LOCUS       sequence-348315-        5343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High constitutive expression of Firefly Luciferase in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMOD_C'5802
FEATURES             Location/Qualifiers
     source          1..5343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      147..735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     636..655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     889..906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2285..3937
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2351..2369)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        4318..4422
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttaccagt gtcgcttagc taccgtaggt cctcttaggc ggtccgatta gcgacatagt
       61 catcaatgcc ttgacggcaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacta
      241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      481 tcacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      781 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      841 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      901 agcgaggaag cggaagagcg cccacctgca acgccggggc gcgccctatg tcgagctgca
      961 ggtcaacgga tcaggatatt cttgtttaag atgttgaact ctatggaggt ttgtatgaac
     1021 tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa agaatgtttt
     1081 gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat tggactgaac
     1141 acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga attaaataac
     1201 aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt caccaacttg
     1261 atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca ataacactaa
     1321 aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt tacttttcca
     1381 agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa aaaacaaaaa
     1441 ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca atgcagtggg
     1501 acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa ataaaacgat
     1561 aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga tcttatgacg
     1621 accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc
     1681 cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa
     1741 taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt
     1801 attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc
     1861 ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca
     1921 atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc
     1981 tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata
     2041 tgttctttgg tttagattct gttaatctta gatcgaacac gattttctgg gtttgatcgt
     2101 tagatatcat cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa
     2161 tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag
     2221 tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacaggcctg
     2281 caggatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
     2341 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     2401 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
     2461 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     2521 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     2581 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
     2641 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     2701 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
     2761 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     2821 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
     2881 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
     2941 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     3001 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     3061 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
     3121 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     3181 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     3241 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     3301 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     3361 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     3421 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     3481 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     3541 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
     3601 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     3661 ccaacacccc aacatcttcg acgctggtgt cgcaggtctt cccgacgatg acgccggtga
     3721 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     3781 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     3841 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     3901 cataaaggcc aagaagggcg gaaagatcgc cgtgtgactc gaggttcgag tattatggca
     3961 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     4021 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     4081 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     4141 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     4201 ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgtg ttagagctct
     4261 cccactagca ggtgatgaaa agccttaggg tcaggtggca cttttcgggg aaatgtgcga
     4321 tgaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct aatgagacaa
     4381 taaccctgat aaatgcttca attatattga aaaaggaaga gtatgagtat ccaacatttc
     4441 cgtgtcgccc ttattccctt ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa
     4501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cccgagtggg ttacattgaa
     4561 ctggatctca acagcggcaa aatcttagag agttttcgcc ccgaagaacg cttcccaatg
     4621 atgagcactt tcaaagttct gctatgtggc gcggtgttat cccgtattga tgctgggcaa
     4681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ttcaccagtc
     4741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgtagtgc tgccataacc
     4801 atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc gaaggagcta
     4861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4981 actctccgca aactattaac tggcgaacta cttactcttg cttcacgcca gcaactcatt
     5041 gactggatgg aggcggacaa agttgcagga ccacttctgc gctcggcact tccggctggc
     5101 tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgcggtat cattgcagca
     5161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5281 taactgtcag acgaagggaa ataaatagta gcccgcctga tgtgcgggct tttttttggt
     5341 ggt
//
