LOCUS       sequence-348258-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y,
            D614G, H655Y, T1027I, V1176F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-Gamma
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        502..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     656..676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     750..769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        750..768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4647..4664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4653..4877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4923..5351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5010..5029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5220..5241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5360..5380)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5365..5694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5545..5680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5607..5626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5761..6555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5815..5834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6425..6444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6729..6850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6766..6785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6820..6839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6899..6915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6899..6915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6912..6934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6923..6939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6947..6953,6954..6971,6972..6977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6992..7013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7130..7147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7301..7889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7381..7400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8060..8851,8852..8920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8683..8702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8921..9025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9100..9119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      121 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      181 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      601 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      661 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      721 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      781 ggctagcatg tttgtcttcc tggtcctgct gcctctggtc tcgtctcagt gcgtgaactt
      841 tactaataga acccagctgc cttctgccta tactaactcc ttcacccgcg gcgtgtacta
      901 cccagacaag gtgttccgca gctccgtgct gcactccacc caggatctgt tcctgccctt
      961 cttcagcaac gtgacctggt tccacgccat ccacgtgagc ggcaccaatg gcaccaagcg
     1021 gttcgacaat cccgtgctgc cattcaacga tggcgtgtac ttcgcctcca ccgagaagag
     1081 caacatcatc cgcggctgga tcttcggcac caccctggac tccaagaccc agagcctgct
     1141 gatcgtgaac aatgccacca acgtggtcat caaggtgtgc gagttccagt tctgcaatta
     1201 tccattcctg ggcgtgtact accacaagaa caataagtcc tggatggaga gcgagttccg
     1261 cgtgtacagc tccgccaaca attgcacctt cgagtacgtg tcccagccct tcctgatgga
     1321 cctggagggc aagcagggca atttcaagaa cctgagcgag ttcgtgttca agaatatcga
     1381 tggctacttc aagatctact ccaagcacac ccccatcaac ctggtgcgcg acctgccaca
     1441 gggcttcagc gccctggagc cactggtgga tctgccaatc ggcatcaaca tcaccaggtt
     1501 ccagaccctg ctggccctgc accgcagcta cctgacccca ggcgacagct ccagcggatg
     1561 gaccgctgga gctgctgcct actacgtggg ctacctgcag ccccgcacct tcctgctgaa
     1621 gtacaacgag aatggcacca tcaccgacgc cgtggattgc gccctggatc cactgtccga
     1681 gacaaagtgc accctgaaga gcttcaccgt ggagaagggc atctaccaga cctccaattt
     1741 ccgcgtgcag ccaaccgaga gcatcgtgcg cttccccaat atcaccaacc tgtgcccatt
     1801 cggcgaggtg ttcaacgcta ccaggttcgc cagcgtgtac gcttggaatc gcaagcgcat
     1861 ctccaactgc gtggccgact acagcgtgct gtacaactcc gccagcttct ccaccttcaa
     1921 gtgctacggc gtgtccccca ccaagctgaa tgatctgtgc ttcaccaacg tgtacgccga
     1981 tagcttcgtg atcaggggcg acgaggtgcg ccagatcgct ccaggacaga ccggcaccat
     2041 cgctgactac aattacaagc tgcccgacga tttcaccggc tgcgtgatcg cctggaactc
     2101 caacaatctg gatagcaaag tgggcggcaa ctacaattac ctgtaccgcc tgttccgcaa
     2161 gtccaatctg aagccattcg agcgcgacat ctccaccgag atctaccagg ctggaagcac
     2221 cccatgcaat ggagtgaagg gcttcaactg ctacttcccc ctgcagagct acggcttcca
     2281 gccaacctac ggagtgggat accagccata cagggtggtg gtgctgtcct tcgagctgct
     2341 gcacgctcca gctaccgtgt gcggaccaaa gaagagcacc aatctggtga agaacaagtg
     2401 cgtgaacttc aatttcaacg gcctgaccgg aaccggcgtg ctgaccgagt ccaacaagaa
     2461 gttcctgcca ttccagcagt tcggaaggga catcgctgat accaccgacg ccgtgcgcga
     2521 cccacagacc ctggagatcc tggatatcac cccatgctcc ttcggcggcg tgagcgtgat
     2581 caccccagga accaatacca gcaaccaggt ggccgtgctg taccagggcg tgaattgcac
     2641 cgaggtgcca gtggctatcc acgctgatca gctgacccca acctggcgcg tgtacagcac
     2701 cggatccaac gtgttccaga cccgcgccgg atgcctgatc ggagctgagt acgtgaacaa
     2761 ttcctacgag tgcgacatcc caatcggagc tggaatctgc gccagctacc agacccagac
     2821 caactcccca aggagggctc gcagcgtggc cagccagtcc atcatcgcct acaccatgtc
     2881 cctgggcgcc gagaatagcg tggcctacag caacaattcc atcgccatcc caaccaactt
     2941 caccatctcc gtgaccaccg agatcctgcc cgtgtccatg accaagacca gcgtggactg
     3001 caccatgtac atctgcggcg attccaccga gtgcagcaac ctgctgctgc agtacggcag
     3061 cttctgcacc cagctgaatc gcgccctgac cggaatcgct gtggagcagg ataagaacac
     3121 ccaggaggtg ttcgcccagg tgaagcagat ctacaagacc cccccaatca aggacttcgg
     3181 cggcttcaat ttcagccaga tcctgcccga tccaagcaag ccctccaagc gcagcttcat
     3241 cgaggacctg ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca agcagtacgg
     3301 cgattgcctg ggcgacatcg ctgcccgcga cctgatctgc gcccagaagt tcaatggcct
     3361 gaccgtgctg ccaccactgc tgaccgatga gatgatcgct cagtacacct ccgccctgct
     3421 ggccggaacc atcaccagcg gatggacctt cggcgctgga gccgccctgc agatcccctt
     3481 cgccatgcag atggcctacc gcttcaacgg catcggcgtg acccagaatg tgctgtacga
     3541 gaaccagaag ctgatcgcca atcagttcaa ctccgccatc ggcaagatcc aggactccct
     3601 gtccagcacc gccagcgccc tgggcaagct gcaggatgtg gtgaatcaga acgcccaggc
     3661 cctgaatacc ctggtgaagc agctgtccag caacttcggc gccatctcca gcgtgctgaa
     3721 tgatatcctg agccgcctgg acaaggtgga ggctgaggtg cagatcgata ggctgatcac
     3781 cggccgcctg cagtccctgc agacctacgt gacccagcag ctgatcaggg ctgctgagat
     3841 cagggccagc gccaatctgg ctgctatcaa gatgtccgag tgcgtgctgg gacagagcaa
     3901 gagggtggac ttctgcggca agggctacca cctgatgtcc ttcccacaga gcgccccaca
     3961 cggagtggtg ttcctgcacg tgacctacgt gccagcccag gagaagaact tcaccaccgc
     4021 tccagctatc tgccacgatg gcaaggctca cttcccacgc gagggcgtgt tcgtgtccaa
     4081 cggcacccac tggttcgtga cccagcgcaa tttctacgag ccccagatca tcaccaccga
     4141 caataccttc gtgagcggca actgcgacgt ggtcatcgga atcgtgaaca ataccgtgta
     4201 cgatcccctg cagccagagc tggactcctt caaggaggag ctggataagt acttcaagaa
     4261 tcacaccagc cccgacgtgg atctgggcga catctccggc atcaatgcca gctttgtgaa
     4321 catccagaag gagatcgacc gcctgaacga ggtggccaag aatctgaacg agtccctgat
     4381 cgatctgcag gagctgggca agtacgagca gtacatcaag tggccatggt acatctggct
     4441 gggcttcatc gccggcctga tcgccatcgt gatggtgacc atcatgctgt gctgcatgac
     4501 ctcctgctgc agctgcctga agggctgctg ctcctgcggc agctgctgca agttcgatga
     4561 ggacgatagc gagcccgtgc tgaagggcgt caaactgcac tatacatagc tcgagtctag
     4621 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4681 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4741 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4801 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4861 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4921 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5161 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5281 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5341 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5701 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5941 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6541 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6601 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6661 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6721 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6781 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6841 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6961 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9061 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9121 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgag
//
