LOCUS       sequence-348389-        3195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0059
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(58..152)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(173..832)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     747..766
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     994..1012
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1016..1059)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2230..2287
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2311..2328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2482..3070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2562..2581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
       61 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      121 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      181 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      241 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      301 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      361 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      421 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      481 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      541 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
      601 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
      661 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      721 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      781 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
      841 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
      901 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
      961 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     1021 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt ctcaaatgac
     1081 caagaccaat tcccctgctc cttctgtcat cacttgcaaa gctgctgtgg tatggaaatc
     1141 aggggagcca ccaaaggtgg aagagataca agttgatcca ccaaaggcct cagaagttag
     1201 gattaagatg ctttgtgcca gtttgtgcca cactgatttc cttgcctgca atggccttcc
     1261 tgttccattg ttccctcgca ttcctggaca cgaaggagtc ggaatgatcg agagcgttgg
     1321 agaaaatgtt acgaacctaa aagaaggaga catagtgatg ccactttact tgggagaatg
     1381 tggggaatgc ttgaattgca aatcaggaag gacaaatttg tgccacaaat atcctttagg
     1441 ttttagtgga ttattgcttg atggaacatc aagaatgtca attggagagc aaaaagtata
     1501 tcaccatttc agctgttcga catggtcaga gtatatagtg attgaagcag cttatgcagt
     1561 gaaggtagat ccaagggttt ctcttccaca tgctagcttt ctttgctgtg gattcaccac
     1621 tggttttggt gctacttgga gagatgtcaa tgttgtcaag ggctctactg tggctgttct
     1681 aggccttggt gctgttggac ttggggctgt gcaaggagct aaatcacaag gagcatcaag
     1741 aattataggt ttggatatca acgacaagaa acgtgaaaag ggagaagcat ttggaatgac
     1801 tgaatttata aatccaaaag gttcaaacaa atcaatttct gaactaataa atgaagcaac
     1861 tggtggatta ggacttgact atgtctacga atgcactgga gttccagctt tactcaatga
     1921 agccattgaa tcctctaaag tgggacttgg aactgcagta ttgattgggg caggacttga
     1981 aacaagtgga gaaatcaaat tcattcctct tctgtgtggt agaactgtta aaggttcaat
     2041 ttatggtgga gtaagaccta aatcagacct ccccacttta attgaaaagt gcataaacaa
     2101 ggaaattcca atggatgaac taatgactca tgaagtttca ttgtctgaga taaacaaagg
     2161 atttgagtac cttaagcatc ctgattgtgt caaagttgtt atcaagttct aagcttcgag
     2221 accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
     2281 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     2341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2461 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     2521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3181 aaattaaaaa tgaag
//
