LOCUS       sequence-348302-        9349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3160
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2103..2127)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2135..2176
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2287..2875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2340..2360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2758..2769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2947..3180
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3258..3379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3295..3314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3349..3368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3419..3554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3574..3593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3575..3593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3603..3620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3603..3619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3612..3634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3761..4216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3848..3867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4058..4079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4242..4346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4347..4415,4416..5207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4565..4584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5378..5966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5867..5886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6120..6137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6254..6275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6290..6295,6296..6313,6314..6320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6328..6344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6333..6355
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6352..6368
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6352..6368
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6387..6407
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6389..6407
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6435..6661
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6662..6842
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6889..7014
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7507..7740
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7925..7969
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8200..8410
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8222..8418
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8269..8404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8450..9052
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8450..8469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8946..8966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9095..9212
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgcc gcgctccttc ctggtgaaaa cgcactccag
     1261 ccacagggtc cccaactacc ggcggctgga gacgcagaga gaaatcaatg gtgcctgctc
     1321 tgcctgtggg gggctggtgg tgcccctcct cccccgagac aaggaggccc cttctgtgcc
     1381 cggtgacctt ccccagccct gggaccgctc ctcggccgtc gcctgcatct ccctgcccct
     1441 cctgccacgg atcgaggaag ctctgggggc ctctgggctg gacgccttgg aagtcagcga
     1501 ggtcgaccct cgggccagcc gggccgccat tgtacccctc aaagacagcc tgaaccacct
     1561 caacctgccc ccactgctgg tgctgcccac acggtggtcc ccgaccttgg gcccagaccg
     1621 gcacggggct ccggaaaaac tgcttggggc tgagcggatg ccccgagccc cgggcggctt
     1681 tgagtgcttc cactgccaca aaccctacca cacgctggcc gggctggcca ggcaccggca
     1741 gctgcactgc cacctgcagg tggggcgtgt cttcacctgc aagtactgcg acaaggagta
     1801 caccagcctg ggtgccctca agatgcacat ccgcactcac acgctgccct gcacctgcaa
     1861 gatctgtggc aaggccttct ccaggccctg gttactgcag ggccatgtcc gcacccacac
     1921 aggggagaag ccctatgcct gctcgcactg cagcagggcc tttgccgacc gctccaacct
     1981 tcgggcccat ctgcaaacgc actcagacgc caagaagtac cggtgccggc gctgcaccaa
     2041 gaccttctcc cgcatgtccc tcctggcgcg gcatgaggag tctggctgct gcccgggccc
     2101 cttgccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     2161 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     2221 gctttatata tcttgtggaa aggacgaact tggcagctgt tagttgcctt tacgcgttaa
     2281 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2761 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2821 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2881 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2941 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3181 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3241 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3361 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3541 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3601 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3661 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3721 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3901 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3961 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4021 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4081 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4141 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4201 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4321 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4381 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4441 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4501 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4561 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4621 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4681 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4741 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4801 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4861 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4921 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4981 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5041 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5101 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5161 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5221 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5281 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5341 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5401 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5461 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5521 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5881 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6001 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6061 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6241 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6301 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6361 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6421 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6481 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6541 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6601 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6661 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6721 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6781 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6841 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6901 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6961 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7021 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7081 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7141 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7201 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7261 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7321 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7381 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7441 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7501 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7561 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7621 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7681 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7741 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7801 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7861 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7921 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7981 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8041 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8101 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     8161 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8221 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8281 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8401 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     8461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8641 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     8701 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8881 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     9061 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9121 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9181 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9241 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9301 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
