LOCUS       sequence-348373-        7050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SOUL in neuronal cell types.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Gong X, Mendoza-Halliday D, Ting JT, Kaiser T, Sun X, Bastos AM, 
            Wimmer RD, Guo B, Chen Q, Zhou Y, Pruner M, Wu CW, Park D, 
            Deisseroth K, Barak B, Boyden ES, Miller EK, Halassa MM, Fu Z, Bi G,
            Desimone R, Feng G
  TITLE     An Ultra-Sensitive Step-Function Opsin for Minimally Invasive 
            Optogenetic Stimulation in Mice and Macaques.
  JOURNAL   Neuron. 2020 Jul 8;107(1):197. doi: 10.1016/j.neuron.2020.06.018.
  PUBMED    32645306
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2020-07-8"; volume: "107(1):197. doi"; pages: " 
            10.1016/j.neuron.2020.06.018"
COMMENT     Sequence Label: pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA
FEATURES             Location/Qualifiers
     source          1..7050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   158..298
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   158..287
                     /label=AAV2 ITR
     rep_origin      373..828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(460..479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     670..691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(845..864)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     964..986
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1024..1042)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1110..1214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1215..1283,1284..2075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1433..1452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2246..2834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2735..2754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2896..3025
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        3078..3525
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3573..3582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3579..4505
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTSPVILIHLSNLTGLSNDYSRRTMGLLVSAIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             4524..4580
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4581..6011
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGIGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    6072..6660
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6125..6145)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6543..6554)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    join(6692..7050,1..118)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6891..6910)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
       61 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
      121 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
      181 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
      241 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
      301 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
      361 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      421 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
      481 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      541 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
      601 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      661 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
      721 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
      781 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
      841 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      901 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      961 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     1021 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1081 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1141 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1201 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1321 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1381 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1441 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1501 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2701 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2821 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2881 cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg
     2941 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     3001 gccaactcca tcactagggg ttcctgcggc cgcacgcgtt taattaagtg tctagactgc
     3061 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
     3121 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
     3181 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
     3241 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
     3301 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
     3361 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
     3421 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
     3481 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaaccggcta
     3541 gaggatcctt cgaaaccggt gctagcgcgg ccgccaccat ggactatggc ggcgctttgt
     3601 ctgccgtcgg acgcgaactt ttgttcgtta ctaatcctgt ggtggtgaac gggtccgtcc
     3661 tggtccctga ggatcaatgt tactgtgccg gatggattga atctcgcggc acgaacggcg
     3721 ctcagaccgc gtcaaatgtc ctgcagtggc ttgcagcagg attcagcatt ttgctgctga
     3781 tgttctatgc ctaccaaacc tggaaatcta catgcggctg ggaggagatc tatgtgtgcg
     3841 ccattgaaat ggttaaggtg attctcgagt tcttttttga gtttaagaat ccctctatgc
     3901 tctaccttgc cacaggacac cgggtgcagt ggctgcgcta tgcagagtgg ctgctcactt
     3961 ctcctgtcat ccttatccac ctgagcaacc tcaccggcct gagcaacgac tacagcagga
     4021 gaaccatggg actccttgtc tcagccatcg ggtgtatcgt gtggggggct accagcgcca
     4081 tggcaaccgg ctatgttaaa gtcatcttct tttgtcttgg attgtgctat ggcgcgaaca
     4141 cattttttca cgccgccaaa gcatatatcg agggttatca tactgtgcca aagggtcggt
     4201 gccgccaggt cgtgaccggc atggcatggc tgtttttcgt gagctggggt atgttcccaa
     4261 ttctcttcat tttggggccc gaaggttttg gcgtcctgag cgtctatggc tccaccgtag
     4321 gtcacacgat tattgatctg atgagtaaaa attgttgggg gttgttggga cactacctgc
     4381 gcgtcctgat ccacgagcac atattgattc acggagatat ccgcaaaacc accaaactga
     4441 acatcggcgg aacggagatc gaggtcgaga ctctcgtcga agacgaagcc gaggccggag
     4501 ccgtgccagt taacggaagc ggagccacta acttctccct gttgaaacaa gcaggggatg
     4561 tcgaagagaa tcccgggcca atggtgagca agggcgagga ggtcatcaaa gagttcatgc
     4621 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     4681 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     4741 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     4801 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     4861 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     4921 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     4981 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     5041 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     5101 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     5161 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     5221 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcatc ggcagcaccg
     5281 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt
     5341 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     5401 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     5461 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     5521 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     5581 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     5641 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     5701 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     5761 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     5821 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     5881 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     5941 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
     6001 tgtacaagta aggcgcgaat cgcgagtcga cggcgcgccc ctgcagggaa ttcgatatca
     6061 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6121 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6181 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6241 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6301 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6361 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6421 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6481 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6541 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6601 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6661 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     6721 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     6781 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     6841 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     6901 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     6961 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     7021 cagctaattt ttgttttttt ggtagagacg
//
