LOCUS       sequence-348330-        3580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 0 plasmid, contains AtUbi1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMC-1-AtUbi10-6
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1378..1396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1464..1568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1790..2581
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2752..3340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3241..3260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3494..3511
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcagga ggtcgagctg caggtcaacg gatcaggata ttcttgttta agatgttgaa
       61 ctctatggag gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc
      121 gaacttattc aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat
      181 attattggtc attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc
      241 attgatatat gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta
      301 acgagacttg ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata
      361 caaaaatatc caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata
      421 cgataaagaa gttacttttc caagaaattc actgatttta taagcccact tgcattagat
      481 aaatggcaaa aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg
      541 aaatacgctt caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg
      601 tatatttaaa aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc
      661 tcaacggctg gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa
      721 acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa
      781 atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa
      841 cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg
      901 acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt
      961 cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct caattctctc
     1021 taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     1081 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaac
     1141 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     1201 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     1261 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     1321 gtttttctga ttaacaggaa tgtgagacca cgaagtggct cttcagtgga cgaaagggcc
     1381 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1561 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1621 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1681 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1741 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1801 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1861 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1921 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1981 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2041 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2101 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2161 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2221 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2281 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2341 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2401 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2461 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2521 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2881 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3541 gcctctcccc gcgcgttggc cgattcatta atcactctgt
//
