LOCUS       sequence-348361-        3969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus oocyte expression vector containing YCL002C. Contains T7 RNA
            polymerase binding site, and adds polyA tail to YCL002C. Used for 
            generation of mRNA, for injection into xenopus laevis oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Moller-Hansen I, Saez-Saez J, van der Hoek SA, Dyekjaer JD, 
            Christensen HB, Wright Muelas M, O'Hagan S, Kell DB, Borodina I
  TITLE     Deorphanizing solute carriers in Saccharomyces cerevisiae for 
            secondary uptake of xenobiotic compounds.
  JOURNAL   Front Microbiol. 2024 Apr 12;15:1376653. doi: 
            10.3389/fmicb.2024.1376653.
  PUBMED    38680917
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2024-04-12"; volume: "15:1376653. doi"; pages: "
            10.3389/fmicb.2024.1376653"
COMMENT     Sequence Label: pCfB8155
FEATURES             Location/Qualifiers
     source          1..3969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(584..1375,1376..1444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1207..1226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1445..1549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1617..1635
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1673..1695)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1795..1814
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2096..2138
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           3140..3265
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(3356..3374)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3357..3374)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3392..3408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3392..3408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3405..3427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3416..3432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3440..3446,3447..3464,3465..3470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3485..3506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3623..3640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3794..3969,1..413))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3874..3893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
       61 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      241 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1561 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1621 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1681 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1741 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1801 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1861 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat taattcgagc tcggtaccca gcttgcttgt
     2101 tctttttgca gaagctcaga ataaacgctc aactttggca gatcaattcc cggggctgag
     2161 gcttaatatg cttgttattg ttctgcaggg cttggcaggt tttttatcag tatgcataat
     2221 tgaaggagaa agtatacatg aaaggtttga ctaactaagg gagaaaactt ccatttctta
     2281 catagatcat cgccattcta tgtcaaaagc gctataataa acttcatcga tctatttacg
     2341 ggctttcata tgatttattt ctgctagatt ttgttggtaa tggtctgtac ttgtattgcg
     2401 cgttgcatta ttgttattca tctctggtga gggaacaact gtctcaaaga ttcccacttt
     2461 tttatccgtt gaatgatgcc cgtagtatac ctatatccag tttcctcatt ctaaaagatt
     2521 tttgcgtttc ctgttgctgc atgatggttt taaggcagtt atactattat aggtcaacta
     2581 aacatattta tcagggcatt tccattactt ccataataat tataagtgtg ttccttgtat
     2641 taggcatatt tacatatggc tgttccatct ctaatttacc gttgaaaaat tcaggtaaat
     2701 ttggcgtttt ctatttggaa cacataaatt atttgtgggt aatggcaaat ctgcttaaat
     2761 gtttcaagta tgtgcctcaa atgagtataa actggatggg atgctcaaca gtaggtttat
     2821 catcaaagtt tgcattgata tcatttttgg ctgaatctat cgatttatta ggtagactgg
     2881 taataccgac caatgcattg ttttacgaga taccatttaa ttcaacgccc ttttgggtaa
     2941 aattgataca atttgtcacc ttattggtca tactttgtca agttcagtat gtttacgtag
     3001 gacggaaacc acggttgcca aaaggaaaac tatgaattaa acctcagcga attcgtgaga
     3061 aagcttggga ataggccttt cagatctgcc cgagaccatg gatgaagtat ctagaaatag
     3121 cttgatctgg ttaccactaa accagcctca agaacacccg aatggagtct ctaagctaca
     3181 taataccaac ttacacttta caaaatgttg tcccccaaaa tgtagccatt cgtatctgct
     3241 cctaataaaa araaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3301 aaaamccccc cccccccccc cctgcaggcg gccgcctcga ggctagcttg agtattctat
     3361 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3421 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3481 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3541 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3601 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3661 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3721 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3841 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3901 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3961 tcccttcgg
//
