LOCUS       sequence-34825-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Lin CH, Jackson AL, Guo J, Linsley PS, Eisenman RN
  TITLE     Myc-regulated microRNAs attenuate embryonic stem cell 
            differentiation.
  JOURNAL   EMBO J. 2009 Oct 21. 28(20):3157-70.
  PUBMED    19745813
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2009 Oct 21. 28(20):3157-70."
COMMENT     Sequence Label: Lenti-sh1368 knockdown c-myc
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..879)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(196..215)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     806..825
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 aagtatcttt aaattttgta tccagaggtt gattatcgat aagcttgata tcgaattaat
       61 tcgatgcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
      121 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
      181 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
      241 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
      301 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
      361 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
      421 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
      481 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
      541 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
      601 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
      661 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
      721 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
      781 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
      841 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg gtattatcat cgtgtttttc
      901 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg ttttttaacc tcgactaaac
      961 ac
//
