LOCUS       sequence-348317-        5343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High constitutive expression of Firefly Luciferase in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMOD_D5802
FEATURES             Location/Qualifiers
     source          1..5343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..133
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1201..1789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1690..1709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1943..1960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3339..4991
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3405..3423)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg
      121 aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga
      301 gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg
      361 cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact
      721 acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg
      781 accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggc tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt
     1021 agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt
     1081 aggtcctctt aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc
     1141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1261 agcggtggtt tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt
     1321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1501 ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac
     1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccacc
     1981 tgcaacgtcc cggcgcgccc tatgtcgagc tgcaggtcaa cggatcagga tattcttgtt
     2041 taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc
     2101 cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat
     2161 taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc
     2221 caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac
     2281 ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa
     2341 tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca
     2401 caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca
     2461 cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt
     2521 ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt
     2581 tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac
     2641 gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg
     2701 agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa
     2761 ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt
     2821 gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt
     2881 agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc
     2941 caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct
     3001 ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt
     3061 cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat
     3121 cttagatcga acacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag
     3181 ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct
     3241 tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt
     3301 cgattaatct gagtttttct gattaacagg cctgcaggat ggaagacgcc aaaaacataa
     3361 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     3421 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     3481 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     3541 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     3601 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3661 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     3721 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     3781 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3841 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     3901 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     3961 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     4021 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     4081 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     4141 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     4201 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     4261 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     4321 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     4381 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     4441 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     4501 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     4561 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     4621 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     4681 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgctg
     4741 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4801 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4861 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4921 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     4981 tcgccgtgtg actcgaggtt cgagtattat ggcattggga aaactgtttt tcttgtacca
     5041 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     5101 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     5161 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
     5221 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     5281 aggagtaaaa cacttgtagt tgtgttagag ctcagtgact agcaggtgat gaaaagcctt
     5341 agg
//
