LOCUS       sequence-348333-       17232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes STM and WUS2 for ectopic meristem induction in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 17232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pRN119
FEATURES             Location/Qualifiers
     source          1..17232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4575..6227
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4641..4659)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6237..6485
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        6631..6810
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6663..6846
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        9096..9441
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    10606..10780
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12462..12486
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13787..14416
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14845..15918
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15984..16178
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16414..16436)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16522..16662
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     16577..16596
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16677..16694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(16848..17232,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16928..16947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcaaagtt taacgcgtta
     3721 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
     3781 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
     3841 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
     3901 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
     3961 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
     4021 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
     4081 ctcggtaccg atcggcgcgc ctggcagaca tactgtccca caaatgaaga tggaatctgt
     4141 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
     4201 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
     4261 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
     4321 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
     4381 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
     4441 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
     4501 agtattcagg cacgtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
     4561 aagcttcctg caggatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     4621 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     4681 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     4741 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     4801 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     4861 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     4921 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     4981 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     5041 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     5101 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     5161 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     5221 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     5281 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     5341 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     5401 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     5461 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     5521 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     5581 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     5641 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     5701 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     5761 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     5821 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     5881 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     5941 ccatcttgct ccaacacccc aacatcttcg acgctggtgt cgcaggtctt cccgacgatg
     6001 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     6061 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     6121 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     6181 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtgacgt cgacgatatg
     6241 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     6301 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     6361 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     6421 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     6481 ataagccaga gctctggtga cggacggcgc gactagtttt acgtacgtta attaacccgg
     6541 gcgcgccgat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     6601 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     6661 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     6721 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     6781 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     6841 tctgcaccgt acctgcaggg tccgagctag gtcacagaag cgctcaggaa ggccgctgag
     6901 atagaggcat ggcggccaat gcgggcggcg gtggagcggg aggaggcagc ggcagcggca
     6961 gcgtggctgc gccggcggtg tgccgcccca gcggctcgcg gtggacgccg acgccggagc
     7021 agatcaggat gctgaaggag ctgtactacg gctgcggcat ccggtcgccc agctcggagc
     7081 agatccagcg catcaccgcc atgctgcggc agcacggcaa gatcgagggc aagaacgtct
     7141 tctactggtt ccagaaccac aaggcccgcg agcgccagaa gcgccgcctc accagcctcg
     7201 acgtgaacgt gcccgccgcc ggcgcggccg acgccaccac cagccaactc ggcgtcctct
     7261 cgctgtcgtc gccgccgcct tcaggcgcgg cgcctccctc gcccaccctc ggcttctacg
     7321 ccgccggcaa tggcggcgga tcggctgtgc tgctggacac gagttccgac tggggcagca
     7381 gcggcgctgc gatggccacc gagacatgct tcctccagga ctacatgggc gtgacggaca
     7441 cgggcagctc gtcgcagtgg ccacgcttct cgtcgtcgga cacgataatg gcggcggccg
     7501 cggcgcgggc ggcgacgacg cgggcgcccg agactctccc tctcttcccg acctgcggcg
     7561 acgacggcgg cagcggtagc agcagctact tgccgttctg gggtgccgcg tccacaactg
     7621 ccggcgccac ttcttccgtt gcgatccagc agcaacacca gctgcaggag cagtacagct
     7681 tttacagcaa cagcaacagc acccagctgg ccggcaccgg caaccaagac gtatcggcaa
     7741 cagcagcagc agccgccgcc ctggagctga gcctcagctc atggtgctcc ccttaccctg
     7801 ctgcagggag tatgtgagag caacgcgagc tgccactgct cttcacttat gtctctggaa
     7861 tggaaggagg aggaagtgag catagcgttg gtgcgttgct gtcattgtcc taggttagta
     7921 gctagtgcca gttactagta agcatcaggc ataggagtat gtagtagaag catgcacgtt
     7981 gccggccagc caggctttag acgggaaaag aatttggtgc agccggctgc aaaacaggat
     8041 gtttacagcc cccccctcga gccctagact tgtccatctt ctggattggc caagttaatt
     8101 aatgtatgaa ataaaaggat gcacacatag tgacatgcta atcactataa tgtgggcatc
     8161 aaagttgtgt gttatgtgta attactaatt atctgaataa gagaaagaga tcatccatat
     8221 ttcttatcct aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttt
     8281 attaaccaat tccatataca tataaatatt aatcatatat aattaatatc aattgggtta
     8341 gcaaaacaaa tctagtctag gtgtgttttg ctaattattg ggggatagtg caaaaagaaa
     8401 tctacgttct caataattca gatagaaaac ttaataaagt gagataattt acatagattg
     8461 cttttatcct ttgatatatg tgaaaccatg catgatataa ggaaaataga tagagaaata
     8521 attttttaca tcgttgaata tgtaaacaat ttaattcaag aagctaggaa tataaatatt
     8581 gaggagttta tgattagagc tctcccgcag atttgccttt tcaatttcag aaagaatgct
     8641 aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat ctacccgagc
     8701 aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     8761 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
     8821 ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc
     8881 tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac
     8941 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
     9001 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
     9061 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     9121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     9181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     9241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     9301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     9361 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     9421 tcatttcatt tggagagaac acgggggact atggagagtg gttccaacag cacttcttgt
     9481 ccaatggctt ttgccgggga taatagtgat ggtccgatgt gtcctatgat gatgatgatg
     9541 ccgcccatca tgacatcaca tcaacatcat ggtcatgatc atcaacatca acaacaagaa
     9601 catgatggtt atgcatatca gtcacaccac caacaaagta gttccctttt tcttcaatca
     9661 ctagctcctc cccaaggaac taagaacaaa gttgcttctt cttcttctcc ttcctcttgt
     9721 gctcctgcct attctctaat ggagatccat cataacgaaa tcgttgcagg aggaatcaac
     9781 ccttgctcct cttcctcttc ttcagcctct gtcaaggcca agatcatggc tcatcctcac
     9841 taccaccgcc tcttggccgc ttatgtcaat tgtcagaagg ttggagcacc accggaggtt
     9901 gtggcgaggc tagaggaggc atgctcgtct gccgcagccg ctgccgcatc tatgggacca
     9961 acaggatgtc taggtgaaga tccagggctt gatcaattca tggaagctta ctgtgaaatg
    10021 ctcgttaagt atgagcaaga gctctccaaa cctttcaagg aagctatggt cttccttcaa
    10081 cgtgtcgagt gtcaattcaa atccctctct ctatcctcac cttcctcttt ctccggttat
    10141 ggagagacag caattgatag gaacaataat gggtcatccg aggaagaagt cgatatgaac
    10201 aatgaatttg tagatccaca agctgaggat agagagctta aaggacagct cttgcgcaag
    10261 tacagtggtt acttagggag cctcaagcaa gagttcatga agaagaggaa gaaaggaaag
    10321 ctccctaaag aagctcgtca acaactgctt gattggtgga gccgtcacta caaatggcct
    10381 tacccttcgg agcaacaaaa gctcgccctt gcggaatcaa cggggctgga ccagaaacag
    10441 ataaacaatt ggttcataaa ccagaggaaa cggcattgga agccgtcgga ggacatgcag
    10501 tttgtagtaa tggacgcaac acatcctcac cattacttca tggataatgt cttgggcaat
    10561 cctttcccaa tggatcacat ctcctccacc atgctttgac tcgagtttct ccataataat
    10621 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
    10681 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
    10741 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta agtgtttgat
    10801 cgccggcggt accgagtgta cttcaagtca gtgggaaatc aataaaatga ttattttatg
    10861 aatatatttc attgtgcaag tagatagaaa ttacatatgt tacataacac acgaaataaa
    10921 caaaaaaaga caatccaaaa acaaacaccc caaaaaaaat aatcacttta gataaactcg
    10981 tatgaggaga ggcacgttca gtgactcgac gattcccgag caaaaaaagt ctccccgtca
    11041 cacatgtagt gggtgacgca attatcttta aagtaatcct tctgttgact tgtcattgat
    11101 aacatccagt cttcgtcagg attgcaaaga attatagaag ggatcccacc ttttattttc
    11161 ttcttttttc catatttagg gttgacagtg aaatcagact ggcaacctat taattgcttc
    11221 cacaatggga cgaacttgaa ggggatgtcg tcgatgatat tataggtggc gtgttcatcg
    11281 tagttggtga aatcgatggt accgttccaa tagttgtgtc gtccgagact tctagcccag
    11341 gtggtctttc cggtacgagt tggtccgcag atgtagaggc tggggtgtcg gattccattc
    11401 cttccattgt ccttgttaaa tcggccatcc attcaaggtc agattgagct tgttggtatg
    11461 agacaggatg tatgtaagta taagcgtcta tgcttacatg gtatagatgg gtttccctcc
    11521 aggagtgtag atcttcgtgg cagcgaagat ctgattctgt gaagggcgac acatacggtt
    11581 caggttgtgg agggaataat ttgttggctg aatattccag ccattgaagc tttgttgccc
    11641 attcatgagg gaattcttcc ttgatcatgt caagatattc ctccttagac gttgcagtct
    11701 ggataatagt tctccatcgt gcgtcagatt tgcgaggaga aaccttatga tctcggaaat
    11761 ctcctctggt tttaatatct ccgtcctttg atatgtaatc aaggacttgt ttagagtttc
    11821 tagctggctg gatattaggg tgatttcctt caaaatcgaa aaaagaagga tccctaatac
    11881 aaggtttttt atcaagctgg agaagagcat gatagtgggt agtgccatct tgatgaagct
    11941 cagaagcaac accaaggaag aaaataagaa aaggtgtgag tttctcccag agaaactgga
    12001 ataaatcatc tctttgagat gagcacttgg gataggtaag gaaaacatat ttagattgga
    12061 gtctgaagtt cttactagca gaaggcatgt tgttgtgact ccgaggggtt gcctcaaact
    12121 ctatcttata accggcgtgg aggcatggag gcaggggtat tttggtcatt ttaatagata
    12181 gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt gcgacaaggg ggggcccacg
    12241 ccgaatttaa tattaccggc gtggcccccc cttatcgcga gtgctttagc acgagcggtc
    12301 cagatttaaa gtagaaaatt tcccgcccac tagggttaaa ggtgttcaca ctataaaagc
    12361 atatacgatg tgatggtatt tgatggagcg tatattgtat caggtatttc cgttggatac
    12421 gaattattcg tacgaccctc atagtttaaa ctatcagtgt ttgacaggat atattggcgg
    12481 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
    12541 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
    12601 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
    12661 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
    12721 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
    12781 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
    12841 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
    12901 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
    12961 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
    13021 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
    13081 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
    13141 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
    13201 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
    13261 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
    13321 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
    13381 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
    13441 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
    13501 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
    13561 ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg
    13621 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
    13681 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
    13741 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
    13801 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
    13861 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
    13921 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
    13981 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
    14041 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
    14101 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
    14161 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
    14221 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
    14281 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
    14341 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
    14401 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
    14461 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
    14521 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
    14581 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
    14641 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
    14701 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
    14761 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
    14821 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
    14881 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
    14941 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
    15001 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
    15061 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
    15121 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
    15181 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
    15241 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
    15301 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
    15361 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
    15421 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
    15481 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
    15541 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
    15601 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
    15661 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
    15721 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
    15781 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
    15841 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
    15901 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
    15961 aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
    16021 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
    16081 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
    16141 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
    16201 caagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
    16261 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
    16321 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
    16381 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac
    16441 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    16501 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    16561 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    16621 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggccctcttc cgcttcctcg
    16681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    16741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    16801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    16861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    16921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    16981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    17041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    17101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    17161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    17221 agagcgaggt at
//
