LOCUS       sequence-348387-        3672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0063
FEATURES             Location/Qualifiers
     source          1..3672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(92..186)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(207..866)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     781..800
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1028..1046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1050..1093)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             2422..2433
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      2741..2798
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2822..2839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2993..3581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3073..3092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
       61 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
      121 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      181 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      241 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      301 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      361 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      421 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      481 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      541 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      601 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
      661 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      721 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      781 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      841 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
      901 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      961 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1021 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     1081 taaggatgat ttctggaatt cggtctcaaa tgtctctgcc tttggcaact ccattgatca
     1141 aagacaatga atctctcatc aagttcttgc gccaacccct ggtgcttcct catgaggttg
     1201 atgacagcac aaaaaggagg gaattgttgg aaagaacaag aaaagaacta gaattaaatg
     1261 cagaaaaacc attggaggcc ttgaagatga tagatataat tcaaagattg ggattatcat
     1321 atcattttga agatgatatt aattcaattc tcacaggatt ttcaaatatt agcagccaaa
     1381 ctcatgaaga tctcctcact gcttcacttt gttttcgatt gcttcgacac aatgggcata
     1441 agatcaatcc tgatatattc caaaaattca tggacaacaa tggaaagttt aaagattcat
     1501 taaaggatga cacattaggc atgttaagct tatatgaagc ttcatatttg ggagccaatg
     1561 gagaagaaat attgatggaa gcccaagaat tcaccaaaac tcacctgaaa aactcattgc
     1621 cagccatggc accatctctt tctaagaagg tttctcaagc tttagagcaa ccaagacata
     1681 gaagaatgtt gaggttagaa gctagaagat ttattgaaga atatggtgct gaaaatgacc
     1741 ataatccaga ccttcttgag cttgcaaaat tggattataa caaagtccaa tctctacacc
     1801 aaatggaatt gtctgagata acaaggtggt ggaaacaatt agggcttgtg gataaactca
     1861 cctttgctcg agatcgaccc cttgaatgct ttctttggac agtgggatta ttaccagagc
     1921 ctaagtattc aggttgcaga attgagcttg caaaaaccat agccattttg cttgtcattg
     1981 atgatatctt tgatactcat ggtaccctag atgagcttct tctattcact aatgccatta
     2041 aaagatggga tcttgaggcc atggaagatt taccagaata tatgagaatt tgttacatgg
     2101 cattgtacaa tactactaat gaaatttgct ataaagttct taaggaaaat ggttggagtg
     2161 ttcttcctta cctaaaggca acgtggattg atatgattga aggattcatg gttgaagcag
     2221 aatggttcaa ttctgattat gtaccaaaca tggaagaata tgtagaaaat ggagttagaa
     2281 cagcaggatc atatatggcc ttagtccatt tgttctttct aatagggcaa ggtgtcactg
     2341 aagataatgt gaaattactg attaaaccct atccaaagct cttttcctcc tcaggaagaa
     2401 tccttcgcct ttgggatgat ttgggaactg caaaggagga acaagaaaga ggagacttgg
     2461 catcaagcat acaattgttt atgagagaga aagagataaa atcagaagaa gaaggaagga
     2521 aaggaatatt ggaaattata gagaatttat ggaaagaatt gaatggagaa ttagtttata
     2581 gagaagaaat gcctcttgca ataatcaaga cagcattcaa catggcaaga gcttcccaag
     2641 ttgtgtatca acatgaagag gacacctatt tttcaagtgt agataattat gtaaaagctt
     2701 tgttttttac accttgtttt taagcttcga gaccctgcag tccggcaaaa aagggcaagg
     2761 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
     2821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
     3001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3661 tatcaaaaag ga
//
