LOCUS       sequence-348386-        3012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0818
FEATURES             Location/Qualifiers
     source          1..3012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     217..235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(239..282)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1270..1327
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1351..1368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1522..2110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1602..1621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2293..2387)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2408..3012,1..55))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2982..3001
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
       61 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      121 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
      181 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
      241 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcaaat
      301 gtgctcaaac acaaatgccc agttggccaa tttgtctgaa caagtaaagg ggaaggtcac
      361 tgaatttgat ttcaaggagt acatgcgttc caaggcaatg tcagtgaatg aggcactgga
      421 cagggctgtc ccacttcgtt atcctgaaaa aatacatgaa gctatgaggt attctcttct
      481 agcaggaggg aagcgagttc gtcctattct gtgcattgca gcatgtgagc ttgtgggagg
      541 gagcgaggag ctcgccatgc caactgcctg tgcaatggaa attatccaca caatgtcttt
      601 gattcatgat gacttgcctc ccatggacaa tgatgattta cgccgaggta agcctacaaa
      661 ccacaaggtg ttcggtgaag gcactgctgt tcttgcgggg gatgcacttc tttcatttgc
      721 atttgagcac attgcagtgt ccacaagcaa gactgtggag tctgataggg ttttgagggt
      781 ggtatctgaa ttgggtagag caataggctc ggaaggggtt gcaggtgggc aggttgctga
      841 tattaccagt caggggaatc cttctgttgg ccttgagact ctggaatgga ttcacattca
      901 caagactgca gtgctcttgg agtgctccgt tgctagtggg gcgatcatcg gtggtgcttc
      961 tgatgatgag attgagagag ttcgaaagta tgcccgttgc gtggggcttc tgtttcaggt
     1021 tgtggatgac attctcgatg tcacgaaatc atcagaagaa ttgggtaaga ctgcagcaaa
     1081 ggatttgctt agtgataagg ccacttatcc caagctcatg ggtttggaga aagcaaagga
     1141 gtttgctgat gaattgttgg gcaaagctaa ggaggagtta tctttcttca acccaactaa
     1201 ggctgcacct ctgttgggtc ttgcagatta cattgcacaa agacagaact gagcttcgag
     1261 accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
     1321 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     1381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1501 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     1561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2281 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2341 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     2401 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     2461 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     2521 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     2581 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     2641 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     2701 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     2761 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     2821 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     2881 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     2941 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     3001 caaaatgttc tt
//
