LOCUS       sequence-34832-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Murakoshi H, Wang H, Yasuda R
  TITLE     Local, persistent activation of Rho GTPases during plasticity of 
            single dendritic spines.
  JOURNAL   Nature. 2011 Apr 7;472(7341):100-4. doi: 10.1038/nature09823. Epub 
            2011 Mar 20.
  PUBMED    21423166
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09823"; journalName: "Nature"; date: "2011-04-7- 7"; 
            volume: "472"; issue: "7341"; pages: "100-4"
COMMENT     Sequence Label: mCherry-Rhotekin(8-89)-mCherry-C1
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..761
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(199..217)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     467..486
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     661..684
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggaggacaac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      121 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      181 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
      241 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      301 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      361 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      421 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      481 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      541 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gccgaggtca
      601 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      661 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
      721 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtcc
//
