LOCUS       sequence-348453-        4979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic 3X LXRE promoter linked to luciferase in the pTAL vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Griffett K, Hayes M, Bedia-Diaz G, Appourchaux K, Sanders R, 
            Boeckman MP, Koelblen T, Zhang J, Schulman IG, Elgendy B, Burris TP
  TITLE     Antihyperlipidemic Activity of Gut-Restricted LXR Inverse Agonists.
  JOURNAL   ACS Chem Biol. 2022 Apr 13. doi: 10.1021/acschembio.2c00057.
  PUBMED    35417135
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2022 Apr 13. doi: 10.1021/acschembio.2c00057."
COMMENT     Sequence Label: LXRE_Luc
FEATURES             Location/Qualifiers
     source          1..4979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(230..1021,1022..1090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     853..872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1091..1195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1222..1677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1309..1328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1519..1540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1808..1856
                     /note="synthetic polyadenylation signal"
     misc_feature    1870..1961
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1910..1929
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2211..2220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2217..3869
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2283..2301)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3910..4031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3921..3940)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3975..3994
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4279..4296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4450..4979,1..59))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4530..4549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
       61 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      241 taatcagtga ggcacctatc tcagcgatct gtctattacg ttcatccata gttgcctgac
      301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1201 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1561 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1621 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
     1681 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1741 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
     1801 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     1861 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     1921 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgatagg taccaggtta
     1981 ctgctggtca ggaggttact gctggtcacc aggttactgc tggtcaagat ctgccgcccc
     2041 gactgcatct gcgtgttcga attcgccaat gacaagacgc tgggcggggt ttgtgtcatc
     2101 atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa acgcgagcaa
     2161 cgggccacgg ggatgaagca gaagcttggc attccggtac tgttggtaaa gccaccatgg
     2221 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
     2281 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2341 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
     2401 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     2461 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     2521 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
     2581 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     2641 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
     2701 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     2761 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
     2821 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
     2881 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     2941 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3001 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     3061 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     3121 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     3181 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     3241 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3301 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3361 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     3421 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     3481 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     3541 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     3601 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     3661 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     3721 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     3781 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     3841 ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag
     3901 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3961 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4021 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     4081 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     4141 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     4201 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     4261 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctg
//
