LOCUS       sequence-348466-       10965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express mCherry fused to PTDSS1, a protein that localized to 
            mitochondria associated ER membranes (MAMs). Lentiviral vector used 
            to make cell lines expressing this MAM landmark..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10965)
  TITLE     David Andrews Lab plasmids
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
FEATURES             Location/Qualifiers
     source          1..10965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3553..4260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3698..3716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3966..3985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(5715..6289,6290..6292,6293..6301)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5882..5899)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6109..6128
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6321..6920
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6321..6340)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6817..6837
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6934..7522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6987..7007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7405..7416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7729..8362
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8490..8506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8490..8506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8503..8525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8514..8530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8538..8544,8545..8562,8563..8568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8583..8604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8721..8738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8892..9480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8972..8991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9651..10442,10443..10511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10274..10293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10512..10616)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10664..10798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10701..10720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10755..10774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gtatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     4021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     4081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4261 tccggactca gatctggtac tagtatggcg tcctgcgtgg ggagccggac cctaagcaag
     4321 gatgatgtga actacaaaat gcatttccgg atgatcaacg agcagcaagt ggaggacatc
     4381 accattgact tcttctaccg gccgcatacc atcaccctgc tcagcttcac catcgtcagc
     4441 ctcatgtact tcgcctttac cagggatgac tctgttccag aagacaacat ctggagaggc
     4501 atcctctctg ttattttctt ctttcttatc atcagtgtgt tagctttccc caatggtccg
     4561 ttcactcgac ctcatccagc cttatggcga atggtttttg gactcagtgt gctctacttc
     4621 ctgttcctgg tattcctact cttcctgaat ttcgagcagg ttaaatctct aatgtattgg
     4681 ctagatccaa atcttcgata cgccacaagg gaagcagatg tcatggagta tgctgtgaac
     4741 tgccatgtga tcacctggga gaggattatc agccactttg atatttttgc atttggacat
     4801 ttctggggct gggccatgaa ggccttgctg atccgtagtt acggtctctg ctggacaatc
     4861 agtattacct gggagctgac tgagctcttc ttcatgcatc tcctccccaa ttttgccgag
     4921 tgctggtggg atcaagtcat tctggacatc ctgttgtgca atggcggtgg catttggctg
     4981 ggcatggtcg tttgccggtt tttagagatg aggacttacc actgggcaag cttcaaggac
     5041 attcatacca ccaccgggaa gatcaagaga gctgttctgc agttcactcc tgctagctgg
     5101 acctatgttc gatggtttga ccccaaatct tcttttcaga gagtagctgg agtgtacctt
     5161 ttcatgatca tctggcagct gactgagttg aataccttct tcttgaagca tatctttgtg
     5221 ttccaagcca gtcatccatt aagttggggt agaattctct ttattggtgg catcacagct
     5281 cccacagtga gacagtacta cgcttacctc accgacacac agtgcaagcg cgtaggaaca
     5341 caatgctggg tgtttggggt cattggtttc ctggaggcca ttgtttgcat aaaatttgga
     5401 caagatctct tctctaagac ccaaatactc tatgttgtgc tttggcttct ttgcgtggct
     5461 ttcaccactt tcctctgtct gtacggcatg atttggtatg cagaacacta tggtcaccga
     5521 gaaaagacct actcggagtg tgaagatggc acctacagtc cagagatctc ctggcatcac
     5581 aggaaaggga caaaaggttc tgaagacagc ccacccaagc atgcaggcaa caacgaaagc
     5641 cattcttcca ggagaaggaa tcggcattcc aagtcaaaag tcaccaatgg cgttggaaag
     5701 aaatgaggat cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     5761 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5821 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5881 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5941 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     6001 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     6061 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     6121 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     6181 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6241 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     6301 ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6361 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6421 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6481 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6541 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6601 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6661 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6721 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6781 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6841 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6901 cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt
     6961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7321 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7501 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     7561 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     7621 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     7681 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     7741 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     7801 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     7861 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     7921 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     7981 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     8041 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     8101 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     8161 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8341 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8401 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     8461 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10621 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    10681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10801 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10861 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10921 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
