LOCUS       sequence-348314-        4431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High constitutive expression of Firefly Luciferase in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMOD_A5803
FEATURES             Location/Qualifiers
     source          1..4431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1352..3004
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1418..1436)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      3014..3262
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3339..3443
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgcctg gcagacatac tgtcccacaa
      901 atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa aggttaggtc
      961 ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg tgcagtcggt
     1021 ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc caccatgtga
     1081 aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa taagtaaggg
     1141 tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct ccctcattgt
     1201 taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa gtagcctaga
     1261 agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag ccaagacgac
     1321 gaaaacaggt aagagctaag cttcctgcag gatggaagac gccaaaaaca taaagaaagg
     1381 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     1441 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     1501 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1561 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     1621 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     1681 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     1741 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     1801 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     1861 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     1921 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     1981 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     2041 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     2101 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     2161 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     2221 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     2281 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     2341 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     2401 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     2461 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     2521 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     2581 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     2641 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     2701 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg ctggtgtcgc
     2761 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     2821 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     2881 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     2941 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     3001 gtgacgtcga cgatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt
     3061 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
     3121 tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat aatccattgt
     3181 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
     3241 atggaatatg attaaagata agccagagct ctggtgacgg acgtgggcag gtgtgatgaa
     3301 aagccttagg gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat
     3361 ttttctaaat acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc
     3421 aattatattg aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct
     3481 tttttgcggc cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3541 atgctgaaga tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca
     3601 aaatcttaga gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc
     3661 tgctatgtgg cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca
     3721 tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg
     3781 atggcatgac agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg
     3841 ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca
     3901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa
     4021 ctggcgaact acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca
     4081 aagttgcagg accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat
     4141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga
     4321 aataaatagt agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct
     4381 tagctaccgt aggtcctctt aggcggtccg attagcgaca tagtcatcaa t
//
