LOCUS       sequence-348280-        8351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to be used as DNA template for in-vitro RNA transcription of
            the BE3 base editor (C to T) by T3 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Jalil S, Keskinen T, Maldonado R, Sokka J, Trokovic R, Otonkoski T, 
            Wartiovaara K
  TITLE     Simultaneous high-efficiency base editing and reprogramming of 
            patient fibroblasts.
  JOURNAL   Stem Cell Reports. 2021 Nov 16. pii: S2213-6711(21)00552-X. doi: 
            10.1016/j.stemcr.2021.10.017.
  PUBMED    34822772
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Nov 16. pii: S2213-6711(21)00552-X. doi: 
            10.1016/j.stemcr.2021.10.017."
COMMENT     Sequence Label: pRN3P_T3_BE3_IVT
FEATURES             Location/Qualifiers
     source          1..8351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           88..130
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             169..855
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             904..5004
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLXDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5017..5265
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5278..5298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     3'UTR           5335..5460
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(5583..5602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5584..5602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5609..5626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5609..5625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5618..5640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5767..6222)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5904..5925)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6116..6135
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6249..6353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6354..6422,6423..7214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6572..6591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7385..7973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7874..7893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8127..8144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8261..8282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8297..8302,8303..8320,8321..8327)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8335..8351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8340..8351,1..11)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
       61 agggaacaaa agctggagct aagcttgctt gttctttttg cagaagctca gaataaacgc
      121 tcaactttgg cagatctgaa ttcctgcagc ccgggatcca cagccaccat gagctcagag
      181 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
      241 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
      301 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
      361 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
      421 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
      481 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
      541 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
      601 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
      661 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
      721 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
      781 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
      841 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
      901 agtgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
      961 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     1021 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     1081 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     1141 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     1201 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1261 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1321 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1381 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1441 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1501 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1561 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
     1621 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1681 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1741 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1801 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1861 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1921 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1981 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     2041 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     2101 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     2161 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     2221 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     2281 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     2341 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     2401 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2461 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2521 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2581 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2641 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2701 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2761 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2821 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2881 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2941 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     3001 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     3061 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     3121 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     3181 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     3241 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacaccca
     3301 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     3361 gatatgtacg tggaccagga actggacatc aaccggctgt ycgactacga tgtggaccat
     3421 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3481 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3541 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3601 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3661 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3721 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3781 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3841 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3901 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3961 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     4021 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     4081 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     4141 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     4201 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     4261 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     4321 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     4381 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4441 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4501 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4561 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4621 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4681 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4741 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4801 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4861 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4921 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4981 gacctgtctc agctgggagg cgattcaggc ggatctacta atctgtcaga tattattgaa
     5041 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     5101 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     5161 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     5221 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     5281 aagaagaaga ggaaagtcta agtaggtgag cggccgcgga tctggttacc actaaaccag
     5341 cctcaagaac acccgaatgg agtctctaag ctacataata ccaacttaca ctttacaaaa
     5401 tgttgtcccc caaaatgtag ccattcgtat ctgctcctaa taaaaagaaa gtttcttcac
     5461 attctaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5521 aaaaaaaaaa aaamcccccc cccccccccc ctgcagggcc aagtcggccg gtacccaatt
     5581 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     5641 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5701 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5761 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     5821 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     5881 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     5941 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6001 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6061 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6121 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6181 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     6241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7501 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7741 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7801 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7861 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7921 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7981 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8041 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8101 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8161 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8221 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8281 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8341 gcggataaca a
//
