LOCUS       sequence-348289-        7791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse dynein regulatory complex subunit 7 
            (Drc7) tagged with FLAG at C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, 
            Shiba K, Inaba K, Ikawa M
  TITLE     Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is 
            required for sperm flagellum formation and male fertility in mice.
  JOURNAL   PLoS Genet. 2020 Jan 21;16(1):e1008585. doi: 
            10.1371/journal.pgen.1008585. eCollection 2020 Jan.
  PUBMED    31961863
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2020-01-21"; volume: "16(1):e1008585. doi"; pages: "
            10.1371/journal.pgen.1008585. eCollection 2020 Jan"
COMMENT     Sequence Label: pCAG-mDrc7-FLAG
FEATURES             Location/Qualifiers
     source          1..7791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          640..1654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1580..1602
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1662..1681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1715..1724
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2904..2921)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4361..4384
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4471..4490)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4536..4591
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4590..4609)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4946..4965)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4947..4965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4975..4992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4975..4991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4984..5006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5133..5588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5614..5718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5719..5787,5788..6579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5937..5956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6750..7338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7239..7258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7492..7509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7626..7647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7662..7667,7668..7685,7686..7692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7700..7716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7705..7727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7724..7740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7724..7740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7759..7779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7761..7779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg ggggggcggg gcggggcgag gcggagaggt
      541 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      601 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      661 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      721 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      781 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      841 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      901 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      961 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1021 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     1081 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     1141 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     1201 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1261 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     1321 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1381 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1441 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1501 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1561 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagcctctgc
     1621 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1681 gctgtctcat cattttggca aatctagagg atccgccgcc atggaggtcc tgagagagaa
     1741 agtggaggag gaggaggagg ctgagcgtga agaagcagcc gagagggccg aacggactga
     1801 gaagctggag agggtgacaa agtccgccga agtgagcaga gaggggacca tcctgagcca
     1861 ggatgagctt cgagacctgg aggggaagct gatggccatc gagatcccta cccaagctga
     1921 tcactcggtc ataagccagg cccctgttga tgtcaccaag ctgcctcctt cctataccac
     1981 caactcattg aaggaggagc acctgctgct ggtggcagac aacttctccc gccagtacag
     2041 ccacctgtgt ccagaccgcg tgcctctctt cttgcacccg ttaaatgagt gcaatgtgcc
     2101 taagttcgtg agcaccaccc tgaggcccac actgatgccc taccctgaac tctacaactg
     2161 ggacagctgc gcccagtttg tatctgactt cctcaccatg gtcccactgg ttgatcccct
     2221 caagccgccc acgcacttat attcctcgac caccgtgctc aagtgccaga agggaaactg
     2281 ctttgacttc agcaccctgc tctgctccat gttgattggc tccggctatg atgcctactg
     2341 cgtcaatggc tatggctcac tggacctgtg cctcatggac ctgacaaggg aagtgtgtcc
     2401 gctcactgtg aaggccaaag agatagtcaa gaagaaagag aagacggtgc ctaagaaata
     2461 ctccatcaag ccgccccggg acctgaccag caagtttgag caggagcaag aggagaagag
     2521 aatacaggag atcaaggatc tagaacaaag gcgacttaag gaagaggaag atcgcatcct
     2581 ggaggcagag aaagcgaagc ccgaccccct gcatggcctg cgggtacact cctgggtcct
     2641 ggtgctggca ggaaagcgag aggtgcctga gagcttcttc atcgaccctc tgactgcacg
     2701 cagctacagt accaaggatg agcacttcct gggcattgag agcctctgga accacaagaa
     2761 ctactggatc aacatgcagg attgctggaa ctgctgcaag gacttgatct tcgacctggg
     2821 agaccctgta agatgggagt acatgctctt ggggaccgat aagcctcacc tgtccttgac
     2881 tgaggaggat gaagaagggc tggatgatga tgatgatgat gtggaagacc tgggcaaaga
     2941 agaggaggat aagagctttg acatgccgtc ttcgtgggtc tcccagattg agatcacccc
     3001 agaagagttt gagacccgtt gtcccagtgg gaagaaagtg atacagtaca agaaggcaca
     3061 gctggagaag tggtccccat acctcaacaa caacggcctg gtgtgtcgcc tcaccaccta
     3121 cgaggaccaa cagtgcacaa aggttctaga gataaaggag tggtaccaga accgggagga
     3181 tatgctagag ctgaagcaca tcaacaagac cacaggcctg cacgtggact acttcaagcc
     3241 aggccatccc caagctctgt gtgtgcactc atacaagtct atgctacctg agatggaccg
     3301 cgtcatggag ttctataaaa aaatccgtgt ggatggcctg gtgaagcggg aggagacgcc
     3361 catgacaatg accgagtact atgaagggcg ctccgacttc cttgcctatc ggcacgtcaa
     3421 ttttggaccc agagtcaaga agctcagcca gagcagcgtg gagtcgaacc caaggcccat
     3481 ggtgaaaatc acagagcggt tctcccgcaa tccagagaag cccgcggatg aggacgtggc
     3541 agagcgtttg tttctgatag tggaagagcg catccagctt cgctaccatt gccgcgacga
     3601 ctacatcacc gcatccaagc gagagttcct gcggcgcatg gaggtcgaca gcaagggaaa
     3661 caagatcatc atgacaccag aaatgtgcat cagctacgag gtagagccga tggagcacac
     3721 taagaaactc ctctaccagt acgagaccat gaatcagctg aagaacgaag aaaagctgtc
     3781 ccgacatcag gcctgggagt cggagctgga ggtgctggag attttgaagc tccgtgaaga
     3841 ggaggaggag gcacacacgc tgaccatctc catctacgac accaagcgca acgagaagag
     3901 caaagagtat cgggaggcca tggagcgcgt cttgcatgag gagcacctgc ggcaggtgga
     3961 ggcccagctg gactacctgg ccccattcct ggcccagctc cctccaggcg agaagctaac
     4021 ccgttggcag gcggtgcgtc tcaaggacga gtgtctcagc gacttcaagc agcggctcat
     4081 cgacaaggct aacctcatcc aggctcgctt tgagaaggag acccaggagc tgcagaagaa
     4141 gcagcaatgg taccaggaga accaggtaac cctgacgccg gaggatgaaa acctgtacct
     4201 gagctactgt tctcaggcca tgttccgcat ccgaatcttg gaacagcggc tcaatcggca
     4261 caaagagctg gctccactga agtacctggc gctggaggaa aagctctaca aagacccacg
     4321 cttaatagac ttcgtaaaag tctttgttga attcgatatc gactacaagg atgacgatga
     4381 caagtagctc gagactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     4441 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     4501 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     4561 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     4621 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     4681 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     4741 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     4801 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     4861 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     4921 cgacttaatt aaggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4981 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5041 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5101 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5581 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5641 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5701 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5761 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5821 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5881 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5941 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6001 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6061 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6121 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6181 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6241 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6301 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6361 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6421 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6481 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6541 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6601 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6661 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7381 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7441 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7501 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7561 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7621 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7681 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7741 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct g
//
