LOCUS       sequence-348332-        2503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 0 plasmid, contains HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMC-9-HSP-11
FEATURES             Location/Qualifiers
     source          1..2503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..261
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(301..319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        387..491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             713..1504
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1675..2263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2164..2183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2417..2434
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcagct tatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg
       61 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
      121 attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata atccattgtg
      181 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
      241 tggaatatga ttaaagataa gcgcttgaga ccacgaagtg gctcttcagt ggacgaaagg
      301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      481 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      541 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
      601 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
      661 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
      721 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      781 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      841 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      901 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      961 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1021 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1081 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1141 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1201 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1261 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1321 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1381 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1441 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     1501 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1561 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1801 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2461 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgt
//
