LOCUS       sequence-348319-        3884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YCR075C. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YCR075C. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCFB8455
FEATURES             Location/Qualifiers
     source          1..3884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(45..63)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(46..63)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(81..97)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..97)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(94..116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    105..121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(129..135,136..153,154..159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    174..195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(312..329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(483..1071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(563..582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1242..2033,2034..2102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1865..1884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2103..2207)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2275..2293
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2331..2353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2453..2472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2666..2688
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2680..2697
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2681..2697
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2704..2723
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2704..2722
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2754..2796
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           3713..3838
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 cccccccccc ctgcaggcgg ccgcctcgag gctagcttga gtattctata gtgtcaccta
       61 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      121 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      181 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      241 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      301 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      361 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1081 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1141 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1201 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1261 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1321 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1381 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1441 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1501 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1561 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1621 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1681 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1741 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1801 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1861 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1921 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1981 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2041 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2101 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2221 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2281 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2341 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2401 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2461 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2521 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2581 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2641 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2701 ttgtaatacg actcactata gggcgaatta attcgagctc ggtacccagc ttgcttgttc
     2761 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tcaattcccg gggctgaggc
     2821 ttaatatggt gtcgttagac gatatactag gtatcttgta tgttacgtca tggtcgatat
     2881 cgatgtatcc accgataatc accaattggc gccataagtc agcgagcgcg atatcgatgg
     2941 attttgtcat gttaaatacg gcaggttact cttacctggt catatccata tttttgcaat
     3001 tgtactgctg gaaaatgacg ggtgatgagt ctgacttggg caggcccaag ttgacgcaat
     3061 ttgatttctg gtattgcctg catgggtgct tgatgaatgt tgtcttattg acccaggtgg
     3121 tagctggagc gagaatctgg cgatttccag gtaaaggtca ccgcaagatg aatccatggt
     3181 acctaaggat tttactcgca tcactggcca ttttttcact gctaaccgta caatttatgt
     3241 actccaacta ctggtacgat tggcataact caagaactct ggcgtattgc aacaatttgt
     3301 ttttactcaa aatatcgatg tcactaatca agtacatccc acaagtgacg cataactcga
     3361 caagaaaatc tatggattgt ttccccattc agggtgtgtt tctagatgtc actggcggta
     3421 tcgcctcgct gctccaattg atttggcagt tgtctaacga tcaaggtttc agtctggata
     3481 cgttcgtgac aaattttgga aaagtgggac tgtcaatggt aactttaata ttcaacttca
     3541 tctttatcat gcagtggttt gtatatcgat ctcgaggcca tgatctggcg tcagagtacc
     3601 cgctgtgaat taaacctcag cgaattcgtg agaaagcttg ggaataggcc tttcagatct
     3661 gcccgagacc atggatgaag tatctagaaa tagcttgatc tggttaccac taaaccagcc
     3721 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     3781 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaaraaagt ttcttcacat
     3841 tctaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaamcc cccc
//
