LOCUS       sequence-348287-        6808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse dynein regulatory complex subunit 3 
            (Drc3) tagged with PA at C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, 
            Shiba K, Inaba K, Ikawa M
  TITLE     Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is 
            required for sperm flagellum formation and male fertility in mice.
  JOURNAL   PLoS Genet. 2020 Jan 21;16(1):e1008585. doi: 
            10.1371/journal.pgen.1008585. eCollection 2020 Jan.
  PUBMED    31961863
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2020-01-21"; volume: "16(1):e1008585. doi"; pages: "
            10.1371/journal.pgen.1008585. eCollection 2020 Jan"
COMMENT     Sequence Label: pCAG-mDrc3-PA
FEATURES             Location/Qualifiers
     source          1..6808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          674..1688
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1614..1636
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1696..1715
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3488..3507)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3553..3608
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3607..3626)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3963..3982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3964..3982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3992..4009)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3992..4008)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4001..4023)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4150..4605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4237..4256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4447..4468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4631..4735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4736..4804,4805..5596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4954..4973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5767..6355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6256..6275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6509..6526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6643..6664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6679..6684,6685..6702,6703..6709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6717..6733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6722..6744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6741..6757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6741..6757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6776..6796
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6778..6796
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggsgc gcgccrggsg gggsggggsg
      541 gggsgrgggg gggggsgggg sgrggcggag aggtgcggcg gcagccaatc agagcggcgc
      601 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      661 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      721 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
      781 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
      841 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
      901 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
      961 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1021 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1081 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1141 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1201 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1261 gccgggcggg gcggggccgc ctcgggccgg ggrgggctcg ggggaggggc gcggcggccc
     1321 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1381 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1441 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     1501 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1561 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1621 cttctggcgt gtgaccggcg gctctagcct ctgctaacca tgttcatgcc ttcttctttt
     1681 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaatcta
     1741 gaggatccgc cgccatgagc cggccatatg actcgatgga gccaaaagta atggatgatg
     1801 acatgctcaa agcagctgtc ggggagcaag ggccccagga agaagctggg cagctagcca
     1861 agcaggaggg catcctcttc aaagatgtcc tgtccctgca gctggacttc cagaacatcc
     1921 tccgcatcga taacctctgg cagtttgaga acctgaagaa gctgcaactg aacaacaaca
     1981 tcatcgagcg catcgagggc ctgacgaacc tcatacacct cgtctggctg gatttgtcct
     2041 ttaacaacat cgaggccatc gagggcctgg ataccctggt caacctggag gacctgagct
     2101 tgtccaataa ccggatctcc aaggtggact ctctagatgc cctggtgaag ctgcaggtgc
     2161 tgtcactggg caacaaccag attagcaaca tgatgaatat catctacctg cgccggttcc
     2221 cgtgcctgcg aacactcagt ctcgctggga atcccgtttc tgaggcagag gagtacaaga
     2281 tgttcatcta tgcctatctt tctgacctcg tgtacttgga cttccggcgc gttgatgagc
     2341 agatgagaga gatggcgaag atgaaacatc agtacagcat tgacgagctg aagcaccgcg
     2401 aggcacagct gcagatgaag ctggaggagg agcaggccaa gcaggagaag ctggaggaac
     2461 acaagatggc gtttgttgag cacctgaacg gccccttcct gtttgacagc atgtattccg
     2521 aggatgtgga gggcaacaag ctgtcctacc tgcctggtgt tagagagctc cttgaagcct
     2581 acaaggacaa gtttgtcatc atctgcctga acatttttga gtatggcctg aatcagcaag
     2641 aaaaacggaa ggtagagtta gacactttca atgagtgcat acaggaggcc atcctggaaa
     2701 accaggacca gggcaagctc aaggttgcca agtttgagga aaagcacttg ctgaacttaa
     2761 atgccatccg ggaagagact gatctgtcca acatcgagaa gaagctgacg gagtgcactg
     2821 agagcatcgg tgagctgttc aacacactca tgatcctgga gatgcagctg gtggaacagc
     2881 tggaggagac cataaacata tttgaaagaa atatcactga cttggtggga ctatttattg
     2941 aaaatgtcca aagtctgatt gctcagtgcc gggacctgga gaaccatcac catgagaagc
     3001 tcctggagat cgccatcaac accctggaga agatcctcaa gggcgagatg gacgaggact
     3061 tacccgatga cgtgcgggcg ctttttgttg acaaagacac aattgttaat gccgttgggg
     3121 catcacatga catccacctc ctgaagattg acaaccgaga agatgagctg gtgactggaa
     3181 tcaactcttg gtgtgcacat ctagtagaca agattcacaa agatgagatc atgaggaacc
     3241 gtaagcgagt gaaggagatc aatcaattcg tcgaccacat gcagagtgaa ctggacaacc
     3301 tggaatgtgg agacattata gatgaattcg atatcaagct tgtcgacgcg gccctcgagg
     3361 ctagcggcgt tgccatgcca ggtgccgaag atgatgtggt gtaaatcgag actcctcagg
     3421 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     3481 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3541 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3601 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3661 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3721 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3781 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3841 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3901 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cttaattaag gtacccaatt
     3961 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4021 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4081 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4141 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4201 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4261 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4321 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4381 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4441 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4501 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4561 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4621 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4681 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4741 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4801 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4861 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4921 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4981 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5041 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5101 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5161 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5221 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5281 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5341 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5401 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5461 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5521 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5581 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5641 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5701 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5881 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6541 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6601 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6661 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6721 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     6781 taaccctcac taaagggaac aaaagctg
//
