LOCUS       sequence-348479-       11743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3163
FEATURES             Location/Qualifiers
     source          1..11743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4497..4521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4529..4570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4681..5269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4734..4754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5152..5163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5341..5574
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5652..5773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5689..5708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5743..5762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5813..5948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5968..5987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5969..5987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5997..6014)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5997..6013)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6006..6028)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6155..6610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6242..6261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6452..6473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6636..6740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6741..6809,6810..7601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6959..6978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7772..8360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8261..8280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8514..8531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8648..8669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8684..8689,8690..8707,8708..8714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8722..8738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8727..8749
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8746..8762
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8746..8762
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8781..8801
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8783..8801
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8829..9055
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9056..9236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9283..9408
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9901..10134
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10319..10363
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10594..10804
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10616..10812
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10663..10798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10844..11446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10844..10863)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11340..11360
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11489..11606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgat gccgatgata ttaactgttt tcttgagcaa
     1261 caatgaacag attttaacag aagttcctat aacaccggaa acaacctgtc gagatgttgt
     1321 agaattttgc aaggaacctg gagaaggcag ctgccattta gctgaagtgt ggaggggaaa
     1381 tgaacgtccc ataccctttg atcatatgat gtacgaacat cttcagaaat ggggtccacg
     1441 gagggaagaa gtgaaatttt tccttcgaca cgaggactcc ccaactgaga acagtgaaca
     1501 aggtggccgt cagacccaag agcaacgaac tcagagaaat gtaataaatg tacctggaga
     1561 aaaacgtact gaaaatgggg ttgggaatcc acgtgttgaa cttaccctct cagagctcca
     1621 agatatggca gctaggcaac agcagcagat tgaaaatcag cagcagatgt tggttgccaa
     1681 ggaacagcgt ttacattttc taaagcaaca ggagcgccgt cagcagcagt ctatttctga
     1741 aaatgaaaag cttcagaaat tgaaagaacg agttgaagcc caggagaaca agctgaagaa
     1801 aattcgtgca atgagaggac aagtcgacta cagcaaaatc atgaacggca atctgtctgc
     1861 tgaaatagaa aggttcagtg ccatgttcca ggaaaagaag caggaagtac agactgcaat
     1921 tttaagggtt gatcagctta gtcagcaatt ggaagattta aagaaaggaa aactgaatgg
     1981 gttccagtct tacaatggca aattgacggg accagcggcg gtggagttaa aaagactgta
     2041 ccaagaacta cagattcgta accaacttaa ccaggaacaa aattcaaaac ttcagcagca
     2101 gaaggaactc ttaaataagc gcaacatgga ggtggccatg atggacaagc gaatcagtga
     2161 actgcgtgaa cgtctctatg ggaaaaaaat tcagctgaac cgtgtgaatg gcacgtcatc
     2221 accacagtcc cctctgagca catcgggcag ggtcgctgct gtggggcctt atatccaggt
     2281 tcccagtgcc ggaagctttc ctgtgctggg ggaccctata aagccccagt ctctcagtat
     2341 tgcctcaaat gctgctcatg gaagatccaa atccgctaat gatggaaact ggccaacatt
     2401 aaaacagaat tctagctctt ccgtgaaacc agtgcaggtg gccggtgcag actggaagga
     2461 tccgagcgtg gaggggtctg tcaagcaggg cactgtctcc agccagcctg tgcccttctc
     2521 agcactggga cccacggaga agccgggcat cgagattggt aaagtgccac ctcccatccc
     2581 gggtgtaggc aagcagctgc ctccaagcta tgggacatac ccaagtccta cacctctggg
     2641 tcctgggtcg acaagctccc tggaaaggag gaaggaaggc agcttgccca ggcccagtgc
     2701 aggcctgcca agtcgacaga ggcccaccct gctgcccgcc acaggcagca ccccccagcc
     2761 aggctcctca caacagattc agcagaggat ttccgtaccg ccaagtccca cgtacccgcc
     2821 agcgggacca cctgcatttc cagctgggga cagcaagcct gaactcccac tgacagtggc
     2881 cattaggcct ttcctggctg ataaagggtc aaggccacag tctcccagga aaggacccca
     2941 gacagtgaat tcaagttcca tatactccat gtacctccag caagccacac cacctaagaa
     3001 ttaccagccg gcagcacaca gcgccttaaa taagtcagtt aaagcagtgt atggtaagcc
     3061 cgttttacct tcgggttcaa cctctccatc gccgctgccg tttcttcacg ggtcactgtc
     3121 cacgggcaca ccacagcctc agccaccttc agaaagtact gagaaagagc ctgagcagga
     3181 tggccccgcc gcccccgcag atggcagcac cgtggagagc ctgccacggc cactcagccc
     3241 caccaagctc acgcccatcg tgcattcgcc actgcgctac cagagtgatg cagacctgga
     3301 ggccctccgc aggaagctgg ccaacgcgcc ccggcccctg aaaaagcgca gctccatcac
     3361 agagcccgag ggccccggcg ggcccaacat ccagaagctg ctgtaccagc gcttcaacac
     3421 cctggccggt ggcatggagg gcaccccttt ctaccagccc agcccctccc aggacttcat
     3481 gggcaccttg gccgatgtgg acaatggaaa caccaatgcc aatggaaacc tggaagagct
     3541 cccccctgcc cagcccacag ccccactccc cgctgagcct gccccgtcat cagatgccaa
     3601 tgataatgag ttaccttccc ccgaaccaga ggagctcatc tgtccccaaa ccacccacca
     3661 aactgccgag ccggcagagg acaataacaa caacgtggcc acggtcccca ccacggagca
     3721 gatcccgagt cctgtggctg aggccccatc tccaggggaa gagcaggtcc ctccagcacc
     3781 tcttccccct gccagccacc ctcctgccac ctccacgaac aagcggacca acttgaagaa
     3841 gcccaactcg gagcggacgg ggcacgggct gagagtccgg tttaaccccc tggcactgct
     3901 cctagacgcg tctctggaag gagagttcga tctggtgcag aggatcatct atgaggtgga
     3961 agatcccagc aagcccaacg acgaagggat caccccactg cacaacgccg tctgcgccgg
     4021 ccaccatcac atcgtgaagt tcctgctgga ttttggtgtc aacgtgaatg ctgctgatag
     4081 tgatggatgg acgccgctgc actgcgctgc ctcttgtaac agcgttcacc tctgcaaaca
     4141 gctggtggag agtggtgccg ccatttttgc ctcaaccata agcgacattg aaactgctgc
     4201 agacaagtgt gaggagatgg aggaaggcta catccagtgc tcccagtttc tatatggggt
     4261 gcaggaaaag ctgggtgtga tgaacaaagg tgtggcgtat gctctgtggg actacgaggc
     4321 ccagaacagt gacgagctgt ccttccacga aggggacgcc ctcaccatcc tgaggcgcaa
     4381 ggacgaaagc gagactgagt ggtggtgggc tcgccttgga gaccgggagg gctatgtgcc
     4441 caaaaacctg ctggggctgt atccacggat caaaccccga cagcgaacac tcgccttgcc
     4501 aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     4561 cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     4621 tatatcttgt ggaaaggacg aactgccact gataaactac cgcgtacgcg ttaagtcgac
     4681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5041 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     5281 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5341 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     5581 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5641 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5761 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5821 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5881 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5941 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6001 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6061 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6121 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6181 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6241 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6301 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6361 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6421 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6481 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6541 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6601 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6781 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6841 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6901 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6961 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7021 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7081 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7141 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7201 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7261 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7321 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7381 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7441 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7501 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7561 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7621 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7681 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7741 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7801 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7861 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7921 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7981 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8041 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8101 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8161 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8221 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8281 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8341 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8401 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8461 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8521 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8581 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8641 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8701 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8761 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     8821 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     8881 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     8941 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     9001 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     9061 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9121 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9181 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     9241 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     9301 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     9361 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     9421 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9481 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9541 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     9601 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     9661 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9721 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     9781 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9841 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9901 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9961 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10021 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    10081 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10141 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10201 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10261 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10321 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10381 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10441 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10501 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
    10561 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
    10621 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    10681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10741 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10801 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
    10861 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    10921 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    10981 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    11041 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    11101 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    11161 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    11221 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    11281 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    11341 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    11401 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    11461 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    11521 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    11581 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    11641 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    11701 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
