LOCUS       sequence-348434-       11684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-TbID-tagged mouse Lbr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Cheng LC, Zhang X, Abhinav K, Nguyen JA, Baboo S, Martinez-Bartolome
            S, Branon TC, Ting AY, Loose E, Yates JR 3rd, Gerace L
  TITLE     Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor 
            Revealed by Proximity Labeling and Quantitative Proteomics.
  JOURNAL   J Proteome Res. 2022 Sep 2;21(9):2197-2210. doi: 
            10.1021/acs.jproteome.2c00281. Epub 2022 Aug 16.
  PUBMED    35972904
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jproteome.2c00281"; journalName: "J Proteome Res"; 
            date: "2022-09-2- 2"; volume: "21"; issue: "9"; pages: "2197-2210"
COMMENT     Sequence Label: pLV V5-TbID-LBR
FEATURES             Location/Qualifiers
     source          1..11684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1972..2106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2009..2028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2063..2082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2274..2907
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2954..3079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3576..3809
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3994..4038
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4301..4416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        4494..4705
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4706..5644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5601..5621
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5660..5669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5669..5710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5732..6691
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    8751..9300
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8895..8912)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9122..9141
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9303..9902
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9303..9322)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9799..9819
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9926..10514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9979..9999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10397..10408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10721..11354
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11482..11498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11482..11498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11495..11517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11506..11522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11530..11536,11537..11554,11555..11560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11575..11596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
     1981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2101 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     2161 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     2221 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
     2281 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
     2341 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     2401 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     2461 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     2521 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     2581 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     2641 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     2701 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2761 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2821 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2881 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     2941 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     3001 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     3061 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     3121 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     3181 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     3241 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     3301 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     3361 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     3421 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     3481 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     3541 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     3601 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     3661 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     3721 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     3781 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     3841 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     3901 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     3961 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     4021 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     4081 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     4141 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     4201 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     4261 agatccattc gattagtgaa cggatctcga cggtatcgcc tttaaaagaa aaggggggat
     4321 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4381 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     4441 agatccagtt tatcgatgag gccctttcgt cttcactcga ggtgcccgtc agtgggcaga
     4501 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     4561 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4621 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4681 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     4741 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     4801 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     4861 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     4921 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     4981 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5041 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5101 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5161 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5221 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5281 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5341 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     5401 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     5461 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     5521 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     5581 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     5641 tcaggtgtcg tgaggatccg ccgccatggg taagcctatc cctaaccctc tcctcggtct
     5701 cgattctacg gggggcggag ggggtgggcc catgaaagac aatactgtgc ctctgaagct
     5761 gatcgctctc ctggctaatg gcgagttcca tagtggcgaa cagctgggag aaaccctggg
     5821 catgtccagg gccgctatca acaagcacat tcagactctg cgcgactggg gcgtggacgt
     5881 gttcaccgtg cccggaaagg gctactctct gcccgagcct atcccgctgc tgaacgctaa
     5941 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     6001 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     6061 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     6121 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     6181 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     6241 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     6301 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     6361 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     6421 taacctggac aggaatactc tggccgctac gctgatccga gagctgcggg cagccctgga
     6481 actgttcgag caggaaggcc tggctccata tctgccacgg tgggagaagc tggataactt
     6541 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     6601 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     6661 cggagaaatc agtctgcggt ctgccgaaaa gggcggatca gggtcgggtg gctctggatc
     6721 aggcggatct gggtcgatgc caagtaggaa gtttgttgag ggtgaagtgg tcagaggccg
     6781 atggcctggg agctcccttt attatgaagt agagattctg agccacgaca acaaatccca
     6841 gctctacact gtaaaataca aagatggcac cgaactggaa ctgaaggaga gtgatatcaa
     6901 gcctttaaaa tcctttaagc aaagaaaaag tggctcgatt tccagctctc cctctcgacg
     6961 ccgtggcagc cgcagccgca gccgctcaag atcacgttcc cgatccccgg gcagagcacc
     7021 gaaaggctcc agaagatccg tctctgcttc ccatgagggt gacgtgaagg agaagaagga
     7081 gaaggagatg aggagggaaa ttctgcaagt taagctgact ccgcttgtgt tgaagccgtt
     7141 cggaaacagc gtcagcgtgt acaacgggga gcccgaacac atggagaaga acgctacgcc
     7201 gtataaagac aagcaggaaa gaatcatttt gtcaacagaa gacagatata tagttacaca
     7261 gtacagcctt cgtcccagga gagaagaggt caaagccaaa gaaatagagt ctgaggaaca
     7321 aaaccttgtc accaaaggac ccgcaccttt gggaaccttt caagtgacca ctccacagag
     7381 gaaggacttg gagtttggag gagtacctgg tgcggtcctc atcatgctgg gcctgcccgc
     7441 ctgcgtcctc ttgctgctgc tgcagtgcag gcagaaggac ccggggctgc tccacttccc
     7501 tccacctctg ccggctctcc acgagctgtg ggaacccagg gtgtgcggcg tttacctcct
     7561 ctggtttttc gttcaagctc tcttccacct tctaccagtt gggaaggttg cagaaggaac
     7621 acctcttgtt gatggaagaa gactccagta tagactgaat ggattgtatg ccttcattct
     7681 gacatctgca gccctgggag cagctgtctt ctggggtgtt gagctgtgct acctgtacac
     7741 tcacttcctg cagttggcac tggcagccac cgggttctcc gtgctcctga gtgcttacct
     7801 ctatgttcgc tctctgcgag ccccgaggga ggagctgtcg ccagccagtt ccggaaatgc
     7861 tgtctacgac ttcttcattg gccgagagtt aaacccgcga cttggtgctt tcgatctcaa
     7921 gttcttttgt gagttgcgcc ctggattgat tggatgggtg gttattaact tagtgatgct
     7981 tctgatggaa atgaaaattc aggagcgagc tgctccgtcc ttggcaatga tcttggttaa
     8041 cagtttccag ctcttatatg tggtggacgc cctctggaat gaggaagcac tgctgacctc
     8101 catggacatc atgcacgacg gctttggctt catgctggcc tttggggatt tagtgtgggt
     8161 tccatttacc tacagcctcc aggcctttta cctggtcagc catccccatg acttgtcctg
     8221 gccattggct tctgtcatca ttgctctgaa attgtgtgga tatgtaatct tccgttgtgc
     8281 aaactctcag aaaaatgcat tccggaagaa tcccactgat ccaaagcttg cacatttgaa
     8341 gaccattcat acttccacgg ggaaaagtct gcttgtttcc ggatggtggg gctttgttcg
     8401 ccatcccaat tacttgggtg acctcatcat ggctctggcg tggtccctcc catgtggttt
     8461 caaccacctc ctgccatact tctacattat ctacttcacc gccttgctta tccatcgaga
     8521 ggcccgggat gaacaccagt gcaggaggaa gtacggcctg gcctgggaga agtactgcca
     8581 gcgagtgccc taccgcatat tcccctacat ttactgagtt taaacttcga aacgcgtgaa
     8641 ttcctcgagg gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta
     8701 agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg
     8761 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     8821 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     8881 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8941 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     9001 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     9061 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     9121 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     9181 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     9241 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     9301 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9361 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9421 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9481 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     9541 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9601 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9661 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9721 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9781 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9841 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     9901 gacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     9961 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    10021 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    10081 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    10141 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    10201 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    10261 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    10321 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    10381 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    10441 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    10501 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
    10561 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
    10621 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
    10681 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
    10741 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10801 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    10861 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    10921 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    10981 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    11041 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    11101 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    11161 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11221 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11281 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11341 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    11401 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag
    11461 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    11521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    11581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    11641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcg
//
