LOCUS       sequence-348450-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the reversibly switchable fluorescent protein Padron2 in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Konen T, Stumpf D, Grotjohann T, Jansen I, Bossi M, Weber M, Jensen 
            N, Hell SW, Jakobs S
  TITLE     The Positive Switching Fluorescent Protein Padron2 Enables Live-Cell
            Reversible Saturable Optical Linear Fluorescence Transitions 
            (RESOLFT) Nanoscopy without Sequential Illumination Steps.
  JOURNAL   ACS Nano. 2021 May 21. doi: 10.1021/acsnano.0c08207.
  PUBMED    34019380
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2021 May 21. doi: 10.1021/acsnano.0c08207."
COMMENT     Sequence Label: pBAD-Padron2
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..696
                     /codon_start=1
                     /product="fast-switching photoswitchable fluorescent 
                     protein useful for fluorescence nanoscopy (Tiwari et al., 
                     2015)"
                     /label=Kohinoor
                     /translation="MAVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSVDLKVK
                     EGGPLPFAYDILSMAFCYGNKVFIKYPENIVDYFKQLFPEGYSWERSMIYEDGGICNAT
                     NDITLDGDCFIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSL
                     EGGGHYRCDSKTTYKAKKVVQLPDYHAVDHHIEIKSHDKDYSNVNLHEHAEAHSGLPGQ
                     A"
ORIGIN
        1 atggtgagca agggcgagga gaacaacatg gccgtgatta aaccagacat gaagatcaag
       61 ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga ttgaaggagt tggccttggg
      121 aagcctttcg agggaaaaca gagtgtggac cttaaagtca aagaaggcgg acctctgcct
      181 ttcgcctatg acatcttgtc catggcgttc tgttacggca acaaggtatt catcaaatac
      241 ccagaaaata tagtagacta tttcaagcag ctttttcctg agggctactc ttgggaacga
      301 agcatgattt acgaagacgg gggcatttgt aacgcgacaa acgacataac cctggatggt
      361 gactgtttta tctatgaaat tcgatttgat ggtgtgaact ttcctgccaa tggtccagtt
      421 atgcagaaga ggactgtgaa atgggagcca tccactgaga aattgtatgt gcgtgatgga
      481 gtgctgaagt ctgatggtaa ctacgctctg tcgcttgaag gaggtggcca ttaccgatgt
      541 gactccaaaa ctacttataa agctaagaag gttgtccagt tgccagacta tcacgctgtg
      601 gaccaccaca ttgagattaa aagccacgac aaagattaca gtaatgttaa tctgcatgag
      661 catgccgaag cgcattctgg gctgccgggg caggccatgg acgagctgta caagtaa
//
