LOCUS       sequence-348264-        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient mammalian expression of codon optimized SARS-CoV-2 Spike 
            protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I,
            S982A, D1118H).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Deng X, Garcia-Knight MA, Khalid MM, Servellita V, Wang C, Morris 
            MK, Sotomayor-Gonzalez A, Glasner DR, Reyes KR, Gliwa AS, Reddy NP, 
            Sanchez San Martin C, Federman S, Cheng J, Balcerek J, Taylor J, 
            Streithorst JA, Miller S, Sreekumar B, Chen PY, Schulze-Gahmen U, 
            Taha TY, Hayashi JM, Simoneau CR, Kumar GR, McMahon S, Lidsky PV, 
            Xiao Y, Hemarajata P, Green NM, Espinosa A, Kath C, Haw M, Bell J, 
            Hacker JK, Hanson C, Wadford DA, Anaya C, Ferguson D, Frankino PA, 
            Shivram H, Lareau LF, Wyman SK, Ott M, Andino R, Chiu CY
  TITLE     Transmission, infectivity, and neutralization of a spike L452R 
            SARS-CoV-2 variant.
  JOURNAL   Cell. 2021 Jun 24;184(13):3426-3437.e8. doi: 
            10.1016/j.cell.2021.04.025. Epub 2021 Apr 20.
  PUBMED    33991487
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.04.025"; journalName: "Cell"; date: 
            "2021-06-24- 24"; volume: "184"; issue: "13"; pages: "3426-3437.e8"
COMMENT     Sequence Label: CoV2-Spike-D614G-Alpha
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     781..800
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        781..799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4669..4686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4675..4899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4945..5373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5032..5051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5242..5263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5382..5402)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5387..5716
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5567..5702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5629..5648
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5783..6577
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5837..5856)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6447..6466
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6751..6872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6788..6807)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6842..6861
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6921..6937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6921..6937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6934..6956)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6945..6961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6969..6975,6976..6993,6994..6999))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7014..7035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7152..7169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7323..7911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7403..7422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8082..8873,8874..8942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8705..8724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8943..9047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9122..9141)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
       61 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      121 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
      781 taatacgact cactataggg agacccaagc tggctagcat gtttgtcttc ctggtcctgc
      841 tgcctctggt ctcgtctcag tgcgtgaacc tgactactag aacccagctg cctcctgcct
      901 atactaactc cttcacccgc ggcgtgtact acccagacaa ggtgttccgc agctccgtgc
      961 tgcactccac ccaggatctg ttcctgccct tcttcagcaa cgtgacctgg ttccacgcca
     1021 tcagcggcac caatggcacc aagcggttcg acaatcccgt gctgccattc aacgatggcg
     1081 tgtacttcgc ctccaccgag aagagcaaca tcatccgcgg ctggatcttc ggcaccaccc
     1141 tggactccaa gacccagagc ctgctgatcg tgaacaatgc caccaacgtg gtcatcaagg
     1201 tgtgcgagtt ccagttctgc aatgatccat tcctgggcgt gtaccacaag aacaataagt
     1261 cctggatgga gagcgagttc cgcgtgtaca gctccgccaa caattgcacc ttcgagtacg
     1321 tgtcccagcc cttcctgatg gacctggagg gcaagcaggg caatttcaag aacctgcgcg
     1381 agttcgtgtt caagaatatc gatggctact tcaagatcta ctccaagcac acccccatca
     1441 acctggtgcg cgacctgcca cagggcttca gcgccctgga gccactggtg gatctgccaa
     1501 tcggcatcaa catcaccagg ttccagaccc tgctggccct gcaccgcagc tacctgaccc
     1561 caggcgacag ctccagcgga tggaccgctg gagctgctgc ctactacgtg ggctacctgc
     1621 agccccgcac cttcctgctg aagtacaacg agaatggcac catcaccgac gccgtggatt
     1681 gcgccctgga tccactgtcc gagacaaagt gcaccctgaa gagcttcacc gtggagaagg
     1741 gcatctacca gacctccaat ttccgcgtgc agccaaccga gagcatcgtg cgcttcccca
     1801 atatcaccaa cctgtgccca ttcggcgagg tgttcaacgc taccaggttc gccagcgtgt
     1861 acgcttggaa tcgcaagcgc atctccaact gcgtggccga ctacagcgtg ctgtacaact
     1921 ccgccagctt ctccaccttc aagtgctacg gcgtgtcccc caccaagctg aatgatctgt
     1981 gcttcaccaa cgtgtacgcc gatagcttcg tgatcagggg cgacgaggtg cgccagatcg
     2041 ctccaggaca gaccggcaag atcgctgact acaattacaa gctgcccgac gatttcaccg
     2101 gctgcgtgat cgcctggaac tccaacaatc tggatagcaa agtgggcggc aactacaatt
     2161 acctgtaccg cctgttccgc aagtccaatc tgaagccatt cgagcgcgac atctccaccg
     2221 agatctacca ggctggaagc accccatgca atggagtgga gggcttcaac tgctacttcc
     2281 ccctgcagag ctacggcttc cagccaacct acggagtggg ataccagcca tacagggtgg
     2341 tggtgctgtc cttcgagctg ctgcacgctc cagctaccgt gtgcggacca aagaagagca
     2401 ccaatctggt gaagaacaag tgcgtgaact tcaatttcaa cggcctgacc ggaaccggcg
     2461 tgctgaccga gtccaacaag aagttcctgc cattccagca gttcggaagg gacatcgatg
     2521 ataccaccga cgccgtgcgc gacccacaga ccctggagat cctggatatc accccatgct
     2581 ccttcggcgg cgtgagcgtg atcaccccag gaaccaatac cagcaaccag gtggccgtgc
     2641 tgtaccaggg cgtgaattgc accgaggtgc cagtggctat ccacgctgat cagctgaccc
     2701 caacctggcg cgtgtacagc accggatcca acgtgttcca gacccgcgcc ggatgcctga
     2761 tcggagctga gcacgtgaac aattcctacg agtgcgacat cccaatcgga gctggaatct
     2821 gcgccagcta ccagacccag accaactccc acaggagggc tcgcagcgtg gccagccagt
     2881 ccatcatcgc ctacaccatg tccctgggcg ccgagaatag cgtggcctac agcaacaatt
     2941 ccatcgccat cccaatcaac ttcaccatct ccgtgaccac cgagatcctg cccgtgtcca
     3001 tgaccaagac cagcgtggac tgcaccatgt acatctgcgg cgattccacc gagtgcagca
     3061 acctgctgct gcagtacggc agcttctgca cccagctgaa tcgcgccctg accggaatcg
     3121 ctgtggagca ggataagaac acccaggagg tgttcgccca ggtgaagcag atctacaaga
     3181 cccccccaat caaggacttc ggcggcttca atttcagcca gatcctgccc gatccaagca
     3241 agccctccaa gcgcagcttc atcgaggacc tgctgttcaa caaggtgacc ctggccgatg
     3301 ccggcttcat caagcagtac ggcgattgcc tgggcgacat cgctgcccgc gacctgatct
     3361 gcgcccagaa gttcaatggc ctgaccgtgc tgccaccact gctgaccgat gagatgatcg
     3421 ctcagtacac ctccgccctg ctggccggaa ccatcaccag cggatggacc ttcggcgctg
     3481 gagccgccct gcagatcccc ttcgccatgc agatggccta ccgcttcaac ggcatcggcg
     3541 tgacccagaa tgtgctgtac gagaaccaga agctgatcgc caatcagttc aactccgcca
     3601 tcggcaagat ccaggactcc ctgtccagca ccgccagcgc cctgggcaag ctgcaggatg
     3661 tggtgaatca gaacgcccag gccctgaata ccctggtgaa gcagctgtcc agcaacttcg
     3721 gcgccatctc cagcgtgctg aatgatatcc tggctcgcct ggacaaggtg gaggctgagg
     3781 tgcagatcga taggctgatc accggccgcc tgcagtccct gcagacctac gtgacccagc
     3841 agctgatcag ggctgctgag atcagggcca gcgccaatct ggctgctacc aagatgtccg
     3901 agtgcgtgct gggacagagc aagagggtgg acttctgcgg caagggctac cacctgatgt
     3961 ccttcccaca gagcgcccca cacggagtgg tgttcctgca cgtgacctac gtgccagccc
     4021 aggagaagaa cttcaccacc gctccagcta tctgccacga tggcaaggct cacttcccac
     4081 gcgagggcgt gttcgtgtcc aacggcaccc actggttcgt gacccagcgc aatttctacg
     4141 agccccagat catcaccacc cacaatacct tcgtgagcgg caactgcgac gtggtcatcg
     4201 gaatcgtgaa caataccgtg tacgatcccc tgcagccaga gctggactcc ttcaaggagg
     4261 agctggataa gtacttcaag aatcacacca gccccgacgt ggatctgggc gacatctccg
     4321 gcatcaatgc cagcgtggtg aacatccaga aggagatcga ccgcctgaac gaggtggcca
     4381 agaatctgaa cgagtccctg atcgatctgc aggagctggg caagtacgag cagtacatca
     4441 agtggccatg gtacatctgg ctgggcttca tcgccggcct gatcgccatc gtgatggtga
     4501 ccatcatgct gtgctgcatg acctcctgct gcagctgcct gaagggctgc tgctcctgcg
     4561 gcagctgctg caagttcgat gaggacgata gcgagcccgt gctgaagggc gtcaaactgc
     4621 actatacata gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4861 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4921 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5281 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5341 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5401 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5461 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5521 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5581 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5641 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5701 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5761 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5821 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5881 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5941 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6001 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6061 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6121 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6181 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6241 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6301 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6361 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6421 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6481 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6541 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6601 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6661 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6721 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6781 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6841 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6901 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7141 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9061 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     9121 gtacaatctg ctctgatgcc gcatagttaa gccagtatct
//
