LOCUS       sequence-348455-        5770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloned human Soat2 promoter containing exon1 and 2 coupled to 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Griffett K, Hayes M, Bedia-Diaz G, Appourchaux K, Sanders R, 
            Boeckman MP, Koelblen T, Zhang J, Schulman IG, Elgendy B, Burris TP
  TITLE     Antihyperlipidemic Activity of Gut-Restricted LXR Inverse Agonists.
  JOURNAL   ACS Chem Biol. 2022 Apr 13. doi: 10.1021/acschembio.2c00057.
  PUBMED    35417135
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2022 Apr 13. doi: 10.1021/acschembio.2c00057."
COMMENT     Sequence Label: Soat2_Luc
FEATURES             Location/Qualifiers
     source          1..5770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      34..489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(121..140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     331..352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    620..668
                     /note="synthetic polyadenylation signal"
     misc_feature    682..773
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     722..741
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             1820..3472
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1886..1904)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3513..3634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3524..3543)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3578..3597
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3882..3899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4053..4641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4133..4152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4812..5603,5604..5672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5435..5454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5673..5770,1..7))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
       61 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      121 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      181 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      241 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      301 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      361 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      421 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      481 ttacaatttg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      541 cctcttcgct attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg
      601 aggtacttgg agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
      661 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
      721 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
      781 ggtaccgagc tcttacgcgt gctagcccgg gctcgagccc aagatgtacc cagccactat
      841 tcctgtgtgt gtgtggcggg gtggatagca cccttggagc tgggcatctg gatggtggtt
      901 ggggtgttag ggggatgtgg cgatccctgc gacagacagc agagtgaggt ctgaatctga
      961 gaagctctgc ctccagatca gataacctat cgcactccca gaggccccct cctcctgaaa
     1021 ggactttagt ctttggagct gtcacctgag ctgagtggga caagagctct acagggcagg
     1081 ccacactgcg aaggaaggag gcaacacggg caagggctgc ctgctgcccg ctggagaccg
     1141 caccatggag ccaggcgggg cccgtctgcg tctgcagagg acagaagggc tgggagggga
     1201 gcgggagcgc caaccctgtg gagatggtga gccgccctcg ggggtgcaga aggcacaggc
     1261 aagtgggggg gaggcgggga gagatgcgct atggagagaa ggctccaact gcctgatgcc
     1321 aatccttcct gctgtccaga gaggccaagg aaggtagacc ctggcctcag cccagagcca
     1381 cccagctgga agatggaaca gaaaagagct atgtcagagc tgtggcctgg ccttatcctt
     1441 ctgactgttt agccccagat agtgaatgga gtagcttccc attccctgag tgccctgttc
     1501 ccagtaatgt caccaggccc cctgcttgcc tatggcctcc tcaccactgg caacagcaac
     1561 ttccccttct agtagcccca accatgatac tagatattgg ctggttgggg tgagggcagc
     1621 tgctgagcac acaaagcatt tcctgaccac aggatgcctc tgggtctgca gaaccccaat
     1681 tcctcctgtt gactgtgcct ttgatccctc ctcacaggaa acactgagac gcacagagcc
     1741 ccggacttgg tacaatggac ccgacacatg gaggtgaggg atgtcagagg tcagaggtca
     1801 agtggacaga tccttggcca tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     1861 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     1921 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
     1981 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     2041 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     2101 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     2161 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
     2221 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
     2281 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     2341 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
     2401 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
     2461 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     2521 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     2581 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
     2641 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
     2701 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     2761 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     2821 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     2881 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     2941 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     3001 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     3061 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     3121 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     3181 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     3241 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     3301 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     3361 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     3421 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagag
     3481 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     3541 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3601 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3661 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     3721 tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     3781 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     3841 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     3901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4021 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4561 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4621 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4681 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4741 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4801 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4861 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4921 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4981 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5041 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5101 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5161 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5221 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5281 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5341 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5401 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5461 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5521 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5581 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5641 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5761 caaatagggg
//
