgaatgat atcctgagcc
     3721 gcctggacaa ggtggaggct gaggtgcaga tcgataggct gatcaccggc cgcctgcagt
     3781 ccctgcagac ctacgtgacc cagcagctga tcagggctgc tgagatcagg gccagcgcca
     3841 atctggctgc taccaagatg tccgagtgcg tgctgggaca gagcaagagg gtggacttct
     3901 gcggcaaggg ctaccacctg atgtccttcc cacagagcgc cccacacgga gtggtgttcc
     3961 tgcacgtgac ctacgtgcca gcccaggaga agaacttcac caccgctcca gctatctgcc
     4021 acgatggcaa ggctcacttc ccacgcgagg gcgtgttcgt gtccaacggc acccactggt
     4081 tcgtgaccca gcgcaatttc tacgagcccc agatcatcac caccgacaat accttcgtga
     4141 gcggcaactg cgacgtggtc atcggaatcg tgaacaatac cgtgtacgat cccctgcagc
     4201 cagagctgga ctccttcaag gaggagctgg ataagtactt caagaatcac accagccccg
     4261 acgtggatct gggcgacatc tccggcatca atgccagcgt ggtgaacatc cagaaggaga
     4321 tcgaccgcct gaacgaggtg gccaagaatc tgaacgagtc cctgatcgat ctgcaggagc
     4381 tgggcaagta cgagcagtac atcaagtggc catggtacat ctggctgggc ttcatcgccg
     4441 gcctgatcgc catcgtgatg gtgaccatca tgctgtgctg catgacctcc tgctgcagct
     4501 gcctgaaggg ctgctgctcc tgcggcagct gctgcaagtt cgatgaggac gatagcgagc
     4561 ccgtgctgaa gggcgtcaaa ctgcactata catagctcga gtctagaggg cccgtttaaa
     4621 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4681 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4741 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4801 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4861 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4981 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5041 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5101 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5221 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5281 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5341 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5401 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5521 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5581 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5641 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5701 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5761 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5821 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5881 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5941 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6001 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6061 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6121 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6181 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6241 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6301 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6361 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6421 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6481 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6541 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6601 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6661 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6841 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6901 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6961 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7021 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7081 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7141 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7201 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7261 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7321 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7381 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7441 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7501 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7561 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7621 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7681 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7741 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7801 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7861 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7921 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7981 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8041 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8101 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8161 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8221 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8281 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8341 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8401 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8461 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8521 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8581 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8641 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8701 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8761 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8821 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8881 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8941 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9001 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     9061 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     9121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaa
//
