LOCUS       sequence-348391-       10781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded green fluorescent potassium ion indicator GINKO2
            and red fluorescent pH indicator pHuji fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Wu SY, Wen Y, Serre NBC, Laursen CCH, Dietz AG, Taylor BR, Drobizhev
            M, Molina RS, Aggarwal A, Rancic V, Becker M, Ballanyi K, Podgorski 
            K, Hirase H, Nedergaard M, Fendrych M, Lemieux MJ, Eberl DF, Kay AR,
            Campbell RE, Shen Y
  TITLE     A sensitive and specific genetically-encoded potassium ion biosensor
            for in vivo applications across the tree of life.
  JOURNAL   PLoS Biol. 2022 Sep 6;20(9):e3001772. doi: 
            10.1371/journal.pbio.3001772. eCollection 2022 Sep.
  PUBMED    36067248
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2022-09-6"; volume: "20(9):e3001772. doi"; pages: " 
            10.1371/journal.pbio.3001772. eCollection 2022 Sep"
COMMENT     Sequence Label: pUAST-pHuji-GINKO2
FEATURES             Location/Qualifiers
     source          1..10781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(39..69,70..271)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    333..427
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        446..684
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     631..652
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             704..1411
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1117..1136
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1487..1508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1748..1767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2542..2563
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          2704..2769
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2899..2919
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3191..3325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3228..3247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3282..3301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3352..3370)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3822..3893,4295..4568,4643..5297,5359..5674,5878..6009,
                     6080..6694)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7473..8027,8028..8058)
                     /label=P element 5' end
     rep_origin      complement(8293..8881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8373..8392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9052..9843,9844..9912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9675..9694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9913..10017)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10085..10103
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10141..10163)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10263..10282
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcgagcacc cggaagctca cgatgagaat ggccagacca tgatgaaata acataaggtg
       61 gtcccgtcgg caagagacat ccacttaacg tatgcttgca ataagtgcga gtgaaaggaa
      121 tagtattctg agtgtcgtat tgagtctgag tgagacagcg atatgattgt tgattaaccc
      181 ttagcatgtc cgtggggttt gaattaactc ataatattaa ttagacgaaa ttatttttaa
      241 agttttattt ttaataattt gcgagtacgc aaagcttggc tgcatccaac gcgttgggag
      301 ctctccggat ccaagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact
      361 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
      421 ctccgagcgg agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc
      481 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
      541 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
      601 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
      661 attgaataca agaagagaac tctgaatagg gaattgggaa ttcatggtga gcaagggcga
      721 ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      781 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt
      841 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      901 gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga
      961 ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga
     1021 cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa
     1081 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     1141 gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat
     1201 taagtatagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
     1261 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtggacatca agttggacat
     1321 cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1381 ctccaccggc ggcatggacg agctgtacaa gggcggtaca ggaggtagta ctggctcgag
     1441 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1501 cggcgacgta aacggccaca agttcagcgt gtacggcgag ggtgagggcg atgccaccta
     1561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1621 tctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac acccaggagc gcaccatctt
     1741 cttcgaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1801 ggtgaaccgc atcgagctgg agggcatcga cttcaaggag gacggcaaca ttctggggca
     1861 caagctggag tacaacctgc cggacggcct gttcaacttc gtgaaggacg ccggcgagaa
     1921 gctgtgggat gccgtgacag gccagcacga caaggacgac caggccaaga aggtgtggga
     1981 acacctgcac aagaccggca tccccgacgc cgacaaagtg aacatccagg tcgccgacgg
     2041 caaggccaca gtggcaggcg acggactgag ccaggaagcc aaagaaaaga tcctggtggc
     2101 cgtgggcaac atcagcggaa tcgccagcgt ggacgaccaa gtgaaaaccg ccacccctgc
     2161 caccgccagc cagttctaca cagtgaagtc cggcgacacc ctgagcgcca tcagcaatca
     2221 ggtgtacggc aacgccgacc tgtacaacaa gattttcgag gccaacaagc ccatgctgaa
     2281 gtcccccgac aaaatctacc ccggccaggt gctgagaatc ccctatgctg ctaataacgt
     2341 ctatatcaag gccgacaagg agaagaacgg catcaaggcg aacttcaaga tccgccacaa
     2401 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga
     2461 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccagac tttctaaaga
     2521 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2581 tctcggcatg gacgagctgt acatgtaact cgagggtacc tctagaggat ctttgtgaag
     2641 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     2701 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     2761 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     2821 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     2881 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     2941 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     3001 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     3061 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     3121 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3181 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3301 caatgtatct tatcatgtct ggatccacta gaaggcctaa ttctagtatg tatgtaagtt
     3361 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
     3421 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
     3481 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     3541 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     3601 tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca
     3661 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     3721 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     3781 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     3841 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     3901 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     3961 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa
     4021 tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga
     4081 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa
     4141 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     4201 aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct
     4261 tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag
     4321 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc
     4381 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     4441 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     4501 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     4561 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     4621 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     4681 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     4741 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     4801 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     4861 acggccaggg aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat
     4921 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     4981 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     5041 ctggcattcg cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc
     5101 tccggactgg actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     5161 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     5221 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     5281 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac
     5341 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     5401 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     5461 caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc
     5521 caccaaaaat ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     5581 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     5641 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     5701 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt
     5761 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa
     5821 taaatttcat ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg
     5881 gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg
     5941 aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc
     6001 acgataaatg taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta
     6061 atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg
     6121 aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct
     6181 ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc
     6241 cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac
     6301 gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg
     6361 tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt
     6421 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     6481 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     6541 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     6601 gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct
     6661 ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt
     6721 gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     6781 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     6841 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
     6901 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
     6961 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
     7021 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
     7081 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt
     7141 ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc
     7201 caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt
     7261 tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt
     7321 gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg
     7381 ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat
     7441 gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta
     7501 ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct
     7561 aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa acttactctg
     7621 tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt
     7681 tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg
     7741 cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc
     7801 tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta
     7861 attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca
     7921 cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag
     7981 caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc
     8041 ttatgttatt tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa
     8101 gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca
     8161 gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta
     8221 acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa
     8281 gaagaaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10021 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10081 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10141 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10201 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10261 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcaccg
    10321 aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc
    10381 ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat
    10441 tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat
    10501 gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag
    10561 acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc
    10621 atatcttgtt tttattggca caaatataat tacattagct ttttgagggg gcaataaaca
    10681 gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc
    10741 ggctactcct tgcgtcgggc ccgaagtctt agagccagat a
//
