LOCUS       sequence-348288-        6728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse t-complex-associated testis expressed 1 
            (Tcte1) tagged with PA at C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, 
            Shiba K, Inaba K, Ikawa M
  TITLE     Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is 
            required for sperm flagellum formation and male fertility in mice.
  JOURNAL   PLoS Genet. 2020 Jan 21;16(1):e1008585. doi: 
            10.1371/journal.pgen.1008585. eCollection 2020 Jan.
  PUBMED    31961863
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2020-01-21"; volume: "16(1):e1008585. doi"; pages: "
            10.1371/journal.pgen.1008585. eCollection 2020 Jan"
COMMENT     Sequence Label: pCAG-mTcte1-PA
FEATURES             Location/Qualifiers
     source          1..6728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..674
                     /label=chicken beta-actin promoter
     intron          675..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1615..1637
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3408..3427)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3473..3528
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3527..3546)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3883..3902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3884..3902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3912..3929)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3912..3928)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3921..3943)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4070..4525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4157..4176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4367..4388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4551..4655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4656..4724,4725..5516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4874..4893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5687..6275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6176..6195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6429..6446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6563..6584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6599..6604,6605..6622,6623..6629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6637..6653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6642..6664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6661..6677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6661..6677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6696..6716
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6698..6716
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggnsg cgcgccrggc ggggsggggs
      541 ggggsgrggg gsggggsggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
      601 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      661 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      721 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      781 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      841 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      901 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      961 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1021 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1081 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1141 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1201 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1261 tgccgggcgg ggcggggccg cctcgggccg gggrgggctc gggggagggg cgcggcggcc
     1321 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1381 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1501 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1561 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1621 gcttctggcg tgtgaccggc ggctctagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaatct
     1741 agaggatccg ccgccatgca ggaaacccca tcagtaccgt ccaactcttc gtctcacagc
     1801 cagtcggttc tcaccatcca gcgccaggtc tctgccttgg gcagctccag cacaggaccc
     1861 acgtccttga agaccagcag cacccctacc ccaggccagc tgaagaccaa ggtcccaaat
     1921 gtccgtcgca tgcgcaggat catctccgag gatgcagagt ggtcactggc catcgtacct
     1981 ctccttacag aactctgcat tcaacatatc gtcaagaact tccagaacaa ccctatcctg
     2041 aagcagctgc cgctagagca ccaaaagaag gtcttgagca acctgccccc cgagctgcct
     2101 ttgacggtga ccgctaactt gattgacgat gagaattact ggcaccgctg ctgcatcaag
     2161 cgctggtccg tgtgccacgt gtccaggcac ggcggcagct ggaagcgtat gttcttcgag
     2221 agacacctgg agaacctgct gaagctattc atcccgggga ccacggaccc caacgtgatc
     2281 ctggacctgc tgcccctctg caggaactac gtgcgccgca ttcatgtgga ccagttcctc
     2341 ccacctgtgc ggatgccgac cccgctccag ggagaggaac agtcggactc tggcagtgag
     2401 ggcgagggga gcgagccaga gaaggaccac taccagctgc agacgctggt gggtggcctc
     2461 aaacacctgg aggagctgga cctggtgtac ggcgtcaaag actgtggcat gaattttgag
     2521 tggaacctct ttctcttcac ctaccgagac tgctactctc tggcagccac catcaaagct
     2581 tgccacaccc tcaagatctt caagctgacc cgaagtaagg tggacgacga taaggcgcgc
     2641 atcctaatcc gtagcctgct ggaccaccca gccctggagg agctggactt atcccacaac
     2701 ctcatcggag accgtggtgc acgagcagcg gctaagctgc tgagccacag tcgcctgaga
     2761 gtgctcaacc tggccaacaa tcaactgcag gcgcctggcg cacagtcact ggctcacgca
     2821 ctggcacaca acaccaacct ggtcttcctc aacctccgcc tcaactgtat cgaggacgag
     2881 ggtggccaag caatcgccca cgccctggag accaacaagt gcctctccgt gctgcacctt
     2941 ggtggcaaca agctgtctga gcccacagcc acactcctct cccagatgct cactgtcaac
     3001 actacgctcg tcagcctcaa cctatcctgc aaccacattg ggcaggatgg tgggaagcaa
     3061 ctcctggaag gcatatcaga caacaagacc attctagagt tcgacctgcg actgtcagat
     3121 gtatcccagg agagcgagta cctcattggc caggttctgc atgccaaccg agaggctgcc
     3181 cgccagcgga ccctgaatcc tggccacttc tcctcaccca ctaacaactg tactgagaat
     3241 tctgtagtcg atatcaagct tgtcgacgcg gccctcgagg ctagcggcgt tgccatgcca
     3301 ggtgccgaag atgatgtggt gtaaatcgag actcctcagg tgcaggctgc ctatcagaag
     3361 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3421 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3481 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3541 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3601 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     3661 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     3721 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     3781 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     3841 ctcttatgaa gatccctcga cttaattaag gtacccaatt cgccctatag tgagtcgtat
     3901 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3961 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4021 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4501 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4561 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5521 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5581 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5821 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6481 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6541 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6601 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6661 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6721 aaaagctg
//
