LOCUS       sequence-348304-       10384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3161
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3138..3162)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3170..3211
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3322..3910
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3375..3395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3793..3804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3982..4215
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4293..4414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4330..4349)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4384..4403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4454..4589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4609..4628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4610..4628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4638..4655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4638..4654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4647..4669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4796..5251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4883..4902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5093..5114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5277..5381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5382..5450,5451..6242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5600..5619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6413..7001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6902..6921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7155..7172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7289..7310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7325..7330,7331..7348,7349..7355)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7363..7379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7368..7390
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7387..7403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7387..7403
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7422..7442
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7424..7442
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7470..7696
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7697..7877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7924..8049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8542..8775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8960..9004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9235..9445
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9257..9453
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9304..9439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9485..10087
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9485..9504)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9981..10001
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10130..10247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgcaaga aaccttcagg aacctggcct ctatagggaa
     1261 aaaatggaaa ccccagaaca tatatgtaga gtacgaaaat ctaaggagaa acctaagaat
     1321 tgtgggagag agactctttg aaagtaaaga aggtcatcag catggagaaa ttttgaccca
     1381 ggttccagat gacatgctga agaaaacaac tactggagta aaatcatgcg aaagcagtgt
     1441 gtatggagaa gtaggcagtg ctcattcatc tcttaatagg cacatcagag atgacactgg
     1501 acacaaggca tatgagtatc aagaatatgg acagaaacca tataaatgta aatactgtaa
     1561 aaaacctttc aactgtctct cctctgttca gacacatgaa agggctcata gtggaaggaa
     1621 actctatgtt tgtgaggaat gcggaaaaac atttatttcc cattcaaacc ttcaaagaca
     1681 caggataatg caccgtggag atggacctta taagtgtaaa ttttgtggga aagccttgat
     1741 gtttctcagt ttgtatctta tccacaaacg aactcacact ggagagaaac catatcaatg
     1801 taaacagtgt ggtaaagcct ttagtcattc tagtagcctt cgaatacatg aaagaactca
     1861 cactggggag aagccttata aatgtaatga atgtgggaaa gcattccata gttccacatg
     1921 ccttcatgct cataaaagaa ctcacactgg ggagaagcca tatgaatgta aacagtgtgg
     1981 gaaagccttc agctcttccc attcctttca aatacatgaa agaactcaca cgggggagaa
     2041 gccatatgaa tgtaaggaat gtggaaaagc attcaagtgt cccagttctg ttcgcagaca
     2101 tgaaagaacc cactctagga aaaaacccta tgaatgtaaa cattgtggga aagtattatc
     2161 ttatcttacc agctttcaaa accacttggg aatgcacact ggagagatat ctcataaatg
     2221 taagatatgt gggaaagcct tttattctcc cagttcactt caaacacatg aaaaaactca
     2281 cactggagag aaaccctata aatgcaacca atgtggtaaa gcctttaatt cttccagttc
     2341 cttccgatat catgaaagaa ctcacactgg agagaaacct tacgagtgta agcaatgtgg
     2401 gaaagccttc agatctgcct cactccttca aacacatggt aggactcaca cgggagagaa
     2461 accctatgca tgtaaggaat gtggaaaacc atttagtaat ttctctttct ttcaaataca
     2521 tgaaaggatg cacagagaag agaagccgta tgaatgtaag ggttatggga aaacattcag
     2581 tttgcccagt ttatttcata gacatgaaag gactcacact ggaggaaaaa cctatgaatg
     2641 caagcagtgt ggcagatcct tcaactgttc gagctccttt cgatatcatg gaaggactca
     2701 cactggagag aaaccctatg aatgcaagca atgtggaaaa gccttcagat ctgcctcaca
     2761 gcttcaaatt catggaagga ctcacactgg agagaaacct tatgaatgta agcagtgtgg
     2821 gaaagccttt ggatctgcct cacaccttca aatgcatgga aggactcaca ctggagagaa
     2881 accctatgaa tgtaagcagt gtgggaagtc ttttggatgt gcctcgcgac ttcaaatgca
     2941 tggaaggact cacactggag agaaaccgta taaatgtaag caatgtggga aagcttttgg
     3001 atgtccctca aaccttcgaa ggcatggaag gactcacact ggagagaaac cctataaatg
     3061 taaccaatgt ggtaaagtct ttagatgttc ttcacaactt caagtgcatg gaagggctca
     3121 ctgcatagac accccatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     3181 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     3241 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaagatgaga caggccttgc
     3301 catggaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3361 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3421 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3481 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3541 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3601 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3661 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3721 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3781 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3841 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3901 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3961 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4021 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4081 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4141 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4201 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4261 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4321 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4561 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4621 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4681 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4741 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4801 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4861 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4921 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4981 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5041 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5101 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5161 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5221 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5521 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5581 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5641 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5701 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5761 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5821 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5881 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5941 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6001 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6061 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6121 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6181 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6241 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7201 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7261 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7321 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7381 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7441 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7501 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7561 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7621 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7681 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7861 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7921 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7981 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8041 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8101 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8161 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8221 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8281 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8341 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8401 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8461 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8521 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8581 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8641 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8701 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8761 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8821 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8881 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8941 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9001 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9061 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9121 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9181 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9241 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9361 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9421 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9481 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9541 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9601 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9661 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9721 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9781 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9841 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9901 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9961 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    10021 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    10081 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    10141 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10201 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10261 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
    10321 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
    10381 aatt
//
