LOCUS       sequence-348368-        9281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon to generate a Tet inducible SOX17 expressing 
            cell line. Tet-On inducible when paired with #169175.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Jung HS, Uenishi G, Park MA, Liu P, Suknuntha K, Raymond M, Choi YJ,
            Thomson JA, Ong IM, Slukvin II
  TITLE     SOX17 integrates HOXA and arterial programs in hemogenic endothelium
            to drive definitive lympho-myeloid hematopoiesis.
  JOURNAL   Cell Rep. 2021 Feb 16;34(7):108758. doi: 
            10.1016/j.celrep.2021.108758.
  PUBMED    33596423
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-02-16"; volume: "34(7):108758. doi"; pages: " 
            10.1016/j.celrep.2021.108758"
COMMENT     Sequence Label: pTRE-SOX17-P2A-Venus-rpEF1a-Zeo
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1163
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1154
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1111..1131
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1196..1570
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1574..1695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1585..1604)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1639..1658
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    1838..2045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2034..2051
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2067..2780,2781..2783,2784..2786))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2112..2133)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2461..2480
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2720..2741
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2787..2843)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3129..3146)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             complement(3135..3152)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     promoter        complement(4114..4428)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(4117..4141)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4188..4206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4224..4242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4259..4277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4295..4313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4331..4349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4366..4384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4402..4420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4836..4854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4861..4880
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4861..4879
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4904..4920
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4905..4921
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5204..5238
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5729..5745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5729..5745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5742..5764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5753..5769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5777..5783,5784..5801,5802..5807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5822..5843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5960..5977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6131..6719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6211..6230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6890..7681,7682..7750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7513..7532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7751..7855)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7881..8336)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8018..8039)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8230..8249
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8463..8485
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8477..8494
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8478..8494
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8624..8686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8882..8898)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8882..8898)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9227..9247)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg ggcgggcctt
      361 gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
      421 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
      481 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
      541 atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
      661 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
      721 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
      781 gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg cgctcgggag
      841 agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca gccgtcgctt
      901 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tggagctttt
      961 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     1021 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     1081 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     1141 ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca tctagagcaa ctagcatggc
     1201 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     1261 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     1321 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     1381 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     1441 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     1501 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     1561 gcaggactga atctaagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1621 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1681 tgcaataaac aagttaacaa caacaattgc attcatttta tgcttcaggt tcagggggag
     1741 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     1801 ccggctgcct cgcgcatgca tcatagagcc caccgcatcc ccagcatgcc tgctattgtc
     1861 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
     1921 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
     1981 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
     2041 cacagtcgag gctgatcagc ccatgattac ttgtacagct cgtccatgcc gagagtgatc
     2101 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     2161 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     2221 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg
     2281 cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
     2341 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
     2401 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
     2461 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
     2521 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc
     2581 aggccgtagc ccagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
     2641 cagatcagct tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
     2701 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
     2761 aacagctcct cgcccttgct caccatgggt ccagggttct cctccacgtc tccagcctgc
     2821 ttcagcaggc tgaagttagt agctccgctt ccgtatacca cgtcaggata gttgcagtaa
     2881 tataccgcgg agctggcgtc ggacaccacc gaggaaatgg ccccgtggct gtcggggaga
     2941 ttcacaccgg agtcatgccc ctggtagggg aggcccatct caggcttgca cacgaagtgc
     3001 agatactgtt caaattccgt gcggtccacc tccccgagga gctcggcggg ctgactgggg
     3061 tccgtgccgt cccggcaggg cagtgcctcc ggagggggcg acggctgtcc ggggcccggg
     3121 gggtggtgct ggtgctggtg ctggtgctgg tgttgcggct gcatctggaa gccgcgcccg
     3181 ccgcccgccc cgggcgagcc catcgcgccg tagtacacgt gaagggcgct gggtggcgcc
     3241 aggaggcccg gaatcgaggg acccgcgggc tctgggccga gtcgggggtg catgggaccg
     3301 gcgggaggct ccggggggcc agcgtagtcc gagacctgcg cgtagctgta ggtgccggcc
     3361 gccgggcagt ccccgggcat cggggcggcg aagaaagccg ggtcggggtc cacgccgtcc
     3421 agcggggacg tgtcgggcgt gggcaacggg tagccgtcga gcggaggcgc gcccagactc
     3481 tggcagtcgc ggtagtggcc gcccatgtgc ggaggcagca gcggcgggcc ggcggggaag
     3541 ccctgctcgg ggaactggag gcccaggccg tccatggcca cgcggccgcc ctcggggccc
     3601 agcgcggccg cctgcggctc agccaggccg tgcaggaagc cgccctccac ccgcttcagc
     3661 cgcttcacct gcttgcgccg ccgcggccgg tacttgtagt tggggtggtc ctgcatgtgc
     3721 tgcacgcgca gccgctctgc ctcctccacg aagggccgct tctccgccag cgtcagcgcc
     3781 ttccacgact tgcccagcat cttgctcaac tcggcgttgt gcaggtctgg attctgctgc
     3841 gccagccgct tgcgctcgtc cttagcccac accatgaaag cgttcatcgg ccgccggata
     3901 cgggactcgc ccttggctcg gcccgcggcc ccggccggtg ctccgctgtt cgccggcgcc
     3961 tcgcccttca ccttcatgtc cccgatgggg ctcagcgact cggcccaggg gcaggggccc
     4021 agcccggcca tcaccgcggg cagcgcgctc tgggtctggc tctggtcgtc actggcgtat
     4081 cccgcatccg ggctgctcat gctagctcct ccaggcgatc tgacggttca ctaaacgagc
     4141 tctgcttata taggcctccc accgtacacg cctacctcga catacgttct ctatcactga
     4201 tagggagtaa actcgacata cgttctctat cactgatagg gataaactcg acatacgttc
     4261 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggagtaaact
     4321 cgacatacgt tctctatcac tgatagggag taaactcgac atcgttctct atcactgata
     4381 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcgc tcgagacgcg
     4441 tatacctagg atccggccgg cctgcaggtg tcctcacagg aacgaagtcc ctaaagaaac
     4501 agtggcagcc aggtttagcc ccggaattga ctggattcct ttttagggcc cattggtatg
     4561 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     4621 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     4681 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     4741 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     4801 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattagatct
     4861 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
     4921 gataagcttg atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa
     4981 catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat
     5041 catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt
     5101 gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac
     5161 ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg
     5221 cagactatct ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc
     5281 cggctcagtc atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc
     5341 ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt
     5401 gacgttgagc gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc
     5461 tttaacggtg aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg
     5521 acacagttcc ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc
     5581 cggaactgcc gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc
     5641 agcgaggacg ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta
     5701 cccggcgggc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5761 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5821 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5881 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5941 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6001 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6061 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6541 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6661 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7021 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7081 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7141 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7201 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7261 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7321 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7381 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7441 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7501 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7561 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7621 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7681 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7741 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7801 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7861 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7921 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7981 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8041 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8101 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8161 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8221 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8281 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     8341 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8401 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8461 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt
     8521 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
     8581 gatgaagatg ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca
     8641 tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct
     8701 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
     8761 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac
     8821 aaaaactcaa aatttcttct ataaagtaac aaaactttta tcgaattcct gcagcccggg
     8881 ggatccacta gttctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     8941 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     9001 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     9061 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     9121 ggggaaaagg cctccaaggc cagcttccca caataagttg ggtgaatttt ggctcattcc
     9181 tcctttctat aggattgagg tcagagcttt gtgatgggaa ttctgtggaa tgtgtgtcag
     9241 ttagggtgtg gaaagtcccg cgatcgatcc tagcttcgtg a
//
