LOCUS       sequence-348252-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (Epsilon: S13I, W152C, L452R, D614G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-Epsilon
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..41)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        213..592
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        593..796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     747..767
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     841..860
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        841..859
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4738..4755)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4744..4968
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5014..5442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5101..5120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5311..5332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5451..5471)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5456..5785
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5636..5771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5698..5717
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5852..6646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5906..5925)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6516..6535
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6820..6941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6857..6876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6911..6930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6990..7006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6990..7006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7003..7025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7014..7030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7038..7044,7045..7062,7063..7068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7083..7104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7221..7238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7392..7980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7472..7491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8151..8942,8943..9011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8774..8793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9012..9116)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
       61 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
      121 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      181 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      241 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      301 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      361 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      421 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      481 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      541 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      601 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      661 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      721 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      781 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
      841 taatacgact cactataggg agacccaagc tggctagcat gtttgtcttc ctggtcctgc
      901 tgcctctggt ctcgattcag tgcgtgaacc tgactactag aacccagctg cctcctgcct
      961 atactaactc cttcacccgc ggcgtgtact acccagacaa ggtgttccgc agctccgtgc
     1021 tgcactccac ccaggatctg ttcctgccct tcttcagcaa cgtgacctgg ttccacgcca
     1081 tccacgtgag cggcaccaat ggcaccaagc ggttcgacaa tcccgtgctg ccattcaacg
     1141 atggcgtgta cttcgcctcc accgagaaga gcaacatcat ccgcggctgg atcttcggca
     1201 ccaccctgga ctccaagacc cagagcctgc tgatcgtgaa caatgccacc aacgtggtca
     1261 tcaaggtgtg cgagttccag ttctgcaatg atccattcct gggcgtgtac taccacaaga
     1321 acaataagtc ctgtatggag agcgagttcc gcgtgtacag ctccgccaac aattgcacct
     1381 tcgagtacgt gtcccagccc ttcctgatgg acctggaggg caagcagggc aatttcaaga
     1441 acctgcgcga gttcgtgttc aagaatatcg atggctactt caagatctac tccaagcaca
     1501 cccccatcaa cctggtgcgc gacctgccac agggcttcag cgccctggag ccactggtgg
     1561 atctgccaat cggcatcaac atcaccaggt tccagaccct gctggccctg caccgcagct
     1621 acctgacccc aggcgacagc tccagcggat ggaccgctgg agctgctgcc tactacgtgg
     1681 gctacctgca gccccgcacc ttcctgctga agtacaacga gaatggcacc atcaccgacg
     1741 ccgtggattg cgccctggat ccactgtccg agacaaagtg caccctgaag agcttcaccg
     1801 tggagaaggg catctaccag acctccaatt tccgcgtgca gccaaccgag agcatcgtgc
     1861 gcttccccaa tatcaccaac ctgtgcccat tcggcgaggt gttcaacgct accaggttcg
     1921 ccagcgtgta cgcttggaat cgcaagcgca tctccaactg cgtggccgac tacagcgtgc
     1981 tgtacaactc cgccagcttc tccaccttca agtgctacgg cgtgtccccc accaagctga
     2041 atgatctgtg cttcaccaac gtgtacgccg atagcttcgt gatcaggggc gacgaggtgc
     2101 gccagatcgc tccaggacag accggcaaga tcgctgacta caattacaag ctgcccgacg
     2161 atttcaccgg ctgcgtgatc gcctggaact ccaacaatct ggatagcaaa gtgggcggca
     2221 actacaatta ccggtaccgc ctgttccgca agtccaatct gaagccattc gagcgcgaca
     2281 tctccaccga gatctaccag gctggaagca ccccatgcaa tggagtggag ggcttcaact
     2341 gctacttccc cctgcagagc tacggcttcc agccaaccaa cggagtggga taccagccat
     2401 acagggtggt ggtgctgtcc ttcgagctgc tgcacgctcc agctaccgtg tgcggaccaa
     2461 agaagagcac caatctggtg aagaacaagt gcgtgaactt caatttcaac ggcctgaccg
     2521 gaaccggcgt gctgaccgag tccaacaaga agttcctgcc attccagcag ttcggaaggg
     2581 acatcgctga taccaccgac gccgtgcgcg acccacagac cctggagatc ctggatatca
     2641 ccccatgctc cttcggcggc gtgagcgtga tcaccccagg aaccaatacc agcaaccagg
     2701 tggccgtgct gtaccagggc gtgaattgca ccgaggtgcc agtggctatc cacgctgatc
     2761 agctgacccc aacctggcgc gtgtacagca ccggatccaa cgtgttccag acccgcgccg
     2821 gatgcctgat cggagctgag cacgtgaaca attcctacga gtgcgacatc ccaatcggag
     2881 ctggaatctg cgccagctac cagacccaga ccaactcccc aaggagggct cgcagcgtgg
     2941 ccagccagtc catcatcgcc tacaccatgt ccctgggcgc cgagaatagc gtggcctaca
     3001 gcaacaattc catcgccatc ccaaccaact tcaccatctc cgtgaccacc gagatcctgc
     3061 ccgtgtccat gaccaagacc agcgtggact gcaccatgta catctgcggc gattccaccg
     3121 agtgcagcaa cctgctgctg cagtacggca gcttctgcac ccagctgaat cgcgccctga
     3181 ccggaatcgc tgtggagcag gataagaaca cccaggaggt gttcgcccag gtgaagcaga
     3241 tctacaagac ccccccaatc aaggacttcg gcggcttcaa tttcagccag atcctgcccg
     3301 atccaagcaa gccctccaag cgcagcttca tcgaggacct gctgttcaac aaggtgaccc
     3361 tggccgatgc cggcttcatc aagcagtacg gcgattgcct gggcgacatc gctgcccgcg
     3421 acctgatctg cgcccagaag ttcaatggcc tgaccgtgct gccaccactg ctgaccgatg
     3481 agatgatcgc tcagtacacc tccgccctgc tggccggaac catcaccagc ggatggacct
     3541 tcggcgctgg agccgccctg cagatcccct tcgccatgca gatggcctac cgcttcaacg
     3601 gcatcggcgt gacccagaat gtgctgtacg agaaccagaa gctgatcgcc aatcagttca
     3661 actccgccat cggcaagatc caggactccc tgtccagcac cgccagcgcc ctgggcaagc
     3721 tgcaggatgt ggtgaatcag aacgcccagg ccctgaatac cctggtgaag cagctgtcca
     3781 gcaacttcgg cgccatctcc agcgtgctga atgatatcct gagccgcctg gacaaggtgg
     3841 aggctgaggt gcagatcgat aggctgatca ccggccgcct gcagtccctg cagacctacg
     3901 tgacccagca gctgatcagg gctgctgaga tcagggccag cgccaatctg gctgctacca
     3961 agatgtccga gtgcgtgctg ggacagagca agagggtgga cttctgcggc aagggctacc
     4021 acctgatgtc cttcccacag agcgccccac acggagtggt gttcctgcac gtgacctacg
     4081 tgccagccca ggagaagaac ttcaccaccg ctccagctat ctgccacgat ggcaaggctc
     4141 acttcccacg cgagggcgtg ttcgtgtcca acggcaccca ctggttcgtg acccagcgca
     4201 atttctacga gccccagatc atcaccaccg acaatacctt cgtgagcggc aactgcgacg
     4261 tggtcatcgg aatcgtgaac aataccgtgt acgatcccct gcagccagag ctggactcct
     4321 tcaaggagga gctggataag tacttcaaga atcacaccag ccccgacgtg gatctgggcg
     4381 acatctccgg catcaatgcc agcgtggtga acatccagaa ggagatcgac cgcctgaacg
     4441 aggtggccaa gaatctgaac gagtccctga tcgatctgca ggagctgggc aagtacgagc
     4501 agtacatcaa gtggccatgg tacatctggc tgggcttcat cgccggcctg atcgccatcg
     4561 tgatggtgac catcatgctg tgctgcatga cctcctgctg cagctgcctg aagggctgct
     4621 gctcctgcgg cagctgctgc aagttcgatg aggacgatag cgagcccgtg ctgaagggcg
     4681 tcaaactgca ctatacatag ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     4741 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4801 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4861 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4921 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4981 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     5041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5461 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5521 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5581 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5701 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5761 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5821 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5881 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5941 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6001 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6061 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6121 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6181 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6241 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6301 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6361 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6421 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6481 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6541 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6601 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6661 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6721 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6781 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6961 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7021 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7081 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7141 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7201 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7261 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7321 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7381 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7561 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7801 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8101 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8221 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8281 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9121 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccga
//
