LOCUS       sequence-348385-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0065
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(12..55)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2129..2186
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2210..2227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2381..2969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2461..2480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3152..3246)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3267..3926)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3841..3860
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     join(4088..4098,1..8)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
       61 ttcggtctca aatggctgct gctcatgggc atgctcataa tgttgatgaa aattaccaag
      121 tcctagtgaa gaagcagcct ttgaattttt caggagataa gccttttact cctattttgg
      181 ataccatcaa ctaccctacc catatgaaga atctctctct tcaggagctt caacaattag
      241 ctgacgaatt aagggaggag atagtgtatt ctgtggccaa aacgggtggt cacttaagtt
      301 ccagcctagg tgtggccgag ctcactgtgg cactccacca cgtgttcgaa accccaaatg
      361 ataaaatcat atgggatgtt ggccaccaga catatgggca taagattctg acagggagaa
      421 ggtcaaagat gcataccatg agacaaactt gcggacttgc aggtttcccc aaaagagatg
      481 aaagtgccca tgatgcattt ggagttggcc atagttccac cagcatttct gccggtttag
      541 gaatggcaat aggacgagat ttattaggta agaaaaatca tgtaataacg gtaataggag
      601 atggagctat gacagccgga caagcatatg aagccatgaa taatgctgga tatcttgatt
      661 ctaatctcat aatcatattg aatgataaca aacaagtttc tctcccgact gccacaattg
      721 atggtcctgc acctcctact ggtgctctaa gcaatgcctt aactaggctt caatctagta
      781 gaaaatttcg actacttcgg gaggctgcaa aaggtgtgac aaagcaatta gggggacaag
      841 ttcatgaaat ggcagctaaa gtggatacat atatgagagg gatgaatagc caaagggctt
      901 ccttatttga agatttagga atgtattata ttggtccact tgatggtcac aatatggaag
      961 atcttgttga tattttagag aaagtaaaat ctgttcctgc aacgggccct gttcttattc
     1021 atattattac tgagaaagga aaaggatatc ctcctgctga agctgccgcc gacaaaatgc
     1081 atggggtggt gaaatttgag ccaatgacag gaaaacaatt caagtcaaag tcaaaaacaa
     1141 agtcatatac aaattatttt gctgagagtt taataagtga agctagggaa gatgataaga
     1201 ttattgccat tcatgctgct atgggaggag gaacaggact caatttattc cagaaacact
     1261 ttcctgaacg atgctttgat gttgggatag ctgagcaaca tgctgtcact tttgctgctg
     1321 gcttagcttc tgaaggactc aagccttttt gtgctattta ctcatctttc ttacaaagag
     1381 gttatgatca ggtggttcat gatgtggacc ttcaaaaaat tccagtaagg tttgctatgg
     1441 atagagcagg gctggtgggt gcagatggac ccactcattg cggtgcattt gacacaactt
     1501 ttatggcttg tttgcctaat atggtggtca tggctccttc agatgaaacc gaattgatgc
     1561 acatggttgc cacagcagct gccattgatg ataggcctag ttgctttcga tatcccagag
     1621 gaaatggcat tggtgcaatt cttccaccaa acaacaaagg aactccatta cagattggta
     1681 aaggaagaat attgttggag ggaacaagag ttgccattct tgggtatgga gcaatggtgc
     1741 aaaactgttt aatggctgct cagcttcttg aaattaatgg aatatcagct acagttgttg
     1801 atgcccgatt ttgcaaacct cttgatggag atttgattag aaaattggct caacaacatg
     1861 aagtccttat cactgttgaa gaaggatcca ttggtggttt tggttcccat gtggcacagt
     1921 tcttggcttt gaatggattg cttgatggaa atttaaagtg gagggcaatg atgcttcctg
     1981 acagatatat tgatcatgga gctcaaaatg accaaattga agaggctggc ctaactccaa
     2041 agcatattgc tggcactgtg ttttctctac tcagcaaaaa caagaatact cttcaagttc
     2101 tccagtttta ggcttcgaga ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     2161 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     2221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
     2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3121 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     3181 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3241 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     3301 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     3361 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     3421 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3481 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     3541 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     3601 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     3661 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     3721 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     3781 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     3841 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     3901 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     3961 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4021 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4081 ctataaaaat aggcgtat
//
