LOCUS       sequence-348261-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (Beta: K417N, E484K, N501Y, D614G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-Beta
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..32)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        204..583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        584..787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     738..758
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     832..851
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        832..850
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4729..4746)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4735..4959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5005..5433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5092..5111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5302..5323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5442..5462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5447..5776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5627..5762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5689..5708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5843..6637
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5897..5916)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6507..6526
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6811..6932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6848..6867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6902..6921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6981..6997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6981..6997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6994..7016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7005..7021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7029..7035,7036..7053,7054..7059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7074..7095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7212..7229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7383..7971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7463..7482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8142..8933,8934..9002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8765..8784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9003..9107)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
       61 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
      121 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      181 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      601 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      661 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      721 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      781 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
      841 tcactatagg gagacccaag ctggctagca tgtttgtctt cctggtcctg ctgcctctgg
      901 tctcgtctca gtgcgtgaac ctgactacta gaacccagct gcctcctgcc tatactaact
      961 ccttcacccg cggcgtgtac tacccagaca aggtgttccg cagctccgtg ctgcactcca
     1021 cccaggatct gttcctgccc ttcttcagca acgtgacctg gttccacgcc atccacgtga
     1081 gcggcaccaa tggcaccaag cggttcgaca atcccgtgct gccattcaac gatggcgtgt
     1141 acttcgcctc caccgagaag agcaacatca tccgcggctg gatcttcggc accaccctgg
     1201 actccaagac ccagagcctg ctgatcgtga acaatgccac caacgtggtc atcaaggtgt
     1261 gcgagttcca gttctgcaat gatccattcc tgggcgtgta ctaccacaag aacaataagt
     1321 cctggatgga gagcgagttc cgcgtgtaca gctccgccaa caattgcacc ttcgagtacg
     1381 tgtcccagcc cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgcgcg
     1441 agttcgtgtt caagaatatc gatggctact tcaagatcta ctccaagcac acccccatca
     1501 acctggtgcg cgacctgcca cagggcttca gcgccctgga gccactggtg gatctgccaa
     1561 tcggcatcaa catcaccagg ttccagaccc tgctggccct gcaccgcagc tacctgaccc
     1621 caggcgacag ctccagcgga tggaccgctg gagctgctgc ctactacgtg ggctacctgc
     1681 agccccgcac cttcctgctg aagtacaacg agaatggcac catcaccgac gccgtggatt
     1741 gcgccctgga tccactgtcc gagacaaagt gcaccctgaa gagcttcacc gtggagaagg
     1801 gcatctacca gacctccaat ttccgcgtgc agccaaccga gagcatcgtg cgcttcccca
     1861 atatcaccaa cctgtgccca ttcggcgagg tgttcaacgc taccaggttc gccagcgtgt
     1921 acgcttggaa tcgcaagcgc atctccaact gcgtggccga ctacagcgtg ctgtacaact
     1981 ccgccagctt ctccaccttc aagtgctacg gcgtgtcccc caccaagctg aatgatctgt
     2041 gcttcaccaa cgtgtacgcc gatagcttcg tgatcagggg cgacgaggtg cgccagatcg
     2101 ctccaggaca gaccggcaac atcgctgact acaattacaa gctgcccgac gatttcaccg
     2161 gctgcgtgat cgcctggaac tccaacaatc tggatagcaa agtgggcggc aactacaatt
     2221 acctgtaccg cctgttccgc aagtccaatc tgaagccatt cgagcgcgac atctccaccg
     2281 agatctacca ggctggaagc accccatgca atggagtgaa gggcttcaac tgctacttcc
     2341 ccctgcagag ctacggcttc cagccaacct acggagtggg ataccagcca tacagggtgg
     2401 tggtgctgtc cttcgagctg ctgcacgctc cagctaccgt gtgcggacca aagaagagca
     2461 ccaatctggt gaagaacaag tgcgtgaact tcaatttcaa cggcctgacc ggaaccggcg
     2521 tgctgaccga gtccaacaag aagttcctgc cattccagca gttcggaagg gacatcgctg
     2581 ataccaccga cgccgtgcgc gacccacaga ccctggagat cctggatatc accccatgct
     2641 ccttcggcgg cgtgagcgtg atcaccccag gaaccaatac cagcaaccag gtggccgtgc
     2701 tgtaccaggg cgtgaattgc accgaggtgc cagtggctat ccacgctgat cagctgaccc
     2761 caacctggcg cgtgtacagc accggatcca acgtgttcca gacccgcgcc ggatgcctga
     2821 tcggagctga gcacgtgaac aattcctacg agtgcgacat cccaatcgga gctggaatct
     2881 gcgccagcta ccagacccag accaactccc caaggagggc tcgcagcgtg gccagccagt
     2941 ccatcatcgc ctacaccatg tccctgggcg ccgagaatag cgtggcctac agcaacaatt
     3001 ccatcgccat cccaaccaac ttcaccatct ccgtgaccac cgagatcctg cccgtgtcca
     3061 tgaccaagac cagcgtggac tgcaccatgt acatctgcgg cgattccacc gagtgcagca
     3121 acctgctgct gcagtacggc agcttctgca cccagctgaa tcgcgccctg accggaatcg
     3181 ctgtggagca ggataagaac acccaggagg tgttcgccca ggtgaagcag atctacaaga
     3241 cccccccaat caaggacttc ggcggcttca atttcagcca gatcctgccc gatccaagca
     3301 agccctccaa gcgcagcttc atcgaggacc tgctgttcaa caaggtgacc ctggccgatg
     3361 ccggcttcat caagcagtac ggcgattgcc tgggcgacat cgctgcccgc gacctgatct
     3421 gcgcccagaa gttcaatggc ctgaccgtgc tgccaccact gctgaccgat gagatgatcg
     3481 ctcagtacac ctccgccctg ctggccggaa ccatcaccag cggatggacc ttcggcgctg
     3541 gagccgccct gcagatcccc ttcgccatgc agatggccta ccgcttcaac ggcatcggcg
     3601 tgacccagaa tgtgctgtac gagaaccaga agctgatcgc caatcagttc aactccgcca
     3661 tcggcaagat ccaggactcc ctgtccagca ccgccagcgc cctgggcaag ctgcaggatg
     3721 tggtgaatca gaacgcccag gccctgaata ccctggtgaa gcagctgtcc agcaacttcg
     3781 gcgccatctc cagcgtgctg aatgatatcc tgagccgcct ggacaaggtg gaggctgagg
     3841 tgcagatcga taggctgatc accggccgcc tgcagtccct gcagacctac gtgacccagc
     3901 agctgatcag ggctgctgag atcagggcca gcgccaatct ggctgctacc aagatgtccg
     3961 agtgcgtgct gggacagagc aagagggtgg acttctgcgg caagggctac cacctgatgt
     4021 ccttcccaca gagcgcccca cacggagtgg tgttcctgca cgtgacctac gtgccagccc
     4081 aggagaagaa cttcaccacc gctccagcta tctgccacga tggcaaggct cacttcccac
     4141 gcgagggcgt gttcgtgtcc aacggcaccc actggttcgt gacccagcgc aatttctacg
     4201 agccccagat catcaccacc gacaatacct tcgtgagcgg caactgcgac gtggtcatcg
     4261 gaatcgtgaa caataccgtg tacgatcccc tgcagccaga gctggactcc ttcaaggagg
     4321 agctggataa gtacttcaag aatcacacca gccccgacgt ggatctgggc gacatctccg
     4381 gcatcaatgc cagcgtggtg aacatccaga aggagatcga ccgcctgaac gaggtggcca
     4441 agaatctgaa cgagtccctg atcgatctgc aggagctggg caagtacgag cagtacatca
     4501 agtggccatg gtacatctgg ctgggcttca tcgccggcct gatcgccatc gtgatggtga
     4561 ccatcatgct gtgctgcatg acctcctgct gcagctgcct gaagggctgc tgctcctgcg
     4621 gcagctgctg caagttcgat gaggacgata gcgagcccgt gctgaagggc gtcaaactgc
     4681 actatacata gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4741 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4801 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4861 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4921 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4981 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5221 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5341 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5401 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5461 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5521 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5581 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5641 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5701 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5761 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5821 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5881 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5941 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6001 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6061 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6121 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6181 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6241 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6301 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6361 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6421 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6481 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6541 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6601 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6661 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6721 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6781 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6841 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6901 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6961 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7021 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7801 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8281 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9001 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9061 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9121 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatg
//
